##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921179_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3481143
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.950077603821505	31.0	30.0	33.0	26.0	34.0
2	30.193469501252892	31.0	30.0	34.0	26.0	34.0
3	30.31623664985897	31.0	30.0	34.0	26.0	34.0
4	34.0257062120114	35.0	35.0	37.0	30.0	37.0
5	33.62946537961813	35.0	35.0	37.0	28.0	37.0
6	33.61254593677996	37.0	35.0	37.0	28.0	37.0
7	33.48174579441293	37.0	35.0	37.0	28.0	37.0
8	33.433830497626786	37.0	35.0	37.0	28.0	37.0
9	34.8660273364237	39.0	35.0	39.0	28.0	39.0
10	34.73894235312942	38.0	35.0	39.0	27.0	39.0
11	34.77695084631686	39.0	35.0	39.0	27.0	39.0
12	34.64927927407751	38.0	35.0	39.0	27.0	39.0
13	34.633951549821425	38.0	35.0	39.0	27.0	39.0
14	35.602267703452576	39.0	36.0	41.0	27.0	41.0
15	35.608907763915475	39.0	36.0	41.0	27.0	41.0
16	35.59477217683962	39.0	36.0	41.0	27.0	41.0
17	35.57676659648857	39.0	36.0	41.0	27.0	41.0
18	35.53041888827894	39.0	36.0	41.0	27.0	41.0
19	35.5849820016012	39.0	36.0	41.0	27.0	41.0
20	35.52259128682734	39.0	36.0	41.0	27.0	41.0
21	35.4570243164386	39.0	36.0	41.0	27.0	41.0
22	35.37523853515928	39.0	35.0	41.0	27.0	41.0
23	35.2567027553881	39.0	35.0	41.0	26.0	41.0
24	35.18757201298539	39.0	35.0	41.0	26.0	41.0
25	35.05609278331858	39.0	35.0	41.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	86222.0
3	9.0
4	1334.0
5	11896.0
6	13553.0
7	10409.0
8	8105.0
9	7089.0
10	6491.0
11	5948.0
12	5647.0
13	5342.0
14	5246.0
15	5272.0
16	5379.0
17	5689.0
18	6422.0
19	7524.0
20	9498.0
21	12246.0
22	15718.0
23	19561.0
24	23217.0
25	27773.0
26	31747.0
27	35838.0
28	42306.0
29	51832.0
30	65902.0
31	85638.0
32	110029.0
33	143941.0
34	191503.0
35	254176.0
36	348317.0
37	526029.0
38	1288295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.080091691098076	19.131602949481334	9.519104804121268	31.269200555299324
2	14.47185593927052	21.164485342888817	36.291413481146854	28.07224523669381
3	17.82128366902493	24.124158471952324	28.279566925943232	29.774990933079515
4	13.103836790535784	16.768260610885804	34.257575714027034	35.87032688455138
5	14.203955530464437	37.82937362527437	33.36511562930096	14.601555214960236
6	33.601588502660434	35.75912076193891	16.67433684552834	13.96495388987232
7	29.431045086705865	32.1115198856006	19.99668499113939	18.46075003655415
8	26.838615082142027	35.879466045243895	18.876693313844928	18.40522555876915
9	26.928885139162627	15.968174820741348	19.073591633552542	38.029348406543484
10	15.967255582433701	29.257488129617197	31.983518057143872	22.79173823080523
11	35.526356233957806	22.8686418341566	22.100886081949294	19.504115849936298
12	23.277498224002862	25.072081210108287	29.43593526608933	22.21448529979952
13	28.507225115128143	21.469836871513515	25.3392800767339	24.683657936624435
14	22.327958169838457	21.032607383510978	26.28874009402129	30.350694352629276
15	24.33901883444354	28.57860748320898	22.85635806129814	24.226015621049342
16	24.224883751593953	27.73935307657902	24.64971792148683	23.386045250340192
17	23.00646655423233	27.70661819982689	26.142419314575697	23.14449593136507
18	22.90382685144701	27.569365883814985	26.733436099733588	22.793371165004423
19	24.133956788114332	26.852431447791503	26.576249061700935	22.437362702393226
20	24.307131307159747	26.487248584732082	26.358785031238302	22.84683507686987
21	24.49626001647914	25.992741823346826	26.33286830132695	23.17812985884708
22	24.39520582751125	25.882935576044996	26.082266657818998	23.639591938624758
23	24.00768258712213	25.954815103993862	26.21099709604871	23.826505212835297
24	23.59804653835105	26.2626544096524	26.47644355070382	23.662855501292732
25	23.73626746763154	26.006620736649033	26.394806312950664	23.86230548276876
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	616.0
1	616.0
2	687.5
3	759.0
4	759.0
5	759.0
6	1183.5
7	1608.0
8	1608.0
9	1608.0
10	2217.0
11	2826.0
12	2826.0
13	2826.0
14	4937.5
15	7049.0
16	7049.0
17	7049.0
18	14585.5
19	22122.0
20	22122.0
21	22122.0
22	38740.0
23	55358.0
24	55358.0
25	55358.0
26	84507.5
27	113657.0
28	113657.0
29	113657.0
30	147889.0
31	182121.0
32	182121.0
33	182121.0
34	220365.0
35	258609.0
36	258609.0
37	258609.0
38	300930.5
39	343252.0
40	343252.0
41	343252.0
42	380107.0
43	416962.0
44	416962.0
45	416962.0
46	445940.5
47	474919.0
48	474919.0
49	474919.0
50	481185.0
51	487451.0
52	487451.0
53	487451.0
54	450005.5
55	412560.0
56	412560.0
57	412560.0
58	361104.0
59	309648.0
60	309648.0
61	309648.0
62	256168.5
63	202689.0
64	202689.0
65	202689.0
66	155778.0
67	108867.0
68	108867.0
69	108867.0
70	79329.5
71	49792.0
72	49792.0
73	49792.0
74	34444.0
75	19096.0
76	19096.0
77	19096.0
78	13395.0
79	7694.0
80	7694.0
81	7694.0
82	5054.5
83	2415.0
84	2415.0
85	2415.0
86	1575.0
87	735.0
88	735.0
89	735.0
90	499.5
91	264.0
92	264.0
93	264.0
94	162.5
95	61.0
96	61.0
97	61.0
98	37.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09706582004818533
2	0.0
3	0.04196897398354506
4	0.055527739021350164
5	0.06512228885742412
6	0.0037056794277052106
7	1.7235718268396327E-4
8	0.0028438935142853943
9	0.0
10	0.0
11	0.0025279053460314617
12	0.0
13	6.319763365078654E-4
14	0.05547028662712219
15	0.02042432614804965
16	5.745239422798777E-4
17	0.0
18	0.003131155485425333
19	0.003159881682539327
20	0.0
21	0.010887228706203681
22	0.0
23	6.894287307358531E-4
24	0.004107846187301124
25	0.1723859088810773
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3481143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.782269747572705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.79013227832096	21.624345039566826
2	15.705153100820915	11.553423555626456
3	8.019682281532377	8.849463509074596
4	4.774650140855977	7.024898777250023
5	3.1295229802206177	5.755547921985119
6	2.159982870856418	4.766944356358636
7	1.526629305651388	3.9307023647513284
8	1.104072999669972	3.248824871589813
9	0.8228739753127495	2.724045527538993
>10	3.7946891498250643	22.893837005129317
>50	0.12048103335500948	3.0247244192678653
>100	0.048437437224183026	3.0585749343857285
>500	0.0024354433437062143	0.6050194197923576
>1k	0.0011784403226589705	0.7848463193120686
>5k	7.85626881772647E-5	0.15480197837094078
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5357	0.15388623793966522	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.8726197113993882E-5	0.0
3	0.0	0.0	0.0	2.8726197113993882E-5	0.0
4	0.0	0.0	0.0	2.8726197113993882E-5	0.0
5	0.0	0.0	0.0	2.8726197113993882E-5	0.0
6	0.0	0.0	0.0	2.8726197113993882E-5	0.0
7	0.0	0.0	0.0	5.7452394227987763E-5	0.0
8	0.0	0.0	0.0	1.1490478845597553E-4	0.0
9	0.0	0.0	0.0	1.436309855699694E-4	0.0
10	0.0	0.0	0.0	1.723571826839633E-4	0.0
11	0.0	0.0	0.0	1.723571826839633E-4	0.0
12	0.0	0.0	0.0	1.723571826839633E-4	0.0
13	0.0	0.0	0.0	1.723571826839633E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2015	0.0	12.652751	1
TCGCGCA	80	2.8876639E-5	11.867378	18
ACCGCTC	120	1.2796954E-7	11.081631	8
CGACAGT	95	1.3718036E-5	10.992467	6
GTTCGCG	80	3.795658E-4	10.68064	16
CTATTAG	265	0.0	10.410638	1
CCGTCCA	235	0.0	10.106574	9
GTATCAA	4305	0.0	10.076668	1
CTACGGG	95	1.6372198E-4	10.004791	3
TAGGACA	460	0.0	9.917793	4
CGTGCGC	260	0.0	9.865572	10
TACGGGT	120	1.692141E-5	9.504551	4
TAGGACT	430	0.0	9.504551	4
CCTATAC	290	0.0	9.504551	3
CTAGACA	415	0.0	9.3900385	4
ATAATAC	315	0.0	9.353685	3
GTATAGG	245	1.8189894E-12	9.319024	1
GTCCTAG	290	0.0	9.185129	1
TACACCG	260	0.0	9.133476	5
GTATAGA	345	0.0	9.099554	1
>>END_MODULE