FastQCFastQC Report
Mon 15 Aug 2016
SRR2921178_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921178_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2073858
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35610.17170895982270726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCT551.9659547E-413.813789
GCGACGA604.099059E-412.66446413
CCCTATA701.09030865E-412.2154032
GGTATCA16050.011.959211
GTTCTAG1803.6379788E-1211.0859511
TCGCGCA1158.8480374E-710.736485518
GACCGTC1605.475158E-1010.683587
GTGCTAT1355.66979E-810.5580491
GTATCAA32300.010.1788751
CCGTCTA750.002655217410.1301059
TAGAACC1606.211849E-910.0900494
TAGCCTA951.6505225E-49.9963325
ACGCATA1151.0311542E-59.9094097
TCGTTCG1252.5762656E-69.87828314
CGTTCGC1252.5762656E-69.87828315
TGGACCG1252.5826248E-69.8763775
AACGCAT1252.5826248E-69.8763776
CTAAGCT1554.087451E-89.8028554
GTCTATA900.00111144069.5022431
CTAGATC1201.7081069E-59.4965164
Produced by FastQC (version 0.11.5)