##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921178_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2073858
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.821841225387658	31.0	27.0	31.0	10.0	34.0
2	26.56543167372115	31.0	26.0	31.0	10.0	34.0
3	26.321461739424784	31.0	26.0	31.0	10.0	34.0
4	30.143194953559984	35.0	30.0	37.0	10.0	37.0
5	29.916629296702087	35.0	30.0	37.0	10.0	37.0
6	30.48120893523086	35.0	31.0	37.0	10.0	37.0
7	30.47619171611557	35.0	31.0	37.0	8.0	37.0
8	30.108635692511253	35.0	30.0	37.0	8.0	37.0
9	31.73023659286219	37.0	31.0	39.0	2.0	39.0
10	31.07974557563729	37.0	30.0	39.0	2.0	39.0
11	31.53758164734519	37.0	31.0	39.0	2.0	39.0
12	31.22510943372208	37.0	30.0	39.0	2.0	39.0
13	31.22922736272204	37.0	30.0	39.0	2.0	39.0
14	32.31196398210485	38.0	31.0	40.0	2.0	41.0
15	31.95837998551492	38.0	31.0	40.0	2.0	41.0
16	31.8841198384846	38.0	31.0	40.0	2.0	41.0
17	32.213631309376055	38.0	31.0	40.0	2.0	41.0
18	32.264506538056125	38.0	32.0	40.0	2.0	41.0
19	31.959579199733056	38.0	31.0	40.0	2.0	41.0
20	31.831054970976798	38.0	31.0	40.0	2.0	41.0
21	31.77320867677536	38.0	31.0	40.0	2.0	41.0
22	31.004355650193986	38.0	29.0	40.0	2.0	41.0
23	31.102591402111425	38.0	30.0	40.0	2.0	41.0
24	30.72162703521649	38.0	30.0	40.0	2.0	41.0
25	30.224263184846794	37.0	28.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	175340.0
3	7.0
4	1198.0
5	6974.0
6	8888.0
7	8054.0
8	6965.0
9	6441.0
10	6218.0
11	5893.0
12	5761.0
13	5672.0
14	5639.0
15	5873.0
16	6148.0
17	6872.0
18	7808.0
19	9700.0
20	12364.0
21	15220.0
22	18680.0
23	21727.0
24	24956.0
25	28031.0
26	31795.0
27	35342.0
28	39544.0
29	45999.0
30	54246.0
31	65359.0
32	81718.0
33	103238.0
34	132302.0
35	171047.0
36	225409.0
37	300104.0
38	387326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.49653354762809	18.068578501290546	12.358928918552719	25.07595903252865
2	16.482248316594564	20.014589433371302	38.31781517445807	25.185347075576065
3	19.221422574125256	23.74622085494883	29.690227054941015	27.342129515984897
4	13.827339579703429	16.42109902870858	34.324863006002516	35.426698385585475
5	14.296943568376685	36.81567855868368	33.747624972271254	15.139752900668382
6	33.834930529905364	34.006013958633304	17.14600244377344	15.013053067687895
7	29.312188039323377	31.823207776720665	20.319908727014788	18.544695456941167
8	26.563565365909643	33.71404819516467	20.40039193410415	19.32199450482154
9	26.98050800420307	15.866728584290188	19.210569589091698	37.94219382241505
10	16.29513863103958	28.142308385950663	32.408242348023755	23.154310634986
11	35.10194121851078	22.860411082701678	21.914733758799716	20.12291393998783
12	23.322113344392577	25.321457505497474	28.7929188688708	22.56351028123915
13	28.77437716791089	21.64600405166964	24.928329040711034	24.65128973970844
14	22.800531459560606	21.43744860829453	25.67282442784906	30.0891955042958
15	24.819490798406918	28.231383549442864	22.968225654706018	23.9808999974442
16	24.038270459241655	27.292108405957038	24.845748980824254	23.823872153977053
17	23.088937930369138	27.713276171870287	25.539789925593624	23.65799597216695
18	23.288545319589062	26.95518513820556	26.613620064078418	23.14264947812696
19	24.37052844793287	26.46507758527146	26.665846806766258	22.49854716002942
20	24.924247429621126	25.81866549121376	26.1714942703905	23.085592808774614
21	25.36855102030973	25.495975190388688	25.781883774880747	23.353590014420828
22	24.410567802123033	24.921096399584712	26.907176574804954	23.761159223487304
23	24.746349937256635	25.38164459958544	25.943441268640953	23.928564194516973
24	24.205030666444188	25.806061143845348	26.13404115158068	23.854867038129786
25	23.883127635012684	25.477881031977905	26.834002738984115	23.804988594025296
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1630.0
1	1630.0
2	1661.5
3	1693.0
4	1693.0
5	1693.0
6	2175.5
7	2658.0
8	2658.0
9	2658.0
10	3318.0
11	3978.0
12	3978.0
13	3978.0
14	5463.0
15	6948.0
16	6948.0
17	6948.0
18	12083.5
19	17219.0
20	17219.0
21	17219.0
22	26025.0
23	34831.0
24	34831.0
25	34831.0
26	50639.0
27	66447.0
28	66447.0
29	66447.0
30	85599.0
31	104751.0
32	104751.0
33	104751.0
34	125461.0
35	146171.0
36	146171.0
37	146171.0
38	169614.5
39	193058.0
40	193058.0
41	193058.0
42	214905.5
43	236753.0
44	236753.0
45	236753.0
46	255727.5
47	274702.0
48	274702.0
49	274702.0
50	278264.5
51	281827.0
52	281827.0
53	281827.0
54	264345.5
55	246864.0
56	246864.0
57	246864.0
58	217973.5
59	189083.0
60	189083.0
61	189083.0
62	158717.5
63	128352.0
64	128352.0
65	128352.0
66	101814.0
67	75276.0
68	75276.0
69	75276.0
70	55967.5
71	36659.0
72	36659.0
73	36659.0
74	26203.5
75	15748.0
76	15748.0
77	15748.0
78	10885.0
79	6022.0
80	6022.0
81	6022.0
82	4115.5
83	2209.0
84	2209.0
85	2209.0
86	1490.0
87	771.0
88	771.0
89	771.0
90	477.5
91	184.0
92	184.0
93	184.0
94	101.5
95	19.0
96	19.0
97	19.0
98	12.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033801735702251555
2	0.05424672277465478
3	0.014803327903839123
4	0.010945783173197008
5	0.009547423208339241
6	7.715089461284234E-4
7	0.0038093254215090908
8	0.0019287723653210586
9	0.0065096067329585726
10	0.003857544730642117
11	0.01022249353620161
12	0.00858303702567871
13	0.006991799824288838
14	0.015574836849967548
15	0.006798922587756732
16	0.029606655807678246
17	0.03775571905115972
18	0.0222291015103252
19	0.01605702994129781
20	0.01596059132303176
21	0.022566636674256384
22	0.006027413641628308
23	0.016008810632164788
24	0.012344143138054774
25	0.4623749552765908
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2073858.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.660296043354215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.6572785270014	32.9688953368762
2	12.011766059366977	11.209451206671549
3	5.7257877509326	8.015008546197793
4	3.2740738363808792	6.1107701789332936
5	2.068342349856603	4.825473318165802
6	1.4416995737976699	4.036207735138612
7	0.989923381924067	3.233308264257076
8	0.73958340228106	2.760734439934832
9	0.5403579246340645	2.269193465951814
>10	2.4377813228702436	18.88619154802258
>50	0.08709819188992095	2.7130845348923938
>100	0.02405376390718068	1.7997554614863225
>500	0.0013952808117359832	0.46995227730520794
>1k	8.586343456836805E-4	0.70197368616657
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3561	0.17170895982270726	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCGCT	55	1.9659547E-4	13.81378	9
GCGACGA	60	4.099059E-4	12.664464	13
CCCTATA	70	1.09030865E-4	12.215403	2
GGTATCA	1605	0.0	11.95921	1
GTTCTAG	180	3.6379788E-12	11.085951	1
TCGCGCA	115	8.8480374E-7	10.7364855	18
GACCGTC	160	5.475158E-10	10.68358	7
GTGCTAT	135	5.66979E-8	10.558049	1
GTATCAA	3230	0.0	10.178875	1
CCGTCTA	75	0.0026552174	10.130105	9
TAGAACC	160	6.211849E-9	10.090049	4
TAGCCTA	95	1.6505225E-4	9.996332	5
ACGCATA	115	1.0311542E-5	9.909409	7
TCGTTCG	125	2.5762656E-6	9.878283	14
CGTTCGC	125	2.5762656E-6	9.878283	15
TGGACCG	125	2.5826248E-6	9.876377	5
AACGCAT	125	2.5826248E-6	9.876377	6
CTAAGCT	155	4.087451E-8	9.802855	4
GTCTATA	90	0.0011114406	9.502243	1
CTAGATC	120	1.7081069E-5	9.496516	4
>>END_MODULE