FastQCFastQC Report
Mon 15 Aug 2016
SRR2921178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2073858
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63310.30527644612119054No Hit
GGTATCAACGCAGAGTACTTTTTTT43030.20748768719941285No Hit
TATCAACGCAGAGTACTTTTTTTTT42310.20401589694183497No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29590.1426809357246253No Hit
GGTATCAACGCAGAGTACATGGGGG26340.1270096602563917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19400.013.08941651
CCCCTAT604.054498E-412.6808841
TGGACCG1459.822543E-1111.7929545
GGACCGT1354.774847E-911.2526016
CGATTGG700.001491323710.85752914
ACCGTCT1053.473977E-610.8554338
ACATCGT700.001496433710.85307815
CCCTTAT1158.693514E-710.7511841
TGCTACG803.7705788E-410.687880510
TACGTAA856.592767E-410.05918113
ATTACGA856.5956917E-410.0586965
ACCCCGC856.6044735E-410.057248
CAACGGT856.6308817E-410.05287556
CTATACC1054.0870284E-59.9565784
GGCGATT1054.1044033E-59.95273512
GTCCCTA1151.0130716E-59.924171
GTATCAA38850.09.81666851
CTATTAC800.00447491849.5106631
CCCTACG1401.0426702E-69.5106621
GATCTAG900.00110247359.5106621
Produced by FastQC (version 0.11.5)