##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921178_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2073858
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.402418101914403	31.0	30.0	33.0	25.0	34.0
2	29.65472804791842	31.0	30.0	34.0	26.0	34.0
3	29.776699754756592	31.0	30.0	34.0	26.0	34.0
4	33.47142186205613	35.0	35.0	37.0	28.0	37.0
5	33.003206584057345	35.0	33.0	37.0	26.0	37.0
6	32.957934921291624	35.0	33.0	37.0	26.0	37.0
7	32.7969166644968	35.0	33.0	37.0	26.0	37.0
8	32.73132200951078	36.0	33.0	37.0	25.0	37.0
9	34.079164050769144	38.0	34.0	39.0	25.0	39.0
10	33.94579908556902	38.0	34.0	39.0	25.0	39.0
11	33.97223098206338	38.0	34.0	39.0	25.0	39.0
12	33.836864915534235	38.0	34.0	39.0	24.0	39.0
13	33.81440484353316	38.0	34.0	39.0	23.0	39.0
14	34.71419788625836	39.0	34.0	41.0	23.0	41.0
15	34.70618046172881	39.0	34.0	41.0	23.0	41.0
16	34.6800856182053	39.0	34.0	41.0	23.0	41.0
17	34.66519790651047	39.0	34.0	41.0	22.0	41.0
18	34.614233954301596	39.0	34.0	41.0	22.0	41.0
19	34.65540890456338	39.0	34.0	41.0	22.0	41.0
20	34.58590366360667	39.0	34.0	41.0	20.0	41.0
21	34.51578748400325	39.0	34.0	41.0	19.0	41.0
22	34.42082726975521	39.0	34.0	41.0	18.0	41.0
23	34.28232164400841	39.0	34.0	41.0	18.0	41.0
24	34.19150780815273	39.0	34.0	41.0	17.0	41.0
25	34.055816261286935	39.0	34.0	41.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	71736.0
3	6.0
4	1150.0
5	9836.0
6	10918.0
7	8234.0
8	6622.0
9	5687.0
10	4943.0
11	4642.0
12	4209.0
13	4099.0
14	3975.0
15	3834.0
16	4048.0
17	4294.0
18	4718.0
19	5648.0
20	7201.0
21	9006.0
22	11362.0
23	13710.0
24	16262.0
25	18626.0
26	21240.0
27	23737.0
28	27595.0
29	33186.0
30	42298.0
31	53987.0
32	68601.0
33	87771.0
34	114885.0
35	151403.0
36	204413.0
37	305726.0
38	704250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.27627749380982	19.037083158367242	9.294005782327702	31.392633565495238
2	15.160536545896585	21.186792924105703	34.50221760602703	29.150452923970686
3	17.889532755091853	24.100861187795665	28.040193587568563	29.969412469543915
4	13.514600991153722	16.767885538727555	32.39587675420314	37.32163671591559
5	14.660766790859341	37.52900965267545	32.28445044017474	15.52577311629047
6	34.161962400913865	34.78617068829753	16.095412008812694	14.956454901975913
7	29.663568896023207	32.25612952098433	19.380121194470014	18.70018038852245
8	27.805485382423612	34.55480391679392	18.492612010864875	19.147098689917588
9	27.111885191753725	15.67571164467384	18.956264122230163	38.256139041342266
10	17.17658586074842	28.739335094302504	30.585941756860883	23.49813728808819
11	35.450958797366056	22.63950877788536	21.55577457942916	20.353757845319425
12	23.49047041793604	25.45622699336213	28.236504138663303	22.81679845003853
13	28.916858258239724	21.30514365510431	24.90163251450438	24.876365572151588
14	22.958653987171274	21.143663211195417	25.468775327472155	30.428907474161154
15	24.97347357292645	28.147047078138172	22.530052030380958	24.349427318554415
16	24.587278679291774	27.188036590758184	24.15026686989508	24.07441786005496
17	23.190690973056014	27.48394538102416	25.5814525391806	23.743911106739226
18	23.445807346943496	26.698899916194197	26.62242350188684	23.23286923497547
19	24.56088747358767	26.056903081334067	26.527575389060498	22.854634056017765
20	24.854835769855026	25.543745039438576	26.24697544383463	23.354443746871773
21	25.08681078792514	25.187593760714215	26.104670591576763	23.62092485978388
22	24.963232776786068	24.99751670557965	25.745205312996355	24.294045204637925
23	24.613196197419782	25.12287503224671	25.982915874060623	24.281012896272884
24	24.329275319829552	25.696015939520862	26.011662428954224	23.96304631169536
25	24.19423931274724	25.37460983927044	26.201125169695466	24.23002567828685
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1240.0
1	1240.0
2	1367.5
3	1495.0
4	1495.0
5	1495.0
6	1878.5
7	2262.0
8	2262.0
9	2262.0
10	2803.0
11	3344.0
12	3344.0
13	3344.0
14	4239.0
15	5134.0
16	5134.0
17	5134.0
18	9937.5
19	14741.0
20	14741.0
21	14741.0
22	23061.0
23	31381.0
24	31381.0
25	31381.0
26	46501.5
27	61622.0
28	61622.0
29	61622.0
30	79079.5
31	96537.0
32	96537.0
33	96537.0
34	115997.0
35	135457.0
36	135457.0
37	135457.0
38	158727.0
39	181997.0
40	181997.0
41	181997.0
42	204598.5
43	227200.0
44	227200.0
45	227200.0
46	248698.0
47	270196.0
48	270196.0
49	270196.0
50	279942.5
51	289689.0
52	289689.0
53	289689.0
54	274445.5
55	259202.0
56	259202.0
57	259202.0
58	230114.0
59	201026.0
60	201026.0
61	201026.0
62	170621.0
63	140216.0
64	140216.0
65	140216.0
66	111938.5
67	83661.0
68	83661.0
69	83661.0
70	61835.0
71	40009.0
72	40009.0
73	40009.0
74	28556.5
75	17104.0
76	17104.0
77	17104.0
78	11944.5
79	6785.0
80	6785.0
81	6785.0
82	4597.0
83	2409.0
84	2409.0
85	2409.0
86	1625.0
87	841.0
88	841.0
89	841.0
90	547.5
91	254.0
92	254.0
93	254.0
94	152.5
95	51.0
96	51.0
97	51.0
98	28.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09778875892177767
2	0.0
3	0.03814147352422393
4	0.054102064847255694
5	0.06133496121720967
6	0.0014947985831238204
7	4.821930913302646E-5
8	9.161668735275029E-4
9	0.0
10	0.0
11	4.8219309133026465E-4
12	0.0
13	9.643861826605292E-5
14	0.048701502224356735
15	0.021891566346394015
16	4.821930913302646E-5
17	0.0
18	7.715089461284234E-4
19	7.715089461284234E-4
20	0.0
21	0.004580834367637515
22	0.0
23	1.446579273990794E-4
24	9.161668735275029E-4
25	0.15608590366360667
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2073858.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.823791738500525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.91141225842529	20.264311309884242
2	14.010573042850478	9.477814094768483
3	7.48898847430927	7.599179594612025
4	4.6255899994801934	6.258199712404351
5	3.079638225019425	5.2082520976491224
6	2.202113706942258	4.469030124486738
7	1.6103119681046816	3.8126799650231358
8	1.2046607492885042	3.2596955439584283
9	0.9348547739904656	2.8458299863076832
>10	4.667634736772594	26.83038601609012
>50	0.19792639428168335	4.532013007069333
>100	0.06226143546586451	3.3786088050107717
>500	0.0018730377107313576	0.4622349670589174
>1k	0.0020171175346337697	1.2932345093139157
>5k	1.4407982390241212E-4	0.30853026636267855
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6331	0.30527644612119054	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4303	0.20748768719941285	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4231	0.20401589694183497	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2959	0.1426809357246253	No Hit
GGTATCAACGCAGAGTACATGGGGG	2634	0.1270096602563917	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	4.821930913302646E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1940	0.0	13.0894165	1
CCCCTAT	60	4.054498E-4	12.680884	1
TGGACCG	145	9.822543E-11	11.792954	5
GGACCGT	135	4.774847E-9	11.252601	6
CGATTGG	70	0.0014913237	10.857529	14
ACCGTCT	105	3.473977E-6	10.855433	8
ACATCGT	70	0.0014964337	10.853078	15
CCCTTAT	115	8.693514E-7	10.751184	1
TGCTACG	80	3.7705788E-4	10.6878805	10
TACGTAA	85	6.592767E-4	10.059181	13
ATTACGA	85	6.5956917E-4	10.058696	5
ACCCCGC	85	6.6044735E-4	10.05724	8
CAACGGT	85	6.6308817E-4	10.0528755	6
CTATACC	105	4.0870284E-5	9.956578	4
GGCGATT	105	4.1044033E-5	9.952735	12
GTCCCTA	115	1.0130716E-5	9.92417	1
GTATCAA	3885	0.0	9.8166685	1
CTATTAC	80	0.0044749184	9.510663	1
CCCTACG	140	1.0426702E-6	9.510662	1
GATCTAG	90	0.0011024735	9.510662	1
>>END_MODULE