FastQCFastQC Report
Mon 15 Aug 2016
SRR2921177_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921177_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520109
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31260.12404225372791415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14700.013.1887861
TCGCACT951.0464846E-611.99441054
TCGTCGC700.001493312210.85596413
GTACGGG909.482188E-510.5596491
GTATTAA2950.010.3090811
GTATCAA31100.010.2676461
GCGTAGG750.00264004210.1372631
TAGGGTG2500.09.875795
GTCTAGA3100.09.8102551
AGACCGA1554.0943632E-89.8020796
CTCGCAC1201.7093707E-59.49614053
TCTATAC1901.0604708E-99.49614053
TATACTA1752.6897396E-89.2246395
TCTCGCA1356.845805E-69.1486062
CTAGCAC2700.09.1444313
GTCTAAG3550.09.102121
GTCTCGC1151.1063341E-49.090481
TCGATTC950.00182608058.999022513
ACCCGGC850.00746469348.9371896
TCTAGCA1502.7009228E-68.8671112
Produced by FastQC (version 0.11.5)