##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921177_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2520109
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.003750631421102	31.0	27.0	31.0	10.0	34.0
2	26.594808795968746	31.0	26.0	31.0	10.0	34.0
3	26.465386219405588	31.0	26.0	31.0	10.0	34.0
4	30.336302517073666	35.0	30.0	37.0	10.0	37.0
5	30.00336771147597	35.0	30.0	37.0	10.0	37.0
6	30.345924323114595	35.0	31.0	37.0	8.0	37.0
7	30.20131787950442	35.0	30.0	37.0	7.0	37.0
8	29.874491936658295	35.0	30.0	37.0	2.0	37.0
9	31.430338132199836	37.0	31.0	39.0	2.0	39.0
10	30.777238206760103	37.0	30.0	39.0	2.0	39.0
11	31.272160053394515	37.0	30.0	39.0	2.0	39.0
12	30.982340843193686	37.0	30.0	39.0	2.0	39.0
13	30.988765565298962	37.0	30.0	39.0	2.0	39.0
14	32.05847326445007	38.0	31.0	40.0	2.0	41.0
15	31.71368420969093	38.0	30.0	40.0	2.0	41.0
16	31.632581368504297	38.0	30.0	40.0	2.0	41.0
17	31.943554028813832	38.0	31.0	40.0	2.0	41.0
18	31.999982937245967	38.0	31.0	40.0	2.0	41.0
19	31.700162969141413	38.0	30.0	40.0	2.0	41.0
20	31.567846073324606	38.0	30.0	40.0	2.0	41.0
21	31.511247330968622	38.0	30.0	40.0	2.0	41.0
22	30.737181209225476	38.0	28.0	40.0	2.0	41.0
23	30.8265408361305	38.0	30.0	40.0	2.0	41.0
24	30.45792106611262	37.0	29.0	40.0	2.0	41.0
25	29.962164334955354	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	219108.0
3	4.0
4	1935.0
5	10665.0
6	12604.0
7	10604.0
8	9160.0
9	8176.0
10	7872.0
11	7460.0
12	7239.0
13	7264.0
14	6897.0
15	7216.0
16	7631.0
17	8322.0
18	9748.0
19	12041.0
20	15075.0
21	19197.0
22	22843.0
23	26944.0
24	30979.0
25	34967.0
26	38998.0
27	43301.0
28	48485.0
29	56560.0
30	67013.0
31	79611.0
32	99334.0
33	124551.0
34	157999.0
35	201726.0
36	264155.0
37	342715.0
38	491710.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.49562806737873	17.812549121723432	12.158728066902398	25.533094743995438
2	16.213362957151602	19.779457131354583	38.484230319363924	25.52294959212989
3	18.802857761970113	23.103827626249437	30.240850819967502	27.852463791812948
4	13.620381342580504	15.685715805088748	34.88563135508132	35.80827149724942
5	14.102298012637938	36.72768429202583	33.93091635967368	15.23910133566255
6	33.90036109678187	34.25697392960597	16.991944764096665	14.850720209515494
7	29.668990065535322	31.501320428648246	20.29452205641633	18.535167449400102
8	26.113399844607738	34.51680387163234	20.51308810773017	18.856708176029752
9	26.96597332867713	15.8044949572112	19.15902592725606	38.07050578685561
10	16.113351391376742	27.9698748661718	32.68296864074996	23.233805101701492
11	35.26507280632549	22.705247484360093	22.177752837883098	19.851926871431317
12	23.213492732774444	24.780237114936256	29.269626469566944	22.736643682722356
13	28.450239195525228	21.279175207001852	25.24460247509331	25.025983122379614
14	22.74046866411479	21.021504416174054	25.79803668272007	30.439990236991086
15	24.713009039897777	28.01995285602038	22.990785494892734	24.276252609189108
16	24.189615568272345	27.16155770048295	24.935409388769788	23.713417342474923
17	23.136733245366244	27.397142816312336	25.752801370635186	23.713322567686227
18	23.069482745208237	26.8730065742368	26.51829784261522	23.539212837939743
19	24.293117126756517	26.30614333753094	26.72012299930666	22.680616536405882
20	24.760308196153975	25.81174539448044	26.266522839678153	23.161423569687432
21	25.201288180853354	25.496780738568663	25.78882026035097	23.513110820227013
22	24.342968890130525	24.962578949289913	26.94529939221547	23.749152768364095
23	24.683128594407382	25.331773896471542	25.866041092775248	24.11905641634583
24	24.157389207232114	25.51542433438619	26.288218549204167	24.03896790917753
25	23.902935882588615	25.260071478545477	26.79707946556532	24.03991317330059
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1286.0
1	1286.0
2	1426.0
3	1566.0
4	1566.0
5	1566.0
6	2141.0
7	2716.0
8	2716.0
9	2716.0
10	3353.0
11	3990.0
12	3990.0
13	3990.0
14	5918.0
15	7846.0
16	7846.0
17	7846.0
18	13931.0
19	20016.0
20	20016.0
21	20016.0
22	30793.0
23	41570.0
24	41570.0
25	41570.0
26	59893.0
27	78216.0
28	78216.0
29	78216.0
30	100729.5
31	123243.0
32	123243.0
33	123243.0
34	149505.0
35	175767.0
36	175767.0
37	175767.0
38	205982.5
39	236198.0
40	236198.0
41	236198.0
42	265474.5
43	294751.0
44	294751.0
45	294751.0
46	316021.5
47	337292.0
48	337292.0
49	337292.0
50	343510.0
51	349728.0
52	349728.0
53	349728.0
54	325049.0
55	300370.0
56	300370.0
57	300370.0
58	265683.5
59	230997.0
60	230997.0
61	230997.0
62	193255.0
63	155513.0
64	155513.0
65	155513.0
66	121735.0
67	87957.0
68	87957.0
69	87957.0
70	65440.0
71	42923.0
72	42923.0
73	42923.0
74	30309.5
75	17696.0
76	17696.0
77	17696.0
78	12306.0
79	6916.0
80	6916.0
81	6916.0
82	4676.5
83	2437.0
84	2437.0
85	2437.0
86	1650.5
87	864.0
88	864.0
89	864.0
90	538.5
91	213.0
92	213.0
93	213.0
94	123.0
95	33.0
96	33.0
97	33.0
98	19.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034006465593353304
2	0.056148365011195946
3	0.014880308748550163
4	0.010912226415603452
5	0.009364674305754236
6	3.5712740996520387E-4
7	0.003729997392969907
8	0.0013888288165313485
9	0.006904463259327275
10	0.0034522316296636377
11	0.010832864768944519
12	0.009285312659095301
13	0.008094887959211287
14	0.015634244391810038
15	0.006706059142679939
16	0.030951042196984337
17	0.03908561097952509
18	0.022895835061102515
19	0.016665945798376183
20	0.016467541681728846
21	0.02329264329439719
22	0.005436272796136993
23	0.01571360603846897
24	0.011586800412204393
25	0.4628371233149043
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2520109.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.863761371733496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.308580810504	34.13096895670938
2	11.963573192694984	11.45243224896841
3	5.7232644536702075	8.218108922333865
4	3.249604816556658	6.221532379684147
5	2.0046780624559517	4.797571620427036
6	1.3513306720490856	3.8807861292757013
7	0.8954094264568819	3.000036418255307
8	0.6762325009765137	2.589362484684031
9	0.48902889417446194	2.106608606518424
>10	2.218015671445241	17.89437923754558
>50	0.09308248668406509	3.0138674604040263
>100	0.025738762665091293	1.9144625316658022
>500	9.448674314496222E-4	0.3221658081951484
>1k	5.153822353351697E-4	0.45771719533329575
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3126	0.12404225372791415	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.936164665893419E-5
2	0.0	0.0	0.0	7.936164665893419E-5	7.936164665893419E-5
3	0.0	0.0	0.0	1.190424699884013E-4	7.936164665893419E-5
4	0.0	0.0	0.0	1.190424699884013E-4	7.936164665893419E-5
5	0.0	0.0	0.0	1.190424699884013E-4	7.936164665893419E-5
6	0.0	0.0	0.0	1.190424699884013E-4	1.190424699884013E-4
7	0.0	0.0	0.0	1.190424699884013E-4	1.190424699884013E-4
8	0.0	0.0	0.0	1.5872329331786838E-4	1.190424699884013E-4
9	0.0	0.0	0.0	2.7776576330626967E-4	1.190424699884013E-4
10	0.0	0.0	0.0	2.7776576330626967E-4	1.5872329331786838E-4
11	0.0	0.0	0.0	2.7776576330626967E-4	1.5872329331786838E-4
12	0.0	0.0	0.0	3.1744658663573677E-4	2.7776576330626967E-4
13	0.0	0.0	0.0	3.5712740996520387E-4	2.7776576330626967E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1470	0.0	13.188786	1
TCGCACT	95	1.0464846E-6	11.9944105	4
TCGTCGC	70	0.0014933122	10.855964	13
GTACGGG	90	9.482188E-5	10.559649	1
GTATTAA	295	0.0	10.309081	1
GTATCAA	3110	0.0	10.267646	1
GCGTAGG	75	0.002640042	10.137263	1
TAGGGTG	250	0.0	9.87579	5
GTCTAGA	310	0.0	9.810255	1
AGACCGA	155	4.0943632E-8	9.802079	6
CTCGCAC	120	1.7093707E-5	9.4961405	3
TCTATAC	190	1.0604708E-9	9.4961405	3
TATACTA	175	2.6897396E-8	9.224639	5
TCTCGCA	135	6.845805E-6	9.148606	2
CTAGCAC	270	0.0	9.144431	3
GTCTAAG	355	0.0	9.10212	1
GTCTCGC	115	1.1063341E-4	9.09048	1
TCGATTC	95	0.0018260805	8.9990225	13
ACCCGGC	85	0.0074646934	8.937189	6
TCTAGCA	150	2.7009228E-6	8.867111	2
>>END_MODULE