FastQCFastQC Report
Mon 15 Aug 2016
SRR2921177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520109
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58040.23030749860422703No Hit
GGTATCAACGCAGAGTACTTTTTTT38820.15404095616499128No Hit
TATCAACGCAGAGTACTTTTTTTTT37830.15011255465537401No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25890.10273365159999032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.015.0374061
ACGGTCC400.00528744914.2470978
TCGATCG500.001490209713.31156119
CCCTATA1001.4371835E-712.3543512
TGTCGTA550.003067193312.09204714
GTATCAA33400.010.7659761
CCCCTAT1659.094947E-1010.3775931
GTAAGAC1106.0235016E-610.3672883
GTACGGG856.514861E-410.072371
TAGACAG2002.5465852E-119.9749485
GTATTAG1856.330083E-109.76989651
AATCGGT800.0044848349.50825719
TATTAAG2002.6375346E-109.5033462
CTAGGTT1002.7466283E-49.5033464
GCACCGT1502.6768066E-79.4933536
GCTAGTC1356.841994E-69.14900813
GACTGCG1356.911243E-69.1417477
GCTCCGT1151.1195177E-49.0805986
TCGTTAG950.00182271629.00084614
CGTTAGG950.00182997428.99691615
Produced by FastQC (version 0.11.5)