##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921177_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2520109
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.495569040862915	31.0	30.0	33.0	26.0	34.0
2	29.75630895330321	31.0	30.0	34.0	26.0	34.0
3	29.88844530137387	31.0	30.0	34.0	26.0	34.0
4	33.58145699253485	35.0	35.0	37.0	29.0	37.0
5	33.11570967763696	35.0	35.0	37.0	27.0	37.0
6	33.071432227733006	36.0	35.0	37.0	26.0	37.0
7	32.91391364421142	36.0	33.0	37.0	26.0	37.0
8	32.84969142207738	36.0	33.0	37.0	26.0	37.0
9	34.22035356407203	38.0	35.0	39.0	26.0	39.0
10	34.085636772060255	38.0	34.0	39.0	25.0	39.0
11	34.11678304390802	38.0	34.0	39.0	25.0	39.0
12	33.98265075042389	38.0	34.0	39.0	25.0	39.0
13	33.96216909665415	38.0	34.0	39.0	25.0	39.0
14	34.88233207373173	39.0	34.0	41.0	25.0	41.0
15	34.87222100313915	39.0	34.0	41.0	25.0	41.0
16	34.852563520069964	39.0	34.0	41.0	24.0	41.0
17	34.824854401139	39.0	34.0	41.0	24.0	41.0
18	34.77975873265799	39.0	34.0	41.0	24.0	41.0
19	34.821658507628044	39.0	34.0	41.0	23.0	41.0
20	34.751352421661124	39.0	34.0	41.0	23.0	41.0
21	34.67917578168246	39.0	34.0	41.0	22.0	41.0
22	34.586631371897006	39.0	34.0	41.0	21.0	41.0
23	34.45544418912039	39.0	34.0	41.0	18.0	41.0
24	34.37383700466924	39.0	34.0	41.0	18.0	41.0
25	34.236453264521494	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81726.0
3	7.0
4	1308.0
5	11470.0
6	12704.0
7	9812.0
8	7822.0
9	6620.0
10	5826.0
11	5388.0
12	4990.0
13	4802.0
14	4557.0
15	4626.0
16	4848.0
17	5000.0
18	5557.0
19	6529.0
20	8270.0
21	10648.0
22	13470.0
23	16418.0
24	19400.0
25	22389.0
26	25650.0
27	28585.0
28	33298.0
29	40163.0
30	50909.0
31	64939.0
32	82618.0
33	106530.0
34	138379.0
35	183209.0
36	248009.0
37	371625.0
38	872008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.99964251597258	18.905860553424993	9.214349408860441	31.880147521741982
2	15.043396932434272	21.00952776248964	34.90091103202282	29.04616427305327
3	17.85025270689335	23.513292716039384	28.225684801542123	30.410769775525143
4	13.394350953617026	16.46312461141069	33.02467199687461	37.11785243809767
5	14.769250316652968	37.24541696942264	32.465168173498036	15.520164540426359
6	33.92741282956154	35.12631266079016	16.2029256616231	14.743348848025201
7	29.79730882627208	31.893067311088096	19.432440904888317	18.877182957751508
8	27.348268548151882	34.842013575423756	18.72694191853555	19.082775957888806
9	26.98569784084736	15.49996448566312	18.988980238553175	38.525357434936346
10	16.799392407233178	28.443610970795312	31.00592077564899	23.751075846322518
11	35.54618070533071	22.59540845744472	21.777465782480107	20.080945054744458
12	23.394146840473965	24.87765410146942	28.681656229948782	23.04654282810783
13	28.66016788475839	21.034760255898465	25.003591181486804	25.301480677856336
14	22.885873559161478	20.845305168140342	25.56667787589047	30.702143396807713
15	24.886337792768245	28.089756529697834	22.44968996164756	24.574215715886353
16	24.781846450059405	27.041000565869872	24.10887399831906	24.068278985751668
17	23.25190031538331	27.297242816577704	25.620925769847958	23.82993109819103
18	23.226250132937984	26.534977372764455	26.544620337051366	23.694152157246194
19	24.42719586986585	26.049725894488724	26.423850651682628	23.099227583962794
20	24.788600162056674	25.581622360329288	26.18560489122283	23.444172586391208
21	25.073378723107037	25.214223010996093	25.951542262810918	23.760856003085955
22	25.00181540343026	25.089311896626366	25.60909265144532	24.299780048498057
23	24.547297594912447	25.272526193109375	25.71553626480288	24.464639947175296
24	24.265260283975014	25.469773089835474	26.00149209901823	24.263474527171283
25	24.229606482235937	25.29907524955066	26.082803838910745	24.388514429302656
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	959.0
1	959.0
2	1067.5
3	1176.0
4	1176.0
5	1176.0
6	1592.5
7	2009.0
8	2009.0
9	2009.0
10	2625.5
11	3242.0
12	3242.0
13	3242.0
14	4384.0
15	5526.0
16	5526.0
17	5526.0
18	11147.0
19	16768.0
20	16768.0
21	16768.0
22	26893.0
23	37018.0
24	37018.0
25	37018.0
26	54886.0
27	72754.0
28	72754.0
29	72754.0
30	93318.5
31	113883.0
32	113883.0
33	113883.0
34	138416.0
35	162949.0
36	162949.0
37	162949.0
38	192713.5
39	222478.0
40	222478.0
41	222478.0
42	252635.0
43	282792.0
44	282792.0
45	282792.0
46	308056.5
47	333321.0
48	333321.0
49	333321.0
50	345914.5
51	358508.0
52	358508.0
53	358508.0
54	336219.5
55	313931.0
56	313931.0
57	313931.0
58	280453.0
59	246975.0
60	246975.0
61	246975.0
62	208083.5
63	169192.0
64	169192.0
65	169192.0
66	133349.5
67	97507.0
68	97507.0
69	97507.0
70	72621.0
71	47735.0
72	47735.0
73	47735.0
74	33784.5
75	19834.0
76	19834.0
77	19834.0
78	13703.0
79	7572.0
80	7572.0
81	7572.0
82	5135.5
83	2699.0
84	2699.0
85	2699.0
86	1827.5
87	956.0
88	956.0
89	956.0
90	616.5
91	277.0
92	277.0
93	277.0
94	158.5
95	40.0
96	40.0
97	40.0
98	24.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09975758985028028
2	0.0
3	0.03908561097952509
4	0.054878578664653004
5	0.06265602003722855
6	0.005237868679489657
7	7.539356432598749E-4
8	0.004523613859559249
9	0.0
10	0.0
11	0.005079145386171788
12	0.0
13	0.0018649986964849536
14	0.05356911149478059
15	0.023094239177749852
16	0.003769678216299374
17	3.9680823329467096E-5
18	0.005436272796136993
19	0.003729997392969907
20	1.190424699884013E-4
21	0.012102651115487466
22	3.1744658663573677E-4
23	0.0030157425730394996
24	0.006706059142679939
25	0.16614360728047875
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2520109.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.09372095883444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.44621690690391	21.212826691480668
2	14.608633452002511	10.25342611908941
3	7.443651332892627	7.836762683741698
4	4.634170544838623	6.505211519048657
5	2.9141551392136917	5.113427364315932
6	2.059183024681619	4.335863668280726
7	1.5245214035931396	3.7450790113427788
8	1.0914116464993981	3.0641356618777547
9	0.8581929357910795	2.7105465075745556
>10	4.149986157989403	25.154208631851727
>50	0.19753196597281364	4.729556387909653
>100	0.06972066771422751	3.8892695614113966
>500	0.0013694722993082742	0.364614279985994
>1k	0.0011412269160900657	0.8526222986183039
>5k	1.1412269160900659E-4	0.23244961347083634
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5804	0.23030749860422703	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3882	0.15404095616499128	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3783	0.15011255465537401	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2589	0.10273365159999032	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.190424699884013E-4
2	0.0	0.0	0.0	0.0	1.190424699884013E-4
3	0.0	0.0	0.0	0.0	1.190424699884013E-4
4	0.0	0.0	0.0	0.0	1.190424699884013E-4
5	0.0	0.0	0.0	0.0	1.190424699884013E-4
6	0.0	0.0	0.0	0.0	1.5872329331786838E-4
7	0.0	0.0	0.0	0.0	1.5872329331786838E-4
8	0.0	0.0	0.0	1.5872329331786838E-4	1.5872329331786838E-4
9	0.0	0.0	0.0	1.984041166473355E-4	1.5872329331786838E-4
10	0.0	0.0	0.0	1.984041166473355E-4	1.5872329331786838E-4
11	0.0	0.0	0.0	1.984041166473355E-4	1.5872329331786838E-4
12	0.0	0.0	0.0	2.380849399768026E-4	2.7776576330626967E-4
13	0.0	0.0	0.0	2.380849399768026E-4	3.5712740996520387E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1455	0.0	15.037406	1
ACGGTCC	40	0.005287449	14.247097	8
TCGATCG	50	0.0014902097	13.311561	19
CCCTATA	100	1.4371835E-7	12.354351	2
TGTCGTA	55	0.0030671933	12.092047	14
GTATCAA	3340	0.0	10.765976	1
CCCCTAT	165	9.094947E-10	10.377593	1
GTAAGAC	110	6.0235016E-6	10.367288	3
GTACGGG	85	6.514861E-4	10.07237	1
TAGACAG	200	2.5465852E-11	9.974948	5
GTATTAG	185	6.330083E-10	9.7698965	1
AATCGGT	80	0.004484834	9.508257	19
TATTAAG	200	2.6375346E-10	9.503346	2
CTAGGTT	100	2.7466283E-4	9.503346	4
GCACCGT	150	2.6768066E-7	9.493353	6
GCTAGTC	135	6.841994E-6	9.149008	13
GACTGCG	135	6.911243E-6	9.141747	7
GCTCCGT	115	1.1195177E-4	9.080598	6
TCGTTAG	95	0.0018227162	9.000846	14
CGTTAGG	95	0.0018299742	8.996916	15
>>END_MODULE