FastQCFastQC Report
Mon 15 Aug 2016
SRR2921176_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921176_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502956
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT1201.2691453E-711.087871
GTCTTAC1900.011.0045031
GGTATCA13400.010.7096061
TAGACCG803.7853356E-410.6835435
GTCCTAA2050.010.66291
ACCGAAC909.553339E-510.551868
GTATCAA26850.010.33571
GTCGTAC750.002639559310.1374821
AATCGGA750.002651843710.13180916
GGCTTAG1752.557499E-99.7754291
GTATAAA2750.09.6766871
ACGCTCT900.00111758739.4966749
AACCGTC800.00453163789.4962947
GCACCGT1201.7090293E-59.4962946
GAACCGT1701.6763806E-89.4962946
GTGTAAG3050.09.3480871
GTATTAG2151.05501385E-109.2828681
CAGGACT4150.09.1532374
CCTAGAC2201.6916601E-109.0648253
TAATACC1804.246067E-88.9689024
Produced by FastQC (version 0.11.5)