##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921176_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2502956
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.8587130576806	31.0	27.0	31.0	10.0	34.0
2	26.639146672973876	31.0	26.0	31.0	10.0	34.0
3	26.451881295556134	31.0	26.0	31.0	10.0	34.0
4	30.258201502543393	35.0	30.0	37.0	10.0	37.0
5	29.99363632441002	35.0	30.0	37.0	10.0	37.0
6	30.319150636287652	35.0	31.0	37.0	8.0	37.0
7	30.247990775706803	35.0	30.0	37.0	2.0	37.0
8	29.901321077957423	35.0	30.0	37.0	2.0	37.0
9	31.489819637260904	37.0	31.0	39.0	2.0	39.0
10	30.824197868440358	37.0	30.0	39.0	2.0	39.0
11	31.266940769234456	37.0	30.0	39.0	2.0	39.0
12	30.970594369217835	37.0	30.0	39.0	2.0	39.0
13	30.975168960221435	37.0	30.0	39.0	2.0	39.0
14	32.05044834987111	38.0	31.0	40.0	2.0	41.0
15	31.706215770472994	38.0	30.0	40.0	2.0	41.0
16	31.62339609645555	38.0	30.0	40.0	2.0	41.0
17	31.935365224158954	38.0	31.0	40.0	2.0	41.0
18	31.987283436065198	38.0	31.0	40.0	2.0	41.0
19	31.698180471410605	38.0	30.0	40.0	2.0	41.0
20	31.549311693853188	38.0	30.0	40.0	2.0	41.0
21	31.49840508582652	38.0	30.0	40.0	2.0	41.0
22	30.76060386199358	38.0	29.0	40.0	2.0	41.0
23	30.846492707023216	38.0	30.0	40.0	2.0	41.0
24	30.464734497929648	37.0	29.0	40.0	2.0	41.0
25	29.986366520226483	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	219377.0
3	8.0
4	1776.0
5	10244.0
6	12032.0
7	10589.0
8	8865.0
9	8365.0
10	7847.0
11	7443.0
12	7320.0
13	6838.0
14	7073.0
15	7130.0
16	7589.0
17	8482.0
18	9646.0
19	11880.0
20	14864.0
21	18681.0
22	23092.0
23	27074.0
24	30682.0
25	34644.0
26	38516.0
27	43233.0
28	48133.0
29	56017.0
30	66717.0
31	78149.0
32	97300.0
33	121835.0
34	155761.0
35	199107.0
36	261039.0
37	344112.0
38	491496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.552123916299905	17.89613904613609	12.103908914037783	25.44782812352622
2	15.584556793928956	19.597861990366887	39.372292460082214	25.44528875562195
3	18.82941557489294	23.08922115945191	30.052997327956483	28.02836593769867
4	13.27851232074288	15.582673043804865	35.84407177876604	35.294742856686206
5	13.734651205627795	36.71671752464618	35.08844973131244	14.460181538413591
6	33.91859173611832	34.77430541682981	16.831885702413963	14.475217144637906
7	29.2311890551597	31.595946746105884	20.473391032243683	18.699473166490733
8	26.31263104735604	34.46509505727728	20.734408560908253	18.487865334458423
9	26.483311657566837	15.772925868878593	19.321278810290096	38.42248366326447
10	15.408338900271367	28.260813976903332	33.42564062405109	22.90520649877421
11	35.40994309747749	22.66447330304339	22.488023938936376	19.43755966054275
12	22.946392396819167	24.782498555584233	29.877989820764583	22.393119226832013
13	28.45041519614287	21.369845608486198	25.58389766952361	24.595841525847323
14	22.15385771106666	21.007329183512493	26.290438975816333	30.548374129604518
15	24.32642682681739	28.36820548431782	23.09373188285642	24.21163580600837
16	23.82323367154292	27.596759006888348	25.08688368137428	23.49312364019445
17	22.805824808112586	27.595456093314596	26.082739005942475	23.51598009263034
18	22.778503750414604	27.494075663665534	26.715180287645012	23.01224029827485
19	24.010969711815296	26.60526739571641	26.989359155765353	22.39440373670294
20	24.332022405910124	26.388921078226485	26.381488671173532	22.897567844689863
21	24.650513549868005	26.004541265815906	26.232923298004383	23.112021886311705
22	23.776315700101204	25.57108330526512	27.313270452539545	23.33933054209413
23	23.970935698990125	25.755407699786105	26.409010828386126	23.86464577283765
24	23.550038998069276	26.04943165108875	26.65757777237914	23.742951578462836
25	23.444152880959006	25.760858264419852	27.103763015486315	23.69122583913483
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1092.0
1	1092.0
2	1192.5
3	1293.0
4	1293.0
5	1293.0
6	1675.0
7	2057.0
8	2057.0
9	2057.0
10	2775.5
11	3494.0
12	3494.0
13	3494.0
14	5459.5
15	7425.0
16	7425.0
17	7425.0
18	13573.0
19	19721.0
20	19721.0
21	19721.0
22	31180.5
23	42640.0
24	42640.0
25	42640.0
26	63226.0
27	83812.0
28	83812.0
29	83812.0
30	108112.0
31	132412.0
32	132412.0
33	132412.0
34	159780.5
35	187149.0
36	187149.0
37	187149.0
38	217156.5
39	247164.0
40	247164.0
41	247164.0
42	274149.0
43	301134.0
44	301134.0
45	301134.0
46	321780.0
47	342426.0
48	342426.0
49	342426.0
50	342766.5
51	343107.0
52	343107.0
53	343107.0
54	317434.5
55	291762.0
56	291762.0
57	291762.0
58	254879.5
59	217997.0
60	217997.0
61	217997.0
62	179842.0
63	141687.0
64	141687.0
65	141687.0
66	110119.0
67	78551.0
68	78551.0
69	78551.0
70	57446.0
71	36341.0
72	36341.0
73	36341.0
74	25410.0
75	14479.0
76	14479.0
77	14479.0
78	9686.0
79	4893.0
80	4893.0
81	4893.0
82	3294.0
83	1695.0
84	1695.0
85	1695.0
86	1084.5
87	474.0
88	474.0
89	474.0
90	297.0
91	120.0
92	120.0
93	120.0
94	73.0
95	26.0
96	26.0
97	26.0
98	15.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03515842867393593
2	0.05501495032273839
3	0.01506219046599301
4	0.010627434121894272
5	0.009388898566335166
6	6.392441577079261E-4
7	0.00311631526882614
8	0.001757921433696797
9	0.006432394336936007
10	0.003356031827966612
11	0.010187953763470073
12	0.008549890609343512
13	0.006512299856649497
14	0.014303088028714848
15	0.005873055698941572
16	0.030364097491126493
17	0.03867427154132953
18	0.022613262078917888
19	0.017139733978543768
20	0.016061009462411645
21	0.02277307311834487
22	0.004994094982093173
23	0.014662662867425557
24	0.010707339641607763
25	0.4710030859511713
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2502956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.79937365078355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.21268650262196	35.46347183820181
2	12.372591471744023	12.322946114597572
3	5.6730210371775	8.47538683077474
4	3.192900742126396	6.360178283480661
5	2.03685753991008	5.071711485169892
6	1.345489874899453	4.020273181407833
7	0.9184488816834244	3.2016725326668003
8	0.6627410495018096	2.640327132628245
9	0.47693345178838237	2.1375888454950855
>10	2.0311951601365807	16.373437713936347
>50	0.058088180006553844	1.9394234090897382
>100	0.017724293372743255	1.376714847455531
>500	9.087478339297552E-4	0.32373833539545294
>1k	4.1306719724066E-4	0.2931294497003409
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.995275985674538E-5	0.0
5	0.0	0.0	0.0	3.995275985674538E-5	0.0
6	0.0	0.0	3.995275985674538E-5	3.995275985674538E-5	0.0
7	0.0	0.0	3.995275985674538E-5	3.995275985674538E-5	0.0
8	0.0	0.0	3.995275985674538E-5	7.990551971349076E-5	0.0
9	0.0	0.0	3.995275985674538E-5	7.990551971349076E-5	0.0
10	0.0	0.0	3.995275985674538E-5	7.990551971349076E-5	0.0
11	0.0	0.0	3.995275985674538E-5	7.990551971349076E-5	0.0
12	3.995275985674538E-5	0.0	3.995275985674538E-5	7.990551971349076E-5	0.0
13	3.995275985674538E-5	0.0	3.995275985674538E-5	7.990551971349076E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAT	120	1.2691453E-7	11.08787	1
GTCTTAC	190	0.0	11.004503	1
GGTATCA	1340	0.0	10.709606	1
TAGACCG	80	3.7853356E-4	10.683543	5
GTCCTAA	205	0.0	10.6629	1
ACCGAAC	90	9.553339E-5	10.55186	8
GTATCAA	2685	0.0	10.3357	1
GTCGTAC	75	0.0026395593	10.137482	1
AATCGGA	75	0.0026518437	10.131809	16
GGCTTAG	175	2.557499E-9	9.775429	1
GTATAAA	275	0.0	9.676687	1
ACGCTCT	90	0.0011175873	9.496674	9
AACCGTC	80	0.0045316378	9.496294	7
GCACCGT	120	1.7090293E-5	9.496294	6
GAACCGT	170	1.6763806E-8	9.496294	6
GTGTAAG	305	0.0	9.348087	1
GTATTAG	215	1.05501385E-10	9.282868	1
CAGGACT	415	0.0	9.153237	4
CCTAGAC	220	1.6916601E-10	9.064825	3
TAATACC	180	4.246067E-8	8.968902	4
>>END_MODULE