FastQCFastQC Report
Mon 15 Aug 2016
SRR2921176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502956
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46900.18737844372813586No Hit
TATCAACGCAGAGTACTTTTTTTTT29350.1172613501795477No Hit
GGTATCAACGCAGAGTACTTTTTTT28270.11294645211501919No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA500.00150046913.299449
GGTATCA15900.012.32539751
ACGACCG855.3257507E-511.1764475
CTCCTAG3100.011.0476941
TACGAGA1658.0035534E-1110.9454974
GACCGTC1502.1591404E-910.7593327
CCGCGTC1158.827574E-710.7386799
CGTGGAC1355.6421413E-810.5618683
CTACGAC909.4619565E-510.5618683
CTAGATC1355.6454155E-810.5614454
ACCGCTC1002.4053332E-510.447898
ACCGAGC1303.8546023E-710.2287038
CTTATAC3650.010.1648871
CCGAGCG750.002649460710.1329079
AGACCGT2250.010.1266326
ATTATAC2201.8189894E-129.9377573
AAGACCG2700.09.8518315
TATACTG2450.09.6938575
GTTCTAA2201.6370905E-119.5132921
TATACAC4200.09.5056813
Produced by FastQC (version 0.11.5)