##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2502956
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.54338989578722	31.0	30.0	33.0	26.0	34.0
2	29.8001447088962	31.0	30.0	34.0	26.0	34.0
3	29.936683265706627	31.0	30.0	34.0	26.0	34.0
4	33.627588739074916	35.0	35.0	37.0	30.0	37.0
5	33.1784625858385	35.0	35.0	37.0	27.0	37.0
6	33.143550665692885	36.0	35.0	37.0	27.0	37.0
7	32.98863224123796	36.0	35.0	37.0	26.0	37.0
8	32.92845099953814	36.0	35.0	37.0	26.0	37.0
9	34.31083966318225	38.0	35.0	39.0	26.0	39.0
10	34.17652407793025	38.0	34.0	39.0	26.0	39.0
11	34.21134210909021	38.0	35.0	39.0	26.0	39.0
12	34.07409359173713	38.0	34.0	39.0	25.0	39.0
13	34.0622499956052	38.0	34.0	39.0	25.0	39.0
14	34.99199027070392	39.0	34.0	41.0	25.0	41.0
15	34.98536810075766	39.0	34.0	41.0	25.0	41.0
16	34.96498500173395	39.0	34.0	41.0	25.0	41.0
17	34.94359189694106	39.0	34.0	41.0	25.0	41.0
18	34.89582477678393	39.0	34.0	41.0	25.0	41.0
19	34.949878463704515	39.0	34.0	41.0	25.0	41.0
20	34.87341567330788	39.0	34.0	41.0	24.0	41.0
21	34.80030531899082	39.0	34.0	41.0	23.0	41.0
22	34.716913521452234	39.0	34.0	41.0	23.0	41.0
23	34.58831717377373	39.0	34.0	41.0	21.0	41.0
24	34.50922908752691	39.0	34.0	41.0	19.0	41.0
25	34.37206846624551	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79986.0
3	3.0
4	1266.0
5	10839.0
6	12231.0
7	9197.0
8	7233.0
9	6249.0
10	5470.0
11	5039.0
12	4820.0
13	4520.0
14	4424.0
15	4468.0
16	4522.0
17	4742.0
18	5165.0
19	6464.0
20	7769.0
21	10181.0
22	13131.0
23	15642.0
24	19070.0
25	22074.0
26	24957.0
27	28155.0
28	32478.0
29	39167.0
30	50203.0
31	63823.0
32	81670.0
33	105306.0
34	137280.0
35	181950.0
36	245950.0
37	368143.0
38	879369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.13383437236542	18.927181367491013	9.083412120601311	31.85557213954225
2	14.681600475597653	20.962094419558312	35.15471306726926	29.20159203757477
3	17.732875921149557	23.51100223390134	28.366550075322728	30.38957176962638
4	12.991033898013738	16.39510652068152	33.637333587044424	36.97652599426032
5	14.180152583862954	37.609327581154375	33.32201927547652	14.888500559506149
6	34.197202749146385	35.60206098080376	16.069809012475996	14.130927257573855
7	29.650516111401437	32.098229767458214	19.52249906909772	18.72875505204263
8	27.53790993349331	35.2662465150509	18.568456026714546	18.62738752474124
9	26.678894874700156	15.631517293951633	18.89250150621905	38.797086325129165
10	16.05749361954425	28.83418645793214	31.864004001668427	23.24431592085518
11	35.697910515503125	22.57836266048294	22.057969231064273	19.66575759294966
12	23.090697559205996	24.95445385376331	29.241824466750515	22.71302412028018
13	28.57455061793405	21.126061103415708	25.320453531191557	24.978934747458688
14	22.356963570667055	20.839564387829626	25.974912685287677	30.828559356215646
15	24.44091895412822	28.420642500629395	22.59989370247082	24.538544842771568
16	24.355220839409498	27.539823008849556	24.344593379811823	23.760362771929124
17	22.96384754666083	27.51950094208608	25.92598511519979	23.590666396053305
18	23.043269807023854	27.28878500938911	26.61908186503656	23.04886331855048
19	24.157077116206892	26.492889442558187	26.57691204355911	22.773121397675812
20	24.380492505661305	26.275132283587887	26.31025075950196	23.034124451248843
21	24.45203240858017	25.810993633515185	26.242471413947772	23.494502543956873
22	24.414452351539538	25.791344314482558	25.89122621412442	23.902977119853485
23	24.061853635383336	25.67315809681491	26.115797375966913	24.149190891834845
24	23.688009285247684	26.14448468673276	26.272656264046233	23.89484976397332
25	23.767627899828543	25.841712705855606	26.298329141645947	24.0923302526699
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	729.0
1	729.0
2	814.0
3	899.0
4	899.0
5	899.0
6	1268.0
7	1637.0
8	1637.0
9	1637.0
10	2291.0
11	2945.0
12	2945.0
13	2945.0
14	4200.0
15	5455.0
16	5455.0
17	5455.0
18	10685.0
19	15915.0
20	15915.0
21	15915.0
22	26795.5
23	37676.0
24	37676.0
25	37676.0
26	57307.5
27	76939.0
28	76939.0
29	76939.0
30	99504.5
31	122070.0
32	122070.0
33	122070.0
34	147858.0
35	173646.0
36	173646.0
37	173646.0
38	204041.5
39	234437.0
40	234437.0
41	234437.0
42	261953.0
43	289469.0
44	289469.0
45	289469.0
46	314235.5
47	339002.0
48	339002.0
49	339002.0
50	346234.5
51	353467.0
52	353467.0
53	353467.0
54	330461.0
55	307455.0
56	307455.0
57	307455.0
58	270128.0
59	232801.0
60	232801.0
61	232801.0
62	194512.0
63	156223.0
64	156223.0
65	156223.0
66	121931.0
67	87639.0
68	87639.0
69	87639.0
70	63952.0
71	40265.0
72	40265.0
73	40265.0
74	28128.0
75	15991.0
76	15991.0
77	15991.0
78	10774.5
79	5558.0
80	5558.0
81	5558.0
82	3784.5
83	2011.0
84	2011.0
85	2011.0
86	1271.5
87	532.0
88	532.0
89	532.0
90	342.5
91	153.0
92	153.0
93	153.0
94	96.0
95	39.0
96	39.0
97	39.0
98	21.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10195944315441421
2	0.0
3	0.042150161648866376
4	0.05685277727614868
5	0.06564238444463266
6	0.0027966931899721767
7	3.1962207885396306E-4
8	0.001997637992837269
9	0.0
10	0.0
11	0.001678015913983306
12	0.0
13	7.591024372781624E-4
14	0.05485513928331141
15	0.02181420688178298
16	2.3971655914047232E-4
17	0.0
18	0.0022373545519777417
19	0.0023172600716912326
20	0.0
21	0.0064723470967927525
22	0.0
23	5.992913978511808E-4
24	0.0024371183512614685
25	0.1741940329754099
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2502956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.433199451053866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.0564108943429	22.855368067734364
2	14.711034995848655	11.013622142620722
3	7.4516882373672555	8.368215961093215
4	4.460959585680283	6.679519596554427
5	2.8906733846626143	5.410357667796429
6	2.065147574156507	4.638304862355634
7	1.4466207928269526	3.7906151267553096
8	1.054560521442628	3.158045946589541
9	0.8159572554356174	2.748950161763038
>10	3.8741618599301835	24.247467701661705
>50	0.12677911881258036	3.1848587212823145
>100	0.04331684232500466	2.65328434012034
>500	0.00161336230131762	0.4137344549708767
>1k	0.0010755748675440318	0.8376552487020915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4690	0.18737844372813586	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2935	0.1172613501795477	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2827	0.11294645211501919	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.995275985674538E-5	0.0
10	0.0	0.0	0.0	3.995275985674538E-5	0.0
11	0.0	0.0	0.0	3.995275985674538E-5	0.0
12	0.0	0.0	0.0	3.995275985674538E-5	3.995275985674538E-5
13	0.0	0.0	0.0	3.995275985674538E-5	3.995275985674538E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTA	50	0.001500469	13.29944	9
GGTATCA	1590	0.0	12.3253975	1
ACGACCG	85	5.3257507E-5	11.176447	5
CTCCTAG	310	0.0	11.047694	1
TACGAGA	165	8.0035534E-11	10.945497	4
GACCGTC	150	2.1591404E-9	10.759332	7
CCGCGTC	115	8.827574E-7	10.738679	9
CGTGGAC	135	5.6421413E-8	10.561868	3
CTACGAC	90	9.4619565E-5	10.561868	3
CTAGATC	135	5.6454155E-8	10.561445	4
ACCGCTC	100	2.4053332E-5	10.44789	8
ACCGAGC	130	3.8546023E-7	10.228703	8
CTTATAC	365	0.0	10.164887	1
CCGAGCG	75	0.0026494607	10.132907	9
AGACCGT	225	0.0	10.126632	6
ATTATAC	220	1.8189894E-12	9.937757	3
AAGACCG	270	0.0	9.851831	5
TATACTG	245	0.0	9.693857	5
GTTCTAA	220	1.6370905E-11	9.513292	1
TATACAC	420	0.0	9.505681	3
>>END_MODULE