FastQCFastQC Report
Mon 15 Aug 2016
SRR2921175_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921175_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929662
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35510.18402186496909823No Hit
TATCAACGCAGAGTACTTTTTTTTT20690.10722085007633463No Hit
GGTATCAACGCAGAGTACTTTTTTT19670.10193495026590148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15200.012.6912061
TACACGA600.005875096711.0836962
TACGACC600.005892125411.0793884
CATAACG600.005892125411.0793884
TAGAGGT2150.010.6008764
TGTGCGA909.53876E-510.55316510
GTATCAA32350.010.3105851
CTATTAG1303.8265898E-710.2337581
TAGACTG2053.6379788E-1210.1914925
CTTACAC1409.7366865E-810.1752123
ACGCATA750.002655900810.1297255
GTATTAG1803.9108272E-1010.0307071
GGTTTAG951.6394503E-410.0029211
GGGCGTC2107.2759576E-129.95115911
AGGGCGT2500.09.87776310
CAACTCA3850.09.866366
TGGACTG2750.09.6692845
GTATTGC1002.747539E-49.5027751
GTCCTAA1401.0543663E-69.5027751
GTCTTAC1401.0543663E-69.5027751
Produced by FastQC (version 0.11.5)