##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921175_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1929662
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.881691715958546	31.0	27.0	31.0	10.0	34.0
2	26.657149283138704	31.0	26.0	31.0	10.0	34.0
3	26.38623188931533	31.0	26.0	31.0	10.0	34.0
4	30.286913459455594	35.0	30.0	37.0	10.0	37.0
5	29.893592245688623	35.0	30.0	37.0	10.0	37.0
6	30.323815258838078	35.0	31.0	37.0	8.0	37.0
7	30.254648223367617	35.0	30.0	37.0	2.0	37.0
8	29.9604060192925	35.0	30.0	37.0	2.0	37.0
9	31.47082546062471	37.0	31.0	39.0	2.0	39.0
10	30.856731386118398	37.0	30.0	39.0	2.0	39.0
11	31.277350644827955	37.0	30.0	39.0	2.0	39.0
12	30.98769059037282	37.0	30.0	39.0	2.0	39.0
13	30.96931379692402	37.0	30.0	39.0	2.0	39.0
14	32.050141941956674	38.0	31.0	40.0	2.0	41.0
15	31.690772269962306	38.0	30.0	40.0	2.0	41.0
16	31.62208303837667	38.0	30.0	40.0	2.0	41.0
17	31.944981556355465	38.0	31.0	40.0	2.0	41.0
18	32.002077047690214	38.0	31.0	40.0	2.0	41.0
19	31.704796487675043	38.0	30.0	40.0	2.0	41.0
20	31.556836896824418	38.0	30.0	40.0	2.0	41.0
21	31.505347568641554	38.0	30.0	40.0	2.0	41.0
22	30.74112668436234	38.0	28.0	40.0	2.0	41.0
23	30.834648762322107	38.0	30.0	40.0	2.0	41.0
24	30.454325679834085	37.0	29.0	40.0	2.0	41.0
25	29.958323789347563	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	169501.0
3	6.0
4	1396.0
5	7799.0
6	9275.0
7	8239.0
8	6951.0
9	6357.0
10	6007.0
11	5815.0
12	5535.0
13	5397.0
14	5282.0
15	5540.0
16	5960.0
17	6445.0
18	7609.0
19	9333.0
20	11427.0
21	14734.0
22	17534.0
23	20479.0
24	23720.0
25	26400.0
26	29240.0
27	32867.0
28	36618.0
29	42968.0
30	51103.0
31	60238.0
32	75344.0
33	94663.0
34	120587.0
35	154730.0
36	202707.0
37	264423.0
38	377433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.08325220245705	18.358647203550888	12.817071387913417	24.741029206078647
2	15.859154637464743	20.275427244068357	38.722726895636306	25.142691222830592
3	18.89451533653298	23.974903855124445	30.25744021395474	26.873140594387834
4	13.526313661792402	16.458179360585948	35.03566293884177	34.97984403877988
5	13.667650868134107	37.35606895733209	33.93074725198408	15.04553292254972
6	33.12562673445116	34.666894687883	17.445177739946985	14.762300837718856
7	28.591640098010355	32.68836106285681	20.577562510624006	18.142436328508822
8	26.079083841765982	34.541047655422844	20.603402840865805	18.776465661945366
9	26.914451941310574	16.07563847887613	19.332205953003477	37.677703626809816
10	16.064952389046837	28.529465722912235	32.89794040002114	22.507641488019782
11	34.36853935612403	23.52214674384844	22.444286542788152	19.665027357239367
12	22.955054182402602	25.65202091110603	29.321000611038812	22.071924295452554
13	28.23023342592976	22.24807205937228	25.228035988225052	24.293658526472907
14	22.417674117265953	22.04408163265306	26.04568837058633	29.492555879494653
15	24.479488957506064	28.733080216797113	23.255004026853985	23.532426798842838
16	23.709309456260243	28.005865938729034	25.234888961346286	23.049935643664437
17	22.71420395281178	27.906338972998867	25.929881241459114	23.449575832730243
18	22.394303704955387	27.704750126214588	26.909364966976874	22.99158120185315
19	23.961782224608733	27.092036546758415	27.154699683418993	21.791481545213863
20	24.352521472046043	26.439058573036057	26.670274063696475	22.538145891221433
21	25.004341058430384	26.071178844868975	26.06817249992614	22.856307596774503
22	23.958632949047836	25.38953281974341	27.30707339256979	23.344760838638962
23	24.440285356509047	25.85379936311015	26.324469673096377	23.38144560728443
24	23.815069465823726	26.128644714160288	26.607229669986804	23.44905615002918
25	23.479733247803793	25.95528235161805	27.17501551611385	23.38996888446431
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2019.0
1	2019.0
2	2059.0
3	2099.0
4	2099.0
5	2099.0
6	2534.5
7	2970.0
8	2970.0
9	2970.0
10	3761.5
11	4553.0
12	4553.0
13	4553.0
14	6628.0
15	8703.0
16	8703.0
17	8703.0
18	14194.5
19	19686.0
20	19686.0
21	19686.0
22	28640.5
23	37595.0
24	37595.0
25	37595.0
26	53766.0
27	69937.0
28	69937.0
29	69937.0
30	88210.5
31	106484.0
32	106484.0
33	106484.0
34	125923.0
35	145362.0
36	145362.0
37	145362.0
38	164755.0
39	184148.0
40	184148.0
41	184148.0
42	202973.5
43	221799.0
44	221799.0
45	221799.0
46	239647.0
47	257495.0
48	257495.0
49	257495.0
50	259264.0
51	261033.0
52	261033.0
53	261033.0
54	241867.0
55	222701.0
56	222701.0
57	222701.0
58	195319.0
59	167937.0
60	167937.0
61	167937.0
62	138319.5
63	108702.0
64	108702.0
65	108702.0
66	84634.5
67	60567.0
68	60567.0
69	60567.0
70	44401.5
71	28236.0
72	28236.0
73	28236.0
74	19927.0
75	11618.0
76	11618.0
77	11618.0
78	7830.5
79	4043.0
80	4043.0
81	4043.0
82	2746.0
83	1449.0
84	1449.0
85	1449.0
86	931.5
87	414.0
88	414.0
89	414.0
90	257.5
91	101.0
92	101.0
93	101.0
94	54.0
95	7.0
96	7.0
97	7.0
98	5.5
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03523933206955415
2	0.05296264319865344
3	0.01461395829943275
4	0.010779089809510681
5	0.00927623594183852
6	8.809833017388539E-4
7	0.003627578301277633
8	0.002021079339283253
9	0.00595959292352754
10	0.0033166430183109792
11	0.009898106507771827
12	0.008343430092938557
13	0.007358801696877484
14	0.014873071035238295
15	0.006011415470688649
16	0.028968803863059955
17	0.03658671829574298
18	0.02114359924173249
19	0.015443119054010494
20	0.01518400631820495
21	0.0211954217888936
22	0.005545012546238667
23	0.014821248488077187
24	0.011608250564088426
25	0.4704450831285479
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1929662.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.795144056379755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.79765711618496	35.03377021894804
2	11.86897063765199	11.582962641303407
3	5.585348825193648	8.176137015913664
4	3.1533790920808222	6.154783482498387
5	1.9778541499175923	4.825483908386872
6	1.3253298820945345	3.880179751141657
7	0.9522185940726321	3.2524550429656234
8	0.6359710111884991	2.482583768529945
9	0.479070579437708	2.103868614315265
>10	2.1247771957006183	16.801643694720326
>50	0.0695365003625653	2.2955870790000317
>100	0.027799304113408536	2.240285286964317
>500	0.0013181760006411624	0.45699777662414576
>1k	7.689360003740108E-4	0.7132617186884243
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3551	0.18402186496909823	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2069	0.10722085007633463	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1967	0.10193495026590148	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1520	0.0	12.691206	1
TACACGA	60	0.0058750967	11.083696	2
TACGACC	60	0.0058921254	11.079388	4
CATAACG	60	0.0058921254	11.079388	4
TAGAGGT	215	0.0	10.600876	4
TGTGCGA	90	9.53876E-5	10.553165	10
GTATCAA	3235	0.0	10.310585	1
CTATTAG	130	3.8265898E-7	10.233758	1
TAGACTG	205	3.6379788E-12	10.191492	5
CTTACAC	140	9.7366865E-8	10.175212	3
ACGCATA	75	0.0026559008	10.129725	5
GTATTAG	180	3.9108272E-10	10.030707	1
GGTTTAG	95	1.6394503E-4	10.002921	1
GGGCGTC	210	7.2759576E-12	9.951159	11
AGGGCGT	250	0.0	9.877763	10
CAACTCA	385	0.0	9.86636	6
TGGACTG	275	0.0	9.669284	5
GTATTGC	100	2.747539E-4	9.502775	1
GTCCTAA	140	1.0543663E-6	9.502775	1
GTCTTAC	140	1.0543663E-6	9.502775	1
>>END_MODULE