FastQCFastQC Report
Mon 15 Aug 2016
SRR2921175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929662
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65160.3376757173017865No Hit
GGTATCAACGCAGAGTACTTTTTTT47470.2460016313737846No Hit
TATCAACGCAGAGTACTTTTTTTTT45420.23537800920575727No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30770.15945797761473252No Hit
GGTATCAACGCAGAGTACATGGGGG30030.15562310912481045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG400.005280144514.2498255
GGTATCA19800.014.2243441
GGCGTCA2150.011.48882612
CCTATCC855.300971E-511.1812623
CGATCAA600.005874796311.08377210
CGCCAAA600.005874796311.08377214
TAGTACT951.3544948E-511.0044314
ACGCGTA951.3717303E-510.9918846
TCTAGAC2000.010.9296843
GTCCTAT1158.6421824E-710.7560131
CTAGACT1701.382432E-1010.6219244
AAGACCG1701.382432E-1010.6175175
CTATACC909.478103E-510.5598084
TAGGACA2250.010.5598084
CGCGTAT909.588361E-510.5477687
TACGCGT1002.39952E-510.4498725
TTATACA2550.010.4358452
TCTATAC1106.015647E-610.3680793
CCGTCTA750.002647196910.1337349
GGGCGTC2450.010.08203211
Produced by FastQC (version 0.11.5)