##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921175_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1929662
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.574867515658184	31.0	30.0	33.0	26.0	34.0
2	29.811184031193026	31.0	30.0	34.0	26.0	34.0
3	29.921657264329195	31.0	30.0	34.0	26.0	34.0
4	33.626829983696624	35.0	35.0	37.0	30.0	37.0
5	33.183882980542705	35.0	35.0	37.0	27.0	37.0
6	33.160191266657066	36.0	35.0	37.0	27.0	37.0
7	33.01585251717658	36.0	35.0	37.0	26.0	37.0
8	32.95022496167723	36.0	35.0	37.0	26.0	37.0
9	34.310970004073255	38.0	35.0	39.0	26.0	39.0
10	34.182592080892924	38.0	34.0	39.0	26.0	39.0
11	34.22045570675071	38.0	35.0	39.0	26.0	39.0
12	34.08841911174081	38.0	34.0	39.0	25.0	39.0
13	34.0642977889392	38.0	34.0	39.0	25.0	39.0
14	34.98686972122579	39.0	34.0	41.0	25.0	41.0
15	34.98044268892687	39.0	34.0	41.0	25.0	41.0
16	34.95700542374779	39.0	34.0	41.0	25.0	41.0
17	34.94927246326041	39.0	35.0	41.0	25.0	41.0
18	34.90071991882516	39.0	34.0	41.0	25.0	41.0
19	34.954313242422764	39.0	35.0	41.0	25.0	41.0
20	34.888944281433744	39.0	34.0	41.0	24.0	41.0
21	34.82240516732982	39.0	34.0	41.0	24.0	41.0
22	34.7322557007393	39.0	34.0	41.0	23.0	41.0
23	34.60325797989493	39.0	34.0	41.0	21.0	41.0
24	34.52106793832288	39.0	34.0	41.0	19.0	41.0
25	34.377985367385584	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61610.0
3	7.0
4	975.0
5	8211.0
6	9249.0
7	7181.0
8	5567.0
9	4753.0
10	4410.0
11	3978.0
12	3703.0
13	3375.0
14	3391.0
15	3457.0
16	3489.0
17	3563.0
18	4009.0
19	4831.0
20	6190.0
21	8023.0
22	10140.0
23	12147.0
24	14550.0
25	16488.0
26	18982.0
27	21396.0
28	24729.0
29	30254.0
30	38225.0
31	49138.0
32	62803.0
33	81033.0
34	106316.0
35	140926.0
36	191180.0
37	286624.0
38	674759.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.213382829047326	19.444636093933266	9.72117679587531	30.620804281144093
2	14.901158855799618	21.507445345350636	35.07951133410929	28.511884464740454
3	17.75198516801085	24.327147807656974	28.6660023517164	29.254864672615778
4	13.213617751174315	17.056877864491376	33.302637030215635	36.42686735411867
5	14.266422021393668	38.22098355282606	32.44922556079812	15.063368864982154
6	33.39486846735537	35.42848199112629	16.496322144200555	14.68032739731778
7	29.057915586152	33.14578500815698	19.3538055882042	18.44249381748682
8	27.568441324753724	34.74716201200703	18.740826810176124	18.94356985306312
9	27.175536441096938	15.74949395282697	19.090130810473543	37.98483879560254
10	16.93480557963868	28.901790419965394	31.253967669935466	22.90943633046046
11	34.776148487358114	23.130666511880047	22.059011017145036	20.034173983616803
12	23.269411948828346	25.630913600412924	28.72508242376126	22.374592026997476
13	28.490865050253365	21.72441027310012	25.296094260948514	24.488630415697994
14	22.692728507796257	21.68136728735954	25.851158160011202	29.774746044833005
15	24.758040438554296	28.54018767934449	22.90130662309874	23.800465259002472
16	24.17948380423077	27.769284268373706	24.6807851250377	23.370446802357822
17	22.910974805872133	27.676591060790113	25.97473332034311	23.437700812994645
18	22.620482708224284	27.242540929656446	27.097946131959596	23.03903023015968
19	24.13274674292549	26.64341104153311	27.105379491482296	22.118462724059103
20	24.307091163964543	26.105443719214055	26.83417502092343	22.75329009589797
21	24.862274480779067	25.545809770076517	26.43438491412531	23.15753083501911
22	24.66344985341924	25.336990640286107	26.23015640635907	23.769403099935584
23	24.379229230033317	25.522070381228634	26.39609613920564	23.70260424953241
24	23.832890215650608	25.97783893320066	26.566123005322595	23.623147845826143
25	23.831151468897076	25.767286830157232	26.756017974097983	23.645543726847706
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1224.0
1	1224.0
2	1306.5
3	1389.0
4	1389.0
5	1389.0
6	1868.5
7	2348.0
8	2348.0
9	2348.0
10	2966.0
11	3584.0
12	3584.0
13	3584.0
14	4973.0
15	6362.0
16	6362.0
17	6362.0
18	11598.0
19	16834.0
20	16834.0
21	16834.0
22	25649.5
23	34465.0
24	34465.0
25	34465.0
26	50483.0
27	66501.0
28	66501.0
29	66501.0
30	82724.0
31	98947.0
32	98947.0
33	98947.0
34	117891.5
35	136836.0
36	136836.0
37	136836.0
38	155430.0
39	174024.0
40	174024.0
41	174024.0
42	192875.0
43	211726.0
44	211726.0
45	211726.0
46	232949.5
47	254173.0
48	254173.0
49	254173.0
50	262428.0
51	270683.0
52	270683.0
53	270683.0
54	251729.0
55	232775.0
56	232775.0
57	232775.0
58	206434.0
59	180093.0
60	180093.0
61	180093.0
62	149761.5
63	119430.0
64	119430.0
65	119430.0
66	93316.5
67	67203.0
68	67203.0
69	67203.0
70	49227.5
71	31252.0
72	31252.0
73	31252.0
74	22171.0
75	13090.0
76	13090.0
77	13090.0
78	8747.5
79	4405.0
80	4405.0
81	4405.0
82	3063.0
83	1721.0
84	1721.0
85	1721.0
86	1084.5
87	448.0
88	448.0
89	448.0
90	289.5
91	131.0
92	131.0
93	131.0
94	72.5
95	14.0
96	14.0
97	14.0
98	9.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10120943460564596
2	0.0
3	0.04446374546423156
4	0.05653839895276997
5	0.07400259734606371
6	0.007306979149716375
7	0.0013473862261888351
8	0.00652964094229974
9	0.0
10	1.5546764148332713E-4
11	0.006425995847977521
12	0.0
13	0.0020729018864443617
14	0.0607360252728198
15	0.021454534524699143
16	0.00435309396153316
17	3.1093528296665426E-4
18	0.006840576225266393
19	0.004819496885983141
20	3.627578301277633E-4
21	0.014562135752271643
22	4.664029244499814E-4
23	0.004404916508694269
24	0.007877027168488576
25	0.18433280025206486
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1929662.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.37768450428503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.31745829319532	20.391968667995545
2	14.321366543491726	9.846708414047631
3	7.7961702243214015	8.040428409402665
4	4.750768735803445	6.5328171500908745
5	3.182144429826528	5.4697378627822175
6	2.2219598902090048	4.583150165206859
7	1.610391104137575	3.875306211458414
8	1.2134062723290144	3.3371278404517937
9	0.948487522758967	2.934612433229277
>10	4.412155117263122	25.259239987582134
>50	0.1587031681433855	3.694178518873328
>100	0.0627166097158071	3.7472045194257086
>500	0.001983469802231121	0.49372704898919806
>1k	0.0021360444024027454	1.4520180492051389
>5k	1.5257460017162469E-4	0.34177472125928127
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6516	0.3376757173017865	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4747	0.2460016313737846	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4542	0.23537800920575727	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3077	0.15945797761473252	No Hit
GGTATCAACGCAGAGTACATGGGGG	3003	0.15562310912481045	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.0364509432221809E-4	0.0	0.0	0.0	0.0
6	1.0364509432221809E-4	0.0	0.0	0.0	0.0
7	1.0364509432221809E-4	0.0	0.0	0.0	0.0
8	1.0364509432221809E-4	0.0	0.0	0.0	0.0
9	1.0364509432221809E-4	0.0	0.0	0.0	0.0
10	1.0364509432221809E-4	0.0	0.0	0.0	0.0
11	1.0364509432221809E-4	0.0	0.0	0.0	0.0
12	1.0364509432221809E-4	0.0	0.0	0.0	0.0
13	1.0364509432221809E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	40	0.0052801445	14.249825	5
GGTATCA	1980	0.0	14.224344	1
GGCGTCA	215	0.0	11.488826	12
CCTATCC	85	5.300971E-5	11.181262	3
CGATCAA	60	0.0058747963	11.083772	10
CGCCAAA	60	0.0058747963	11.083772	14
TAGTACT	95	1.3544948E-5	11.004431	4
ACGCGTA	95	1.3717303E-5	10.991884	6
TCTAGAC	200	0.0	10.929684	3
GTCCTAT	115	8.6421824E-7	10.756013	1
CTAGACT	170	1.382432E-10	10.621924	4
AAGACCG	170	1.382432E-10	10.617517	5
CTATACC	90	9.478103E-5	10.559808	4
TAGGACA	225	0.0	10.559808	4
CGCGTAT	90	9.588361E-5	10.547768	7
TACGCGT	100	2.39952E-5	10.449872	5
TTATACA	255	0.0	10.435845	2
TCTATAC	110	6.015647E-6	10.368079	3
CCGTCTA	75	0.0026471969	10.133734	9
GGGCGTC	245	0.0	10.082032	11
>>END_MODULE