FastQCFastQC Report
Mon 15 Aug 2016
SRR2921174_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921174_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814940
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30180.10721365286649094No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAC803.7859182E-410.6834623
GGTATCA17150.010.637221
GTATCAA34150.010.1831341
GTATCGA750.002657810710.1291469
AATACGG750.002657810710.1291465
GTCCTAG3700.09.7582721
CCATGCG2054.1836756E-119.7276859
GTATTAT2700.09.5014751
TACACGG1651.0732583E-79.2083145
TAGCACT3000.09.1798644
CGGATTT1151.1139948E-49.08481518
CATGGGT10650.09.0059864
CGATACT850.00746097238.93779854
TAATACA3200.08.90288454
GTCTAAG2252.6193447E-108.8680441
ACCGTGC2151.0495569E-98.83348
ATAGCAC1856.6193024E-88.7264323
GTATAAT3600.08.7096861
CTATTAG1651.0482345E-68.6377061
AATACAG4100.08.5696275
Produced by FastQC (version 0.11.5)