##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921174_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2814940
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.344709656333706	30.0	26.0	31.0	7.0	34.0
2	26.194754772748265	31.0	26.0	31.0	7.0	34.0
3	25.945283736065424	31.0	25.0	31.0	7.0	34.0
4	29.774281512216955	35.0	30.0	37.0	7.0	37.0
5	29.423159641058067	35.0	28.0	37.0	2.0	37.0
6	29.78555812912531	35.0	30.0	37.0	2.0	37.0
7	29.73341634279949	35.0	30.0	37.0	2.0	37.0
8	29.3248424477964	35.0	29.0	37.0	2.0	37.0
9	30.9099366238712	37.0	30.0	39.0	2.0	39.0
10	30.251850483491655	37.0	28.0	39.0	2.0	39.0
11	30.69026515662856	37.0	30.0	39.0	2.0	39.0
12	30.409005165296595	37.0	30.0	39.0	2.0	39.0
13	30.404654450894157	37.0	30.0	39.0	2.0	39.0
14	31.434904473985235	38.0	30.0	40.0	2.0	41.0
15	31.086561702913738	37.0	29.0	40.0	2.0	41.0
16	31.005010053500254	37.0	29.0	40.0	2.0	41.0
17	31.303265788968858	38.0	30.0	40.0	2.0	41.0
18	31.35593582811712	38.0	30.0	40.0	2.0	41.0
19	31.08504373094986	38.0	30.0	40.0	2.0	41.0
20	30.930219471818226	38.0	29.0	40.0	2.0	41.0
21	30.89192913525688	38.0	30.0	40.0	2.0	41.0
22	30.159101082083456	37.0	27.0	40.0	2.0	41.0
23	30.21542519556367	37.0	27.0	40.0	2.0	41.0
24	29.843016192174613	37.0	27.0	40.0	2.0	41.0
25	29.367284204991936	37.0	26.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	277900.0
3	11.0
4	2306.0
5	12632.0
6	14879.0
7	13082.0
8	10929.0
9	10142.0
10	9553.0
11	9167.0
12	8607.0
13	8484.0
14	8466.0
15	8397.0
16	9043.0
17	9838.0
18	11308.0
19	13985.0
20	17398.0
21	21781.0
22	26851.0
23	31201.0
24	36267.0
25	40616.0
26	45004.0
27	50374.0
28	55786.0
29	64541.0
30	75515.0
31	89898.0
32	111238.0
33	139075.0
34	174808.0
35	222535.0
36	288044.0
37	373119.0
38	512160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.90768031255586	18.41692191185308	12.426718067521191	25.248679708069872
2	15.650072526278114	20.038258900152588	39.02382935194101	25.287839221628296
3	18.589206310856397	24.09590995251402	30.137590313073474	27.177293423556105
4	13.145765421681396	15.982555402584714	36.341146689168184	34.530532486565704
5	13.538975769356073	37.10797649022493	34.66149895955425	14.691548780864752
6	32.91481404116061	35.11107227051246	17.655467785275906	14.318645903051024
7	28.406786164069427	32.56747707244981	21.09014883590393	17.93558792757683
8	26.150882238841817	34.45516760193315	20.8709488561518	18.523001303073237
9	26.838216877314835	15.724768849532369	19.546314471218327	37.89069980193447
10	15.63396918835673	28.468601290657215	33.48237647188388	22.41505304910217
11	35.03435451213182	23.0594648022133	22.489512805089703	19.41666788056518
12	22.873622635409394	25.247974476899	29.831012486210813	22.047390401480797
13	28.304329460031642	21.767027067163244	25.59292353571681	24.335719937088303
14	22.162413368948616	21.187256663831374	26.563942296418364	30.08638767080165
15	24.29322972769414	28.619222275591376	23.197760674384035	23.889787322330445
16	23.770827062752947	27.533424044274714	25.332516958551448	23.363231934420895
17	22.85985497336724	27.853052851844534	26.25181780833796	23.03527436645026
18	22.944719704140834	27.450794368215035	26.864612025958746	22.739873901685385
19	23.925061370499453	26.94613501758595	26.97381347702919	22.15499013488541
20	24.342437192750076	26.411555368586214	26.451740354537456	22.794267084126254
21	24.661821595590187	26.122165294078144	26.139256766144246	23.076756344187423
22	23.952024754999048	25.548025870537185	27.235330584751587	23.26461878971218
23	24.060808088416604	25.824713074534888	26.47818755342879	23.63629128361972
24	23.67370996686251	26.226259371990064	26.60545569464197	23.49457496650545
25	23.502314180314762	25.817815048310834	27.084154217775648	23.59571655359876
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1056.0
1	1056.0
2	1188.5
3	1321.0
4	1321.0
5	1321.0
6	1914.0
7	2507.0
8	2507.0
9	2507.0
10	3713.0
11	4919.0
12	4919.0
13	4919.0
14	7839.5
15	10760.0
16	10760.0
17	10760.0
18	18805.0
19	26850.0
20	26850.0
21	26850.0
22	41265.0
23	55680.0
24	55680.0
25	55680.0
26	78581.5
27	101483.0
28	101483.0
29	101483.0
30	128536.5
31	155590.0
32	155590.0
33	155590.0
34	187235.0
35	218880.0
36	218880.0
37	218880.0
38	250016.5
39	281153.0
40	281153.0
41	281153.0
42	308241.0
43	335329.0
44	335329.0
45	335329.0
46	357063.0
47	378797.0
48	378797.0
49	378797.0
50	376686.5
51	374576.0
52	374576.0
53	374576.0
54	347866.0
55	321156.0
56	321156.0
57	321156.0
58	279228.5
59	237301.0
60	237301.0
61	237301.0
62	197095.5
63	156890.0
64	156890.0
65	156890.0
66	121430.0
67	85970.0
68	85970.0
69	85970.0
70	63092.0
71	40214.0
72	40214.0
73	40214.0
74	28153.5
75	16093.0
76	16093.0
77	16093.0
78	10882.0
79	5671.0
80	5671.0
81	5671.0
82	3851.0
83	2031.0
84	2031.0
85	2031.0
86	1291.5
87	552.0
88	552.0
89	552.0
90	346.0
91	140.0
92	140.0
93	140.0
94	78.0
95	16.0
96	16.0
97	16.0
98	10.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03477871642024342
2	0.05247003488529063
3	0.015098012746275232
4	0.011190291800180465
5	0.009982450780478446
6	5.683957739774204E-4
7	0.003374849907990934
8	0.001989385208920972
9	0.007637818212821588
10	0.003374849907990934
11	0.011261341271927643
12	0.008348312930293363
13	0.007140471910591344
14	0.01484933959516011
15	0.00582605668326856
16	0.031013094417643005
17	0.03950350629143073
18	0.022309534128613754
19	0.01694529901170185
20	0.016199279558356483
21	0.02383709777117807
22	0.0055063340604062615
23	0.015844032199620595
24	0.010763994969697401
25	0.4648056441700356
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2814940.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.288930882842415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.57214685633	32.66709228086464
2	11.748980112309143	10.87695456725136
3	5.665093534914811	7.866933692475272
4	3.2854924800500167	6.08327737300535
5	2.1424316464000333	4.958543520071271
6	1.4792856528102758	4.1084730803349165
7	1.0651930702044288	3.4514653882502864
8	0.7575618630790062	2.8053382975633157
9	0.5799169855931635	2.415936353751744
>10	2.5986216037986574	19.585542351794416
>50	0.07888661907616493	2.4765569465139525
>100	0.024880241290859816	1.9174951955687989
>500	8.738250304708167E-4	0.28920624903794506
>1k	6.355091130516629E-4	0.49718470351671873
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3018	0.10721365286649094	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.5524735873588777E-5	0.0
4	0.0	0.0	0.0	3.5524735873588777E-5	0.0
5	0.0	0.0	0.0	3.5524735873588777E-5	0.0
6	0.0	0.0	0.0	3.5524735873588777E-5	0.0
7	0.0	0.0	0.0	3.5524735873588777E-5	0.0
8	0.0	0.0	0.0	3.5524735873588777E-5	0.0
9	0.0	0.0	0.0	7.104947174717755E-5	0.0
10	0.0	0.0	0.0	7.104947174717755E-5	0.0
11	0.0	0.0	0.0	7.104947174717755E-5	0.0
12	0.0	0.0	0.0	7.104947174717755E-5	0.0
13	0.0	0.0	0.0	7.104947174717755E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAC	80	3.7859182E-4	10.683462	3
GGTATCA	1715	0.0	10.63722	1
GTATCAA	3415	0.0	10.183134	1
GTATCGA	75	0.0026578107	10.129146	9
AATACGG	75	0.0026578107	10.129146	5
GTCCTAG	370	0.0	9.758272	1
CCATGCG	205	4.1836756E-11	9.727685	9
GTATTAT	270	0.0	9.501475	1
TACACGG	165	1.0732583E-7	9.208314	5
TAGCACT	300	0.0	9.179864	4
CGGATTT	115	1.1139948E-4	9.084815	18
CATGGGT	1065	0.0	9.005986	4
CGATACT	85	0.0074609723	8.9377985	4
TAATACA	320	0.0	8.9028845	4
GTCTAAG	225	2.6193447E-10	8.868044	1
ACCGTGC	215	1.0495569E-9	8.8334	8
ATAGCAC	185	6.6193024E-8	8.726432	3
GTATAAT	360	0.0	8.709686	1
CTATTAG	165	1.0482345E-6	8.637706	1
AATACAG	410	0.0	8.569627	5
>>END_MODULE