FastQCFastQC Report
Mon 15 Aug 2016
SRR2921174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814940
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56690.20138972766737478No Hit
TATCAACGCAGAGTACTTTTTTTTT37380.13279146269547487No Hit
GGTATCAACGCAGAGTACTTTTTTT35590.12643253497410248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19950.011.1109771
GACCGCT700.001501886810.8485977
CGCACTG803.7766382E-410.6862995
TCGCGCA1002.4188459E-510.44270118
TAGACTG2950.09.981935
CTAGACT2500.09.8845144
CTACTAG1502.5962618E-79.5134761
CAAGCGT1002.7440046E-49.5043394
TAAGGCG1002.759729E-49.4989345
GTTCGCG1106.8924564E-59.49336416
GACTTCG800.0045468099.4925227
GTATCAA37750.09.3244671
ATAAGAC1951.6698323E-99.2606383
GTATTAT1856.542905E-99.2563551
TAGGCTG3000.09.1823025
GTCTAGG2401.0913936E-119.1170811
CGTACTG1054.499374E-49.0466035
GTCTTAA2850.09.0127681
TCGGAAC950.0018168489.0041113
TCGTCCC950.00182303189.000759
Produced by FastQC (version 0.11.5)