##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921174_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2814940
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.818221347524283	31.0	30.0	33.0	26.0	34.0
2	30.053660113537056	31.0	30.0	34.0	26.0	34.0
3	30.184249753103085	31.0	30.0	34.0	26.0	34.0
4	33.87693130226577	35.0	35.0	37.0	30.0	37.0
5	33.49431604226022	35.0	35.0	37.0	28.0	37.0
6	33.47900985456173	37.0	35.0	37.0	28.0	37.0
7	33.34477964006338	37.0	35.0	37.0	28.0	37.0
8	33.2966002827769	37.0	35.0	37.0	27.0	37.0
9	34.70560082985783	39.0	35.0	39.0	27.0	39.0
10	34.575412619807175	38.0	35.0	39.0	27.0	39.0
11	34.63197119654416	39.0	35.0	39.0	27.0	39.0
12	34.50507826099313	38.0	35.0	39.0	27.0	39.0
13	34.49712036491009	39.0	35.0	39.0	27.0	39.0
14	35.466274947245765	39.0	36.0	41.0	27.0	41.0
15	35.469265774758966	39.0	36.0	41.0	27.0	41.0
16	35.44960176771086	39.0	36.0	41.0	27.0	41.0
17	35.42785956361414	39.0	36.0	41.0	27.0	41.0
18	35.386303793331294	39.0	36.0	41.0	27.0	41.0
19	35.426188124791295	39.0	36.0	41.0	26.0	41.0
20	35.36567600019894	39.0	35.0	41.0	26.0	41.0
21	35.29995346259601	39.0	35.0	41.0	26.0	41.0
22	35.22589326948354	39.0	35.0	41.0	25.0	41.0
23	35.109335190092864	39.0	35.0	41.0	25.0	41.0
24	35.03828749458248	39.0	35.0	41.0	25.0	41.0
25	34.907344028647145	39.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78233.0
3	12.0
4	1212.0
5	10312.0
6	12013.0
7	9013.0
8	7043.0
9	6099.0
10	5318.0
11	5020.0
12	4613.0
13	4379.0
14	4377.0
15	4483.0
16	4442.0
17	4771.0
18	5297.0
19	6339.0
20	8055.0
21	10333.0
22	13011.0
23	16191.0
24	19412.0
25	22534.0
26	25612.0
27	29397.0
28	33689.0
29	41728.0
30	53118.0
31	68342.0
32	87660.0
33	114585.0
34	151238.0
35	201319.0
36	275888.0
37	421587.0
38	1048265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.21800510359385	19.54716983815861	9.481618243214411	30.75320681503313
2	14.9175470880374	21.258890065152368	35.623068342486874	28.20049450432336
3	17.683912210749362	24.639894970773284	28.67622729274302	28.99996552573433
4	13.191474539064366	16.650979879206997	34.196710892981045	35.960834688747596
5	14.147807888069208	37.96552360373581	33.2402259462807	14.64644256191428
6	33.29327187813739	35.92034650612717	16.660579883346916	14.125801732388531
7	29.061214223850364	32.41649715410734	20.25823696044028	18.264051661602014
8	27.30031457991769	35.33867521177748	19.081341378716207	18.27966882958863
9	27.11222974557184	15.566015616673889	19.225063411653533	38.09669122610073
10	16.322443817630216	28.95415177588155	31.959118133956675	22.764286272531564
11	35.53569449299581	22.87007511488912	22.148483349959218	19.44574704215585
12	23.074630365123237	25.27645349456827	29.32630890889326	22.322607231415233
13	28.551828948784745	21.458348949509453	25.484995504317524	24.504826597388277
14	22.475852206582047	20.91847815847948	26.3362288527963	30.269440782142166
15	24.501270120907552	28.615154253332715	22.762897616777746	24.12067800898199
16	24.346511977372106	27.4418609608115	24.648082210127203	23.563544851689187
17	23.04763156585931	27.66492358629314	26.19491001584403	23.092534832003526
18	23.157803866176604	27.14746734667342	26.965718536602353	22.729010250547628
19	24.033267622756156	26.732718289909762	26.687813891161543	22.54620019617254
20	24.415618094879466	26.279849659317783	26.387240935863645	22.91729130993911
21	24.55697929479076	25.924785071273416	26.224679836313808	23.293555797622016
22	24.52426077333935	25.730860570492748	25.993708558101698	23.751170098066208
23	24.260652893372306	25.742935135542233	26.214707650246044	23.781704320839413
24	23.714592453068814	26.24455302668049	26.36796920350738	23.67288531674332
25	23.831546847037956	25.79459370190617	26.454293333029614	23.91956611802626
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	783.0
1	783.0
2	838.5
3	894.0
4	894.0
5	894.0
6	1443.5
7	1993.0
8	1993.0
9	1993.0
10	2818.5
11	3644.0
12	3644.0
13	3644.0
14	5654.5
15	7665.0
16	7665.0
17	7665.0
18	15029.0
19	22393.0
20	22393.0
21	22393.0
22	36051.0
23	49709.0
24	49709.0
25	49709.0
26	71687.5
27	93666.0
28	93666.0
29	93666.0
30	119265.5
31	144865.0
32	144865.0
33	144865.0
34	176215.5
35	207566.0
36	207566.0
37	207566.0
38	238077.0
39	268588.0
40	268588.0
41	268588.0
42	296789.0
43	324990.0
44	324990.0
45	324990.0
46	351365.0
47	377740.0
48	377740.0
49	377740.0
50	381506.5
51	385273.0
52	385273.0
53	385273.0
54	361766.0
55	338259.0
56	338259.0
57	338259.0
58	295603.5
59	252948.0
60	252948.0
61	252948.0
62	211601.0
63	170254.0
64	170254.0
65	170254.0
66	132046.5
67	93839.0
68	93839.0
69	93839.0
70	68486.5
71	43134.0
72	43134.0
73	43134.0
74	30193.0
75	17252.0
76	17252.0
77	17252.0
78	11748.5
79	6245.0
80	6245.0
81	6245.0
82	4244.5
83	2244.0
84	2244.0
85	2244.0
86	1472.5
87	701.0
88	701.0
89	701.0
90	461.5
91	222.0
92	222.0
93	222.0
94	138.5
95	55.0
96	55.0
97	55.0
98	36.5
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09961135938954295
2	0.0
3	0.04429934563436521
4	0.054423895358338005
5	0.0731454311637193
6	0.0034814241156117002
7	2.131484152415327E-4
8	0.0026643551905191584
9	0.0
10	0.0
11	0.0018472862654266165
12	0.0
13	6.039205098510093E-4
14	0.06191961462766524
15	0.022771355694970408
16	2.486731511151215E-4
17	0.0
18	0.0023446325676568596
19	0.0025222562470248035
20	0.0
21	0.010160074459846392
22	1.776236793679439E-4
23	8.525936609661308E-4
24	0.00262883045464557
25	0.18927579273448103
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2814940.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.952296777504564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.241313236025704	18.289914284515934
2	14.393158982138981	9.197889747262206
3	7.824107735678252	7.499946371686822
4	4.964129575239027	6.344613657201002
5	3.377525969649564	5.395985607798588
6	2.4635251798078306	4.722917259844505
7	1.7734254037098756	3.9665510368472647
8	1.3561070774852462	3.466458864150637
9	1.0386553081197913	2.9868680389116617
>10	5.299263858826865	28.688982721270417
>50	0.19813977191975607	4.267462566522876
>100	0.06696329117699422	3.595054003070193
>500	0.0021214422490965167	0.45390944663768334
>1k	0.0014515131177774703	0.9211975022772532
>5k	1.1165485521365156E-4	0.202248892003011
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5669	0.20138972766737478	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3738	0.13279146269547487	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3559	0.12643253497410248	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1995	0.0	11.110977	1
GACCGCT	70	0.0015018868	10.848597	7
CGCACTG	80	3.7766382E-4	10.686299	5
TCGCGCA	100	2.4188459E-5	10.442701	18
TAGACTG	295	0.0	9.98193	5
CTAGACT	250	0.0	9.884514	4
CTACTAG	150	2.5962618E-7	9.513476	1
CAAGCGT	100	2.7440046E-4	9.504339	4
TAAGGCG	100	2.759729E-4	9.498934	5
GTTCGCG	110	6.8924564E-5	9.493364	16
GACTTCG	80	0.004546809	9.492522	7
GTATCAA	3775	0.0	9.324467	1
ATAAGAC	195	1.6698323E-9	9.260638	3
GTATTAT	185	6.542905E-9	9.256355	1
TAGGCTG	300	0.0	9.182302	5
GTCTAGG	240	1.0913936E-11	9.117081	1
CGTACTG	105	4.499374E-4	9.046603	5
GTCTTAA	285	0.0	9.012768	1
TCGGAAC	95	0.001816848	9.004111	3
TCGTCCC	95	0.0018230318	9.00075	9
>>END_MODULE