FastQCFastQC Report
Mon 15 Aug 2016
SRR2921173_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921173_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434516
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14630.10198561744867259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8100.012.6697611
TAGGACG604.106151E-412.6613674
ATATGAC1052.7339047E-711.7569853
GTTAGGC855.344695E-511.1717953
TTAGGCT951.3662026E-510.9953984
CGCTCGC700.001493016810.85562815
ACTAGAC803.784898E-410.6830293
TATGACT1701.4006218E-1010.6132054
CTGCCCG1002.4091154E-510.4456289
GTATAAG1303.826135E-710.2332681
GCCTCGA750.002650423210.13191916
CTAGGGG856.617691E-410.0546153
AGGCGTG951.6511574E-49.9954677
GTATTAG1252.5604768E-69.8824131
GTTCTAG2054.1836756E-119.7340851
GTATCAA14750.09.6633771
GTATACA1002.747754E-49.5023211
GCGTTCA800.00452028879.49867413
AAAACGA800.00452028879.49867413
ACCCGAC800.00452158939.49834311
Produced by FastQC (version 0.11.5)