##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921173_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1434516
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.700954886526187	30.0	26.0	31.0	2.0	33.0
2	25.506120531245383	30.0	25.0	31.0	2.0	34.0
3	25.348682761293706	30.0	25.0	31.0	2.0	34.0
4	29.131794974751067	35.0	28.0	37.0	2.0	37.0
5	28.907292076212464	35.0	28.0	37.0	2.0	37.0
6	29.103501111176175	35.0	29.0	37.0	2.0	37.0
7	29.053377585192496	35.0	29.0	37.0	2.0	37.0
8	28.56924844337742	35.0	27.0	37.0	2.0	37.0
9	30.143840849457238	37.0	29.0	39.0	2.0	39.0
10	29.511076209676293	35.0	27.0	39.0	2.0	39.0
11	29.917657244673464	35.0	28.0	39.0	2.0	39.0
12	29.66481865660613	35.0	27.0	39.0	2.0	39.0
13	29.64619704485694	35.0	27.0	39.0	2.0	39.0
14	30.64495760242479	37.0	28.0	40.0	2.0	41.0
15	30.296580170594122	37.0	27.0	40.0	2.0	41.0
16	30.214162128550676	37.0	27.0	40.0	2.0	41.0
17	30.4869537878978	37.0	28.0	40.0	2.0	41.0
18	30.542182171547754	37.0	29.0	40.0	2.0	41.0
19	30.282887747505082	37.0	27.0	40.0	2.0	41.0
20	30.13253668833251	37.0	27.0	40.0	2.0	41.0
21	30.084812577900838	37.0	27.0	40.0	2.0	41.0
22	29.380159579955887	37.0	25.0	40.0	2.0	41.0
23	29.435134219485874	37.0	26.0	40.0	2.0	41.0
24	29.063355166481237	37.0	25.0	40.0	2.0	41.0
25	28.5943997836204	37.0	22.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	166987.0
3	9.0
4	1384.0
5	7230.0
6	8302.0
7	7173.0
8	6089.0
9	5509.0
10	5276.0
11	4876.0
12	4715.0
13	4479.0
14	4534.0
15	4556.0
16	4774.0
17	5351.0
18	6103.0
19	7342.0
20	9301.0
21	11623.0
22	14089.0
23	16493.0
24	18497.0
25	20711.0
26	23149.0
27	25426.0
28	28113.0
29	32668.0
30	38167.0
31	45462.0
32	55194.0
33	69165.0
34	87036.0
35	109968.0
36	142843.0
37	185021.0
38	246901.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.85390770732728	18.57547811230627	12.05111486898764	25.519499311378812
2	16.09068020616112	20.170243334286493	38.705146356262595	25.03393010328979
3	18.13745107417204	24.437396553334903	30.39125324022494	27.033899132268107
4	12.875127755724083	16.294031280457453	36.157554005987265	34.6732869578312
5	13.303322815476449	37.25705632932837	35.432930884713045	14.006689970482144
6	32.50749908156728	35.70726987422177	17.54955876888887	14.235672275322079
7	28.11131900796948	32.26601843744598	21.9753330842289	17.647329470355643
8	26.08428326046221	34.42874710002677	21.456664919246897	18.03030472026412
9	26.64637950927388	15.19382601148211	19.71890783983603	38.44088663940798
10	15.22513397055471	28.739585780542743	34.14734634778178	21.887933901120775
11	34.853591198739494	22.612421043825034	23.05408759429424	19.479900163141238
12	22.430069430355964	25.415804800238707	30.443318295550643	21.710807473854686
13	28.508535571095834	21.961936937093952	25.60043892641499	23.929088565395226
14	21.965075305761893	21.147316504518624	26.887454803295856	30.000153386423627
15	24.207448595876517	28.792165184764833	23.354489416613568	23.645896802745085
16	23.602577593126046	27.62977048933406	25.56596662182865	23.20168529571124
17	22.62119956817554	27.931343258600922	26.39896618769858	23.048490985524957
18	22.56412008501001	27.531404617538612	27.03851109759975	22.865964199851625
19	23.77766064404033	27.11855189233736	27.083969550806064	22.019817912816244
20	24.17684089576444	26.429150621818483	26.712153506230734	22.68185497618634
21	24.57930018868214	26.10040176884276	26.424006281037375	22.89629176143772
22	23.637271182163328	25.68071955706695	27.48930243063834	23.19270683013138
23	23.727640099730593	25.977225950034306	26.594197442032986	23.700936508202116
24	23.562134154333535	26.20563489473963	26.817689988761444	23.41454096216539
25	23.408776978014124	26.032680416126148	27.1550190383597	23.40352356750002
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	486.0
1	486.0
2	533.5
3	581.0
4	581.0
5	581.0
6	989.0
7	1397.0
8	1397.0
9	1397.0
10	2040.5
11	2684.0
12	2684.0
13	2684.0
14	4583.5
15	6483.0
16	6483.0
17	6483.0
18	10999.5
19	15516.0
20	15516.0
21	15516.0
22	22969.0
23	30422.0
24	30422.0
25	30422.0
26	42315.5
27	54209.0
28	54209.0
29	54209.0
30	68270.5
31	82332.0
32	82332.0
33	82332.0
34	97938.0
35	113544.0
36	113544.0
37	113544.0
38	130091.5
39	146639.0
40	146639.0
41	146639.0
42	160068.5
43	173498.0
44	173498.0
45	173498.0
46	181917.0
47	190336.0
48	190336.0
49	190336.0
50	188702.0
51	187068.0
52	187068.0
53	187068.0
54	172532.0
55	157996.0
56	157996.0
57	157996.0
58	138494.0
59	118992.0
60	118992.0
61	118992.0
62	97979.0
63	76966.0
64	76966.0
65	76966.0
66	59692.5
67	42419.0
68	42419.0
69	42419.0
70	31361.0
71	20303.0
72	20303.0
73	20303.0
74	14231.5
75	8160.0
76	8160.0
77	8160.0
78	5605.0
79	3050.0
80	3050.0
81	3050.0
82	2044.5
83	1039.0
84	1039.0
85	1039.0
86	672.0
87	305.0
88	305.0
89	305.0
90	189.5
91	74.0
92	74.0
93	74.0
94	41.5
95	9.0
96	9.0
97	9.0
98	8.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03422757222645129
2	0.04782100722473642
3	0.01408140445976204
4	0.009619969383401789
5	0.009062289998856757
6	4.879694614769023E-4
7	0.003067236614997672
8	0.001394198461362578
9	0.007737801460562309
10	0.004112885461019605
11	0.012268946459990687
12	0.008992580075788629
13	0.007458961768289793
14	0.01603328230566965
15	0.006831572460676632
16	0.032415114226679934
17	0.041825953840877346
18	0.023143694458618795
19	0.017985160151577258
20	0.017776030382372868
21	0.024956152458390148
22	0.005437373999314055
23	0.017566900613168485
24	0.011711267075445656
25	0.4790465913241818
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1434516.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.957851516686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.0903436024714	58.959122638145864
2	10.11793427931235	14.35893756494066
3	3.200204471887064	6.812389012175904
4	1.3602095378385188	3.8607018567010285
5	0.6755895169741765	2.3969190315841615
6	0.39464669936970165	1.6801969137255304
7	0.25573422824781983	1.270244597702016
8	0.1799489720128757	1.0215033949335934
9	0.11957454527027915	0.7636277545617344
>10	0.5789095238372296	6.816551383684071
>50	0.02041204755215145	0.9852149504598284
>100	0.0059931838946662265	0.7395105820982317
>500	2.996347990349297E-4	0.15931906889978342
>1k	1.9975653268995315E-4	0.1757612503876614
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1463	0.10198561744867259	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	6.97099230681289E-5
8	0.0	0.0	0.0	0.0	6.97099230681289E-5
9	0.0	0.0	0.0	0.0	6.97099230681289E-5
10	0.0	0.0	0.0	0.0	6.97099230681289E-5
11	6.97099230681289E-5	0.0	0.0	0.0	6.97099230681289E-5
12	6.97099230681289E-5	0.0	0.0	0.0	6.97099230681289E-5
13	6.97099230681289E-5	0.0	0.0	0.0	6.97099230681289E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	810	0.0	12.669761	1
TAGGACG	60	4.106151E-4	12.661367	4
ATATGAC	105	2.7339047E-7	11.756985	3
GTTAGGC	85	5.344695E-5	11.171795	3
TTAGGCT	95	1.3662026E-5	10.995398	4
CGCTCGC	70	0.0014930168	10.855628	15
ACTAGAC	80	3.784898E-4	10.683029	3
TATGACT	170	1.4006218E-10	10.613205	4
CTGCCCG	100	2.4091154E-5	10.445628	9
GTATAAG	130	3.826135E-7	10.233268	1
GCCTCGA	75	0.0026504232	10.131919	16
CTAGGGG	85	6.617691E-4	10.054615	3
AGGCGTG	95	1.6511574E-4	9.995467	7
GTATTAG	125	2.5604768E-6	9.882413	1
GTTCTAG	205	4.1836756E-11	9.734085	1
GTATCAA	1475	0.0	9.663377	1
GTATACA	100	2.747754E-4	9.502321	1
GCGTTCA	80	0.0045202887	9.498674	13
AAAACGA	80	0.0045202887	9.498674	13
ACCCGAC	80	0.0045215893	9.498343	11
>>END_MODULE