FastQCFastQC Report
Mon 15 Aug 2016
SRR2921173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434516
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24580.17134699090146083No Hit
GGTATCAACGCAGAGTACTTTTTTT20320.14165056367443793No Hit
TATCAACGCAGAGTACTTTTTTTTT19190.13377334236773936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.013.658371
ATTATAC1351.8189894E-1213.3771023
AGGATCG500.001500071313.2992665
AAAGGCG1153.783498E-1013.2166635
CGTGCGC853.938985E-612.295156510
GTTATAA701.0737018E-412.2349561
CCTATTG855.2947395E-511.1820973
TAATACC951.3528372E-511.0051544
CTTATAC1752.0008883E-1110.8755171
GGCCTAT909.351256E-510.5734191
GTAACAC1553.6288839E-910.4246013
CTAATAC1201.51311E-610.2968473
TAGGACT1856.002665E-1110.2750824
AAGACCG856.595614E-410.05826955
GACAGCG951.6578997E-49.9914317
GCGCCAC1151.0249307E-59.91388113
GACAGTC1356.5142194E-79.843417
ATAATAC1451.6039485E-79.8325333
GTGCTAA1106.708409E-59.5160771
GTATAGA1401.0336607E-69.5160771
Produced by FastQC (version 0.11.5)