##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921173_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1434516
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.676128394524703	31.0	30.0	33.0	26.0	34.0
2	29.927929698936783	31.0	30.0	34.0	26.0	34.0
3	30.06916827696589	31.0	30.0	34.0	26.0	34.0
4	33.75309860608038	35.0	35.0	37.0	30.0	37.0
5	33.34991383853509	35.0	35.0	37.0	28.0	37.0
6	33.319431083375854	37.0	35.0	37.0	28.0	37.0
7	33.17451879240106	37.0	35.0	37.0	27.0	37.0
8	33.117634100979004	37.0	35.0	37.0	26.0	37.0
9	34.515550889638035	39.0	35.0	39.0	27.0	39.0
10	34.37650747708635	38.0	35.0	39.0	27.0	39.0
11	34.42407264889342	39.0	35.0	39.0	27.0	39.0
12	34.30290983160871	38.0	35.0	39.0	26.0	39.0
13	34.29017173736647	38.0	35.0	39.0	26.0	39.0
14	35.25342136302418	39.0	35.0	41.0	25.0	41.0
15	35.25811144664821	39.0	35.0	41.0	25.0	41.0
16	35.23512181111957	39.0	35.0	41.0	25.0	41.0
17	35.202231972316795	39.0	35.0	41.0	25.0	41.0
18	35.160996461524306	39.0	35.0	41.0	25.0	41.0
19	35.20573629014943	39.0	35.0	41.0	25.0	41.0
20	35.14491368517326	39.0	35.0	41.0	25.0	41.0
21	35.07231498289318	39.0	35.0	41.0	25.0	41.0
22	34.99743049223571	39.0	35.0	41.0	25.0	41.0
23	34.8867283460066	39.0	35.0	41.0	24.0	41.0
24	34.81094599154	39.0	35.0	41.0	23.0	41.0
25	34.67922630350585	39.0	35.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43866.0
3	10.0
4	705.0
5	5826.0
6	6746.0
7	5052.0
8	3982.0
9	3496.0
10	2984.0
11	2695.0
12	2603.0
13	2413.0
14	2360.0
15	2376.0
16	2411.0
17	2520.0
18	2811.0
19	3406.0
20	4298.0
21	5555.0
22	7052.0
23	8465.0
24	9988.0
25	11872.0
26	13259.0
27	15149.0
28	17491.0
29	21186.0
30	27189.0
31	34630.0
32	44216.0
33	57712.0
34	75997.0
35	101084.0
36	139881.0
37	212348.0
38	530882.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.1156319456802	19.567967787745246	9.032480922822481	31.28391934375207
2	14.884671903276086	21.522729617515594	35.06200000557679	28.530598473631525
3	16.8613749529269	25.15167998661032	28.930220232366764	29.056724828096016
4	12.739240456886497	17.060355554563564	34.55643563803965	35.643968350510285
5	13.827450219853738	38.269967693394904	33.70218625933323	14.20039582741813
6	32.769788159904515	36.70298536492867	16.7879807394012	13.739245735765609
7	28.63568142732959	32.70508208023501	20.903150969215293	17.756085523220108
8	27.219751668059764	35.41814952201226	19.133870728243767	18.22822808168421
9	26.981365143365426	15.02653159671973	19.30992752956398	38.68217573035086
10	15.75416377370486	29.479838496050238	32.56791837804528	22.198079352199628
11	35.29396181825153	22.573102676626313	22.539291926201937	19.593643578920215
12	22.565450646768667	25.503933033859504	29.978682705525767	21.951933613846066
13	28.617338015100724	21.692490977698867	25.467950179191227	24.222220828009185
14	22.171982630835497	20.995204296949932	26.551872068081543	30.280941004133023
15	24.24212632053087	28.889530371774196	22.8708172003874	23.997526107307536
16	24.06584566394285	27.504172167979778	24.904113332394566	23.525868835682807
17	22.819264476659722	27.761419182497793	26.325743316909676	23.093573023932812
18	22.830137906899246	27.419366082794333	26.97069112396005	22.779804886346366
19	23.85880473132702	26.91316953875348	26.77618218690373	22.45184354301577
20	24.20614339610015	26.336339225216033	26.522813269423274	22.934704109260544
21	24.387432963441196	25.94924882799318	26.410940031736914	23.252378176828707
22	24.09328303065285	25.861754068968207	26.221457271999753	23.823505628379188
23	23.911902343605675	25.885483363878258	26.210196033597793	23.99241825891827
24	23.572687909527637	26.30541672737616	26.482700060721108	23.639195302375093
25	23.61598384042701	26.18380257047288	26.484699183709676	23.715514405390437
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	380.0
1	380.0
2	419.0
3	458.0
4	458.0
5	458.0
6	714.5
7	971.0
8	971.0
9	971.0
10	1485.5
11	2000.0
12	2000.0
13	2000.0
14	3383.5
15	4767.0
16	4767.0
17	4767.0
18	8897.0
19	13027.0
20	13027.0
21	13027.0
22	20065.5
23	27104.0
24	27104.0
25	27104.0
26	38873.5
27	50643.0
28	50643.0
29	50643.0
30	63952.5
31	77262.0
32	77262.0
33	77262.0
34	92477.0
35	107692.0
36	107692.0
37	107692.0
38	124316.5
39	140941.0
40	140941.0
41	140941.0
42	155330.5
43	169720.0
44	169720.0
45	169720.0
46	179696.5
47	189673.0
48	189673.0
49	189673.0
50	191429.0
51	193185.0
52	193185.0
53	193185.0
54	179923.5
55	166662.0
56	166662.0
57	166662.0
58	146203.0
59	125744.0
60	125744.0
61	125744.0
62	104261.5
63	82779.0
64	82779.0
65	82779.0
66	64328.5
67	45878.0
68	45878.0
69	45878.0
70	33825.0
71	21772.0
72	21772.0
73	21772.0
74	15276.0
75	8780.0
76	8780.0
77	8780.0
78	6095.0
79	3410.0
80	3410.0
81	3410.0
82	2281.0
83	1152.0
84	1152.0
85	1152.0
86	752.5
87	353.0
88	353.0
89	353.0
90	236.5
91	120.0
92	120.0
93	120.0
94	77.5
95	35.0
96	35.0
97	35.0
98	21.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10602879298662407
2	0.0
3	0.04015291568724225
4	0.05660445753132067
5	0.07410164822142103
6	0.005925343460790957
7	3.485496153406445E-4
8	0.004531144999428379
9	0.0
10	0.0
11	0.004252305307155863
12	0.0
13	0.0010456488460219336
14	0.06559703760710929
15	0.023631663920095698
16	6.97099230681289E-4
17	0.0
18	0.0048796946147690225
19	0.005367664076245925
20	0.0
21	0.015615022767260874
22	0.0
23	0.0011850686921581913
24	0.006204183153063473
25	0.19483923497542027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1434516.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.06837078494226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.93818964295298	42.93470211354371
2	14.440939800917299	16.771236936853917
3	5.08780827957906	8.86322212983888
4	2.3285651197157407	5.408639310741484
5	1.2485280997275046	3.6249996315198048
6	0.7401201969505533	2.578654441316953
7	0.4606353577486254	1.8723841325281192
8	0.3257651455145384	1.5133321006839107
9	0.24793912550958655	1.2957678964966504
>10	1.1198956695639766	11.07244969371271
>50	0.04521747832880778	1.7694898060975313
>100	0.015069987148330966	1.4183933712913244
>500	7.233252780296643E-4	0.2680448082921368
>1k	6.027710650247203E-4	0.6086836270828231
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2458	0.17134699090146083	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2032	0.14165056367443793	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1919	0.13377334236773936	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.394198461362578E-4
13	0.0	0.0	0.0	0.0	2.788396922725156E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	850	0.0	13.65837	1
ATTATAC	135	1.8189894E-12	13.377102	3
AGGATCG	50	0.0015000713	13.299266	5
AAAGGCG	115	3.783498E-10	13.216663	5
CGTGCGC	85	3.938985E-6	12.2951565	10
GTTATAA	70	1.0737018E-4	12.234956	1
CCTATTG	85	5.2947395E-5	11.182097	3
TAATACC	95	1.3528372E-5	11.005154	4
CTTATAC	175	2.0008883E-11	10.875517	1
GGCCTAT	90	9.351256E-5	10.573419	1
GTAACAC	155	3.6288839E-9	10.424601	3
CTAATAC	120	1.51311E-6	10.296847	3
TAGGACT	185	6.002665E-11	10.275082	4
AAGACCG	85	6.595614E-4	10.0582695	5
GACAGCG	95	1.6578997E-4	9.991431	7
GCGCCAC	115	1.0249307E-5	9.913881	13
GACAGTC	135	6.5142194E-7	9.84341	7
ATAATAC	145	1.6039485E-7	9.832533	3
GTGCTAA	110	6.708409E-5	9.516077	1
GTATAGA	140	1.0336607E-6	9.516077	1
>>END_MODULE