FastQCFastQC Report
Mon 15 Aug 2016
SRR2921172_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921172_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691650
Sequences flagged as poor quality0
Sequence length25
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18670.11036561936570803No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA350.002175230516.280199
TTAACGC400.005293329714.2443243
GGTATCA8550.012.6728611
TAGAGCG550.003078489812.0857355
GACAGTA1052.734305E-711.7572197
GTATCAA19500.010.6744481
GTCTATA856.5640116E-410.0637431
ACCGTCT951.649655E-49.9966078
CTAGAAC1752.590241E-99.7675363
TAGAGTG1752.590241E-99.7672475
GATATAA1304.2112715E-69.5046461
CGTCCAA1002.7621025E-49.4976210
ACCGTAT1106.8610556E-59.4967788
TGGACTG3600.09.49593455
CTTCTAG2850.09.3378971
TATACTC1356.8825375E-69.1442345
ACAGTAC2304.1836756E-119.0838748
CAGGACG950.00183074678.9961494
GTGCTAG1804.1824023E-88.976611
GCGCTAG850.0074470148.93946513
Produced by FastQC (version 0.11.5)