##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921172_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1691650
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.141292229480094	30.0	25.0	31.0	2.0	34.0
2	24.743517276032275	30.0	23.0	31.0	2.0	34.0
3	24.519703839446695	30.0	22.0	31.0	2.0	34.0
4	28.18747081252032	35.0	27.0	37.0	2.0	37.0
5	27.777345195519167	35.0	26.0	37.0	2.0	37.0
6	28.241365530694885	35.0	27.0	37.0	2.0	37.0
7	28.013607424703693	35.0	26.0	37.0	2.0	37.0
8	27.701981497354655	35.0	26.0	37.0	2.0	37.0
9	29.201519226790413	35.0	27.0	39.0	2.0	39.0
10	28.53928708657228	35.0	25.0	39.0	2.0	39.0
11	28.995509709455266	35.0	27.0	39.0	2.0	39.0
12	28.685898974374133	35.0	26.0	39.0	2.0	39.0
13	28.665230396358584	35.0	26.0	39.0	2.0	39.0
14	29.60960068572104	36.0	27.0	40.0	2.0	41.0
15	29.267838500871928	36.0	25.0	40.0	2.0	41.0
16	29.187194750687198	36.0	25.0	40.0	2.0	41.0
17	29.455458280377147	36.0	27.0	40.0	2.0	41.0
18	29.49270534685071	36.0	27.0	40.0	2.0	41.0
19	29.201241982679633	37.0	25.0	40.0	2.0	41.0
20	29.065476901250257	37.0	25.0	40.0	2.0	41.0
21	29.007576626370703	37.0	25.0	40.0	2.0	41.0
22	28.30443058552301	36.0	18.0	40.0	2.0	41.0
23	28.33468920876068	36.0	18.0	40.0	2.0	41.0
24	27.954293736884107	36.0	17.0	40.0	2.0	41.0
25	27.49633789495463	36.0	2.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	232080.0
3	9.0
4	1866.0
5	9214.0
6	10229.0
7	8967.0
8	7549.0
9	7002.0
10	6509.0
11	6173.0
12	5973.0
13	5850.0
14	5783.0
15	5808.0
16	6152.0
17	6688.0
18	7800.0
19	9422.0
20	11762.0
21	14633.0
22	17856.0
23	20548.0
24	23218.0
25	25865.0
26	28461.0
27	31148.0
28	34575.0
29	40264.0
30	46793.0
31	54980.0
32	66941.0
33	82762.0
34	103799.0
35	129338.0
36	163934.0
37	203276.0
38	248423.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.886279018057785	18.092200192073875	12.036787351509389	25.984733438358955
2	15.94967419649231	20.57491883745785	38.3731885922343	25.10221837381554
3	19.864748934741183	23.721497268786955	29.308792324342413	27.104961472129446
4	13.673491588272297	15.769269880008135	34.67963543661998	35.87760309509958
5	14.076522516429439	37.001530024664845	33.41750045522372	15.504447003681992
6	33.26243583165252	34.41267784474748	17.485298325179528	14.839587998420473
7	28.81705415999891	31.890950514867882	20.220927063683533	19.07106826144967
8	27.194840940018654	33.90300220024994	20.358105160360505	18.5440516993709
9	27.331157395282734	15.937041650433839	19.183863612313342	37.54793734197008
10	16.276789002594587	28.31315223052272	32.28785309047829	23.122205676404395
11	35.51261692575281	22.93323274949777	21.903352019382094	19.650798305367324
12	23.496336724565317	24.818994052091643	29.070675528918088	22.613993694424952
13	28.500596209678992	21.57983445648556	25.25948570486054	24.660083628974906
14	22.72511474270025	21.15983689184004	25.76403000367153	30.351018361788185
15	24.63766659414875	28.248467664891553	23.003057576072873	24.110808164886823
16	24.222580698571587	27.418000506177016	24.684171560893805	23.675247234357595
17	23.256071136259337	27.575708831474817	25.79949046454305	23.3687295677228
18	23.168652864293087	27.15338609494133	26.433565606605747	23.24439543415984
19	24.37145404537403	26.69011519696529	26.582627985460256	22.355802772200427
20	24.923996883014915	25.822317660511136	26.174988677870942	23.078696778603007
21	25.000798222388305	25.892678704254468	25.7829379077586	23.323585165598626
22	24.460109272819068	24.982589891141004	26.947951755376444	23.60934908066349
23	24.42530009855968	25.61245056495923	26.141551094059757	23.82069824242134
24	24.192006470215077	25.665304713194594	26.22482696726996	23.917861849320374
25	23.929504699706488	25.578961187546888	26.68113339467733	23.810400718069292
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1002.0
1	1002.0
2	1076.0
3	1150.0
4	1150.0
5	1150.0
6	1476.0
7	1802.0
8	1802.0
9	1802.0
10	2326.0
11	2850.0
12	2850.0
13	2850.0
14	4087.0
15	5324.0
16	5324.0
17	5324.0
18	9277.0
19	13230.0
20	13230.0
21	13230.0
22	20637.5
23	28045.0
24	28045.0
25	28045.0
26	40785.5
27	53526.0
28	53526.0
29	53526.0
30	69667.5
31	85809.0
32	85809.0
33	85809.0
34	103390.0
35	120971.0
36	120971.0
37	120971.0
38	141160.0
39	161349.0
40	161349.0
41	161349.0
42	179320.0
43	197291.0
44	197291.0
45	197291.0
46	212347.5
47	227404.0
48	227404.0
49	227404.0
50	227630.5
51	227857.0
52	227857.0
53	227857.0
54	213738.0
55	199619.0
56	199619.0
57	199619.0
58	176616.5
59	153614.0
60	153614.0
61	153614.0
62	128377.5
63	103141.0
64	103141.0
65	103141.0
66	81019.0
67	58897.0
68	58897.0
69	58897.0
70	43776.0
71	28655.0
72	28655.0
73	28655.0
74	20430.5
75	12206.0
76	12206.0
77	12206.0
78	8628.5
79	5051.0
80	5051.0
81	5051.0
82	3472.5
83	1894.0
84	1894.0
85	1894.0
86	1295.5
87	697.0
88	697.0
89	697.0
90	456.5
91	216.0
92	216.0
93	216.0
94	127.5
95	39.0
96	39.0
97	39.0
98	25.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037478201755682325
2	0.05361629178612597
3	0.01637454556202524
4	0.011941004344870393
5	0.01028581562379925
6	3.546832973723879E-4
7	0.003014808027665297
8	0.0014187331894895516
9	0.00703455206455236
10	0.003014808027665297
11	0.011349865515916413
12	0.007743918659297136
13	0.007743918659297136
14	0.015192267904117282
15	0.00673898265007537
16	0.03203972452930571
17	0.0412614902609878
18	0.023349983743682204
19	0.016906570508083824
20	0.016078976147548253
21	0.02317264209499601
22	0.0061478438211213906
23	0.017083912156770017
24	0.012236573759347382
25	0.48751219223834724
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1691650.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.50209768362361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.70820259363482	45.46092858951638
2	10.406765902190356	12.17635270761087
3	4.610895136321656	8.092411130221036
4	2.4193977076832085	5.661593641216724
5	1.3800262728389363	4.036721590979523
6	0.9020404601012175	3.1662755466853323
7	0.5935531182739734	2.4306871753978374
8	0.3969468387572207	1.8577778188944416
9	0.29745374712409206	1.5661501353552159
>10	1.226083655091098	11.781914333555864
>50	0.04326001953932941	1.733669038446068
>100	0.014024265869411974	1.3897697563349212
>500	0.0011425467939916022	0.46758586457804374
>1k	2.0773578072574584E-4	0.17816267120764448
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1867	0.11036561936570803	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1822776579079597E-4
2	0.0	0.0	0.0	0.0	1.1822776579079597E-4
3	0.0	0.0	0.0	0.0	1.1822776579079597E-4
4	0.0	0.0	0.0	0.0	1.1822776579079597E-4
5	0.0	0.0	0.0	0.0	1.1822776579079597E-4
6	0.0	0.0	0.0	0.0	1.7734164868619395E-4
7	0.0	0.0	0.0	0.0	1.7734164868619395E-4
8	0.0	0.0	0.0	0.0	1.7734164868619395E-4
9	0.0	0.0	0.0	0.0	1.7734164868619395E-4
10	0.0	0.0	0.0	0.0	1.7734164868619395E-4
11	0.0	0.0	0.0	0.0	1.7734164868619395E-4
12	0.0	0.0	0.0	0.0	3.546832973723879E-4
13	0.0	0.0	0.0	0.0	6.502527118493779E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTA	35	0.0021752305	16.28019	9
TTAACGC	40	0.0052933297	14.244324	3
GGTATCA	855	0.0	12.672861	1
TAGAGCG	55	0.0030784898	12.085735	5
GACAGTA	105	2.734305E-7	11.757219	7
GTATCAA	1950	0.0	10.674448	1
GTCTATA	85	6.5640116E-4	10.063743	1
ACCGTCT	95	1.649655E-4	9.996607	8
CTAGAAC	175	2.590241E-9	9.767536	3
TAGAGTG	175	2.590241E-9	9.767247	5
GATATAA	130	4.2112715E-6	9.504646	1
CGTCCAA	100	2.7621025E-4	9.49762	10
ACCGTAT	110	6.8610556E-5	9.496778	8
TGGACTG	360	0.0	9.4959345	5
CTTCTAG	285	0.0	9.337897	1
TATACTC	135	6.8825375E-6	9.144234	5
ACAGTAC	230	4.1836756E-11	9.083874	8
CAGGACG	95	0.0018307467	8.996149	4
GTGCTAG	180	4.1824023E-8	8.97661	1
GCGCTAG	85	0.007447014	8.939465	13
>>END_MODULE