FastQCFastQC Report
Mon 15 Aug 2016
SRR2921172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691650
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36480.21564744480241185No Hit
GGTATCAACGCAGAGTACTTTTTTT23390.1382673720923359No Hit
TATCAACGCAGAGTACTTTTTTTTT22530.13318357816333165No Hit
CTTATACACATCTCCGAGCCCACGA20480.12106523216977506No Hit
GGTATCAACGCAGAGTACATGGGGG17730.10480891437354063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGG400.005268133714.2545713
CTTATAC3750.012.9409921
GGTATCA10700.012.8058191
TATACAC4150.012.59443
TCGCCCG550.00306670612.0919259
CGACCAA550.00306670612.09192514
TAAGATA658.0044876E-411.6957124
TTATACA4600.011.1557522
CCGTAGG600.005862009211.0868882
ACCGTCT1201.2774399E-711.081978
ATAGGAC1053.4528985E-610.8606263
GTATCAA23750.010.7774821
TAGCACA2150.010.6077394
GTATACA909.359941E-510.5727061
CGTACAC1002.3902387E-510.4533523
ATCGAGC856.601039E-410.0575878
GAATCGA856.6334155E-410.0522366
TAGTACT951.6391932E-410.0029124
GATCTCG1252.5387344E-69.88931519
TGGACTG3000.09.8148795
Produced by FastQC (version 0.11.5)