##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921172_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1691650
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.22420477049035	31.0	30.0	33.0	25.0	34.0
2	29.480120592321107	31.0	30.0	34.0	25.0	34.0
3	29.623566931693908	31.0	30.0	34.0	25.0	34.0
4	33.29226317500665	35.0	35.0	37.0	28.0	37.0
5	32.82167883427423	35.0	33.0	37.0	26.0	37.0
6	32.77784470782964	35.0	33.0	37.0	26.0	37.0
7	32.60953329589454	35.0	33.0	37.0	25.0	37.0
8	32.550898235450596	36.0	33.0	37.0	25.0	37.0
9	33.890845624094815	38.0	34.0	39.0	24.0	39.0
10	33.741964354328616	38.0	34.0	39.0	23.0	39.0
11	33.79313451364053	38.0	34.0	39.0	23.0	39.0
12	33.64725090887595	38.0	34.0	39.0	22.0	39.0
13	33.63531522478054	38.0	34.0	39.0	22.0	39.0
14	34.553561907013865	39.0	34.0	41.0	19.0	41.0
15	34.54553365057784	39.0	34.0	41.0	19.0	41.0
16	34.515866757307954	39.0	34.0	41.0	19.0	41.0
17	34.49315106552774	39.0	34.0	41.0	19.0	41.0
18	34.44088079685514	39.0	34.0	41.0	18.0	41.0
19	34.47332604262111	39.0	34.0	41.0	18.0	41.0
20	34.39971152425147	39.0	34.0	41.0	18.0	41.0
21	34.337531995389114	39.0	34.0	41.0	18.0	41.0
22	34.25369313983389	39.0	34.0	41.0	17.0	41.0
23	34.11968019389354	39.0	34.0	41.0	15.0	41.0
24	34.04441935388526	39.0	34.0	41.0	9.0	41.0
25	33.905916708539	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66141.0
3	1.0
4	1044.0
5	8753.0
6	9731.0
7	7223.0
8	5556.0
9	4832.0
10	4166.0
11	3787.0
12	3576.0
13	3319.0
14	3244.0
15	3227.0
16	3225.0
17	3500.0
18	3800.0
19	4553.0
20	5909.0
21	7418.0
22	9069.0
23	11282.0
24	13365.0
25	15107.0
26	17334.0
27	19122.0
28	21812.0
29	26873.0
30	34035.0
31	42917.0
32	54608.0
33	70234.0
34	91464.0
35	121215.0
36	162974.0
37	244511.0
38	582723.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.16548947390846	19.073126393576885	9.375784071919218	31.385600060595443
2	14.99488664912955	20.93269884432359	35.57721750953212	28.495196997014748
3	18.047576975799917	24.134085084087037	28.119156515784283	29.699181424328764
4	13.462566718401028	16.280236211530454	33.40084036794488	36.856356702123634
5	14.568597039786956	37.72414250003253	32.61510723703591	15.092153223144603
6	33.66619984168544	35.196613321714935	16.451235509754554	14.685951326845077
7	29.500264543226955	32.30867908689728	19.52937907714317	18.6616772927326
8	27.469090917689677	35.22275702984321	18.776949752124352	18.53120230034276
9	27.305863408613163	15.837965907720536	18.873474560841405	37.9826961228249
10	16.69136646120615	28.984947805304532	31.04453825177475	23.279147481714567
11	35.92974374829671	22.70594515538441	21.660708722442166	19.703602373876716
12	23.522905756454204	24.859116755307987	28.72493052636806	22.89304696186975
13	28.73721609382944	21.268636422752692	25.2773146998664	24.716832783551475
14	22.64815410676938	20.867146318150414	25.742781568512402	30.741918006567804
15	24.767271351197937	28.19144533005369	22.55575790544216	24.485525413306213
16	24.661367888696102	27.32602854719095	24.09970530597044	23.912898258142512
17	23.224895707284027	27.512232263144547	25.825991807915784	23.43688022165564
18	23.31543486884148	26.923602149234533	26.499324004376973	23.261638977547015
19	24.397382563864888	26.485845473371327	26.335867257751485	22.7809047050123
20	24.81798469629041	25.691764561224296	26.078258895635887	23.411991846849403
21	24.79282217054025	25.625671724379295	26.077330624956403	23.504175480124054
22	24.844555266676835	25.22573330070147	25.770425361108252	24.159286071513446
23	24.398491351281052	25.37361519999054	26.002080894784758	24.22581255394365
24	24.01608201567884	25.729037780677107	26.01238251361598	24.242497690028074
25	24.06342250581077	25.594741439294122	26.11586006780464	24.22597598709047
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	658.0
1	658.0
2	688.0
3	718.0
4	718.0
5	718.0
6	969.5
7	1221.0
8	1221.0
9	1221.0
10	1573.5
11	1926.0
12	1926.0
13	1926.0
14	2756.5
15	3587.0
16	3587.0
17	3587.0
18	7063.0
19	10539.0
20	10539.0
21	10539.0
22	17827.0
23	25115.0
24	25115.0
25	25115.0
26	37955.5
27	50796.0
28	50796.0
29	50796.0
30	66536.0
31	82276.0
32	82276.0
33	82276.0
34	98613.0
35	114950.0
36	114950.0
37	114950.0
38	135169.0
39	155388.0
40	155388.0
41	155388.0
42	174168.0
43	192948.0
44	192948.0
45	192948.0
46	210246.0
47	227544.0
48	227544.0
49	227544.0
50	231260.0
51	234976.0
52	234976.0
53	234976.0
54	221347.0
55	207718.0
56	207718.0
57	207718.0
58	184165.0
59	160612.0
60	160612.0
61	160612.0
62	135333.5
63	110055.0
64	110055.0
65	110055.0
66	85928.0
67	61801.0
68	61801.0
69	61801.0
70	45598.5
71	29396.0
72	29396.0
73	29396.0
74	20635.5
75	11875.0
76	11875.0
77	11875.0
78	8376.0
79	4877.0
80	4877.0
81	4877.0
82	3312.5
83	1748.0
84	1748.0
85	1748.0
86	1211.0
87	674.0
88	674.0
89	674.0
90	441.0
91	208.0
92	208.0
93	208.0
94	124.0
95	40.0
96	40.0
97	40.0
98	22.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10368575059852805
2	0.0
3	0.039251618242544264
4	0.05686755534537286
5	0.06478881565335619
6	0.004788224514527237
7	0.003251263559246889
8	0.004492655100050247
9	3.546832973723879E-4
10	1.7734164868619395E-4
11	0.004197085685573257
12	5.911388289539798E-5
13	0.004137971802677859
14	0.05781337747169923
15	0.024236691987113174
16	0.0038424023882008694
17	0.0010049360092217658
18	0.004197085685573257
19	0.004197085685573257
20	0.0020098720184435316
21	0.006384299352702983
22	0.0030739219105606953
23	0.004137971802677859
24	0.004197085685573257
25	0.17586380161380902
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1691650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.09949954733871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.97154818062629	26.291986154922064
2	14.647210112914596	12.039860108110167
3	7.115092773350974	8.772802566528338
4	4.138149647406192	6.803035182415624
5	2.6036079240660577	5.35034913483002
6	1.7219078648518233	4.246173090722189
7	1.2098094683024607	3.480579458640415
8	0.8577775435843765	2.8203382211410712
9	0.6675786656654037	2.4693434160595418
>10	2.905688532181623	19.98047835167294
>50	0.11677318462548242	3.250068582708275
>100	0.04167296857906144	2.9539242474359453
>500	0.00202563062905899	0.5746794593570237
>1k	0.001157503216605137	0.9663820254564354
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3648	0.21564744480241185	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2339	0.1382673720923359	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2253	0.13318357816333165	No Hit
CTTATACACATCTCCGAGCCCACGA	2048	0.12106523216977506	No Hit
GGTATCAACGCAGAGTACATGGGGG	1773	0.10480891437354063	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7734164868619395E-4
2	0.0	0.0	0.0	0.0	1.7734164868619395E-4
3	0.0	0.0	0.0	0.0	1.7734164868619395E-4
4	0.0	0.0	0.0	0.0	1.7734164868619395E-4
5	0.0	0.0	0.0	0.0	1.7734164868619395E-4
6	0.0	0.0	0.0	0.0	1.7734164868619395E-4
7	0.0	0.0	0.0	0.0	1.7734164868619395E-4
8	0.0	0.0	0.0	0.0	1.7734164868619395E-4
9	0.0	0.0	0.0	0.0	1.7734164868619395E-4
10	0.0	0.0	0.0	0.0	2.3645553158159195E-4
11	0.0	0.0	0.0	0.0	2.3645553158159195E-4
12	0.0	0.0	0.0	0.0	5.320249460585818E-4
13	0.0	0.0	0.0	0.0	5.911388289539798E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTACGG	40	0.0052681337	14.254571	3
CTTATAC	375	0.0	12.940992	1
GGTATCA	1070	0.0	12.805819	1
TATACAC	415	0.0	12.5944	3
TCGCCCG	55	0.003066706	12.091925	9
CGACCAA	55	0.003066706	12.091925	14
TAAGATA	65	8.0044876E-4	11.695712	4
TTATACA	460	0.0	11.155752	2
CCGTAGG	60	0.0058620092	11.086888	2
ACCGTCT	120	1.2774399E-7	11.08197	8
ATAGGAC	105	3.4528985E-6	10.860626	3
GTATCAA	2375	0.0	10.777482	1
TAGCACA	215	0.0	10.607739	4
GTATACA	90	9.359941E-5	10.572706	1
CGTACAC	100	2.3902387E-5	10.453352	3
ATCGAGC	85	6.601039E-4	10.057587	8
GAATCGA	85	6.6334155E-4	10.052236	6
TAGTACT	95	1.6391932E-4	10.002912	4
GATCTCG	125	2.5387344E-6	9.889315	19
TGGACTG	300	0.0	9.814879	5
>>END_MODULE