FastQCFastQC Report
Mon 15 Aug 2016
SRR2921171_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921171_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345879
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13630.10127210544187107No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGAT250.00604462918.992946
GGTGCGC555.9312333E-717.2663088
GGTATCA9250.012.3275331
ACCGAGC802.8766115E-511.8705868
GATCTAC658.0046954E-411.6953511
GTCTTAA1657.2759576E-1211.5181481
ACCGTGC752.0805903E-411.3957638
TTAATGT855.3395575E-511.1727324
GCCCTAA600.005863591611.0862181
GACCGTG1158.853567E-710.7351397
GTATTAG803.7609084E-410.6902821
CTTACTC909.5406904E-510.5524183
GTATCAA19400.010.2861811
CCCTAGA1951.4551915E-1110.2334321
GTCTAGC750.002641466210.1359711
GAACCGT750.002655350810.1295676
GTCTTAC856.575961E-410.0614411
ATATACT1054.1182026E-59.9490534
TATATAC1356.4632513E-79.8489233
TGCTACT1451.6127706E-79.8286822
Produced by FastQC (version 0.11.5)