##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921171_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1345879
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.602453861008307	30.0	26.0	31.0	2.0	34.0
2	25.302492274565545	30.0	25.0	31.0	2.0	34.0
3	24.94156161140786	30.0	23.0	31.0	2.0	34.0
4	28.542267915615	35.0	27.0	37.0	2.0	37.0
5	28.333485402476747	35.0	27.0	37.0	2.0	37.0
6	28.696372407920773	35.0	28.0	37.0	2.0	37.0
7	28.557343565060457	35.0	28.0	37.0	2.0	37.0
8	28.21050406462988	35.0	27.0	37.0	2.0	37.0
9	29.651225704539563	35.0	28.0	39.0	2.0	39.0
10	28.947881644635217	35.0	27.0	39.0	2.0	39.0
11	29.472527619496255	35.0	27.0	39.0	2.0	39.0
12	29.161854817557895	35.0	27.0	39.0	2.0	39.0
13	29.172325298188024	35.0	27.0	39.0	2.0	39.0
14	30.186134117554403	37.0	27.0	40.0	2.0	41.0
15	29.8240287574143	37.0	27.0	40.0	2.0	41.0
16	29.77769472590032	37.0	27.0	40.0	2.0	41.0
17	30.073655209717963	37.0	27.0	40.0	2.0	41.0
18	30.122741345990242	37.0	27.0	40.0	2.0	41.0
19	29.807292483202428	37.0	27.0	40.0	2.0	41.0
20	29.639573839847415	37.0	27.0	40.0	2.0	41.0
21	29.585369115648582	37.0	27.0	40.0	2.0	41.0
22	28.882576368306513	36.0	22.0	40.0	2.0	41.0
23	28.946139288895957	37.0	24.0	40.0	2.0	41.0
24	28.565926803226738	36.0	21.0	40.0	2.0	41.0
25	28.097219735206508	36.0	18.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	168556.0
3	17.0
4	1216.0
5	6629.0
6	8107.0
7	6939.0
8	5683.0
9	5094.0
10	4813.0
11	4696.0
12	4437.0
13	4374.0
14	4423.0
15	4406.0
16	4734.0
17	5148.0
18	6089.0
19	7323.0
20	9195.0
21	11603.0
22	13846.0
23	16031.0
24	18267.0
25	20111.0
26	21986.0
27	24720.0
28	27372.0
29	31925.0
30	36987.0
31	43926.0
32	53775.0
33	66316.0
34	83165.0
35	104555.0
36	132372.0
37	166073.0
38	210970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56406208423084	17.810863402502456	12.145550981939916	25.47952353132679
2	16.282254389362492	19.997145250968867	38.64713146044946	25.073468899219183
3	19.366155840025208	24.208976696971455	29.377659471694972	27.04720799130837
4	14.460860990585772	15.903647762662468	34.068895000404986	35.566596246346776
5	14.270820871289159	37.41095134099645	33.38777621153361	14.93045157618077
6	34.28654646180275	33.82305410110494	17.01718369321234	14.873215743879978
7	29.28042596951756	32.329635970230875	20.10307439902989	18.286863661221673
8	27.277009816888135	34.03663426073156	20.202638641625626	18.48371728075468
9	27.443733332144433	16.07574497744429	18.8837840388355	37.59673765157578
10	16.717713460303873	28.523194711938558	31.916580474118454	22.842511353639107
11	35.588017391472356	22.991015198589302	21.75531705403142	19.665650355906923
12	23.74275315472767	24.948096165878265	28.63122493048527	22.67792574890879
13	28.53598383660646	21.834551111381924	25.091825641627725	24.537639410383896
14	22.868483202763297	21.267309633051383	25.568317779373057	30.29588938481226
15	24.716763400934624	28.275806723669415	22.97547227153263	24.031957603863326
16	24.39785050132671	27.451967029128234	24.66074043242681	23.489442037118245
17	23.32660382155	27.524421735232856	25.53767956721013	23.611294876007012
18	23.348447497170273	27.482336048729987	26.233243678917784	22.935972775181952
19	24.631964436729096	26.535568472821474	26.41503264573911	22.41743444471032
20	25.11722959164716	26.125664177163454	25.67019655928362	23.08690967190577
21	25.272102043561596	25.79330426452369	25.63871983281099	23.29587385910372
22	24.633894788778026	24.995913175032026	26.716094957006604	23.654097079183348
23	24.55391081857764	25.433344307054124	26.11562239203828	23.897122482329955
24	24.43125831357891	25.769010693398926	26.008516077254047	23.79121491576812
25	24.00857265067643	25.441826682263258	26.610227312596436	23.93937335446388
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	840.0
1	840.0
2	934.5
3	1029.0
4	1029.0
5	1029.0
6	1326.5
7	1624.0
8	1624.0
9	1624.0
10	2015.5
11	2407.0
12	2407.0
13	2407.0
14	3109.5
15	3812.0
16	3812.0
17	3812.0
18	6616.0
19	9420.0
20	9420.0
21	9420.0
22	14681.0
23	19942.0
24	19942.0
25	19942.0
26	29768.0
27	39594.0
28	39594.0
29	39594.0
30	52032.0
31	64470.0
32	64470.0
33	64470.0
34	79017.0
35	93564.0
36	93564.0
37	93564.0
38	110167.0
39	126770.0
40	126770.0
41	126770.0
42	142960.0
43	159150.0
44	159150.0
45	159150.0
46	171859.5
47	184569.0
48	184569.0
49	184569.0
50	186775.0
51	188981.0
52	188981.0
53	188981.0
54	176194.0
55	163407.0
56	163407.0
57	163407.0
58	143791.5
59	124176.0
60	124176.0
61	124176.0
62	103246.0
63	82316.0
64	82316.0
65	82316.0
66	63927.5
67	45539.0
68	45539.0
69	45539.0
70	33517.5
71	21496.0
72	21496.0
73	21496.0
74	14991.5
75	8487.0
76	8487.0
77	8487.0
78	5728.0
79	2969.0
80	2969.0
81	2969.0
82	1968.5
83	968.0
84	968.0
85	968.0
86	616.5
87	265.0
88	265.0
89	265.0
90	166.0
91	67.0
92	67.0
93	67.0
94	39.0
95	11.0
96	11.0
97	11.0
98	8.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03573872539804841
2	0.05587426507137715
3	0.01612329191554367
4	0.011145132660514059
5	0.009361911434831808
6	7.430088440342705E-4
7	0.004086548642188488
8	0.002600530954119947
9	0.0074300884403427055
10	0.004086548642188488
11	0.012036743273355183
12	0.008990407012814674
13	0.0090647078972181
14	0.01790651314122592
15	0.0071328849027289965
16	0.03336109709713875
17	0.041682796150322575
18	0.02541090246597205
19	0.016420495453157376
20	0.017014902528384796
21	0.02496509715955149
22	0.006761380480711862
23	0.017832212256822494
24	0.014042867152247715
25	0.46586654520948767
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1345879.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.57543150168352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.92256168621164	45.05772242984714
2	11.085575963395573	12.986847910011734
3	4.786303956821724	8.41079458707143
4	2.456034446003091	5.754531098305172
5	1.409520856763294	4.128164619776629
6	0.8647003844149679	3.039011888206702
7	0.5832102750423049	2.3913255461772978
8	0.38877447426680317	1.821810606961452
9	0.2889419023599903	1.5232406948688362
>10	1.1578923137215944	11.100128771307732
>50	0.04089023759287128	1.616312449759006
>100	0.0145452119168051	1.6566941463979004
>500	7.86218617084757E-4	0.3309682409395164
>1k	2.6207287236158566E-4	0.18244701036948757
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1363	0.10127210544187107	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	7.430088440342706E-5	0.0	0.0	0.0
11	0.0	7.430088440342706E-5	0.0	0.0	0.0
12	0.0	7.430088440342706E-5	0.0	0.0	0.0
13	0.0	7.430088440342706E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCGAT	25	0.006044629	18.99294	6
GGTGCGC	55	5.9312333E-7	17.266308	8
GGTATCA	925	0.0	12.327533	1
ACCGAGC	80	2.8766115E-5	11.870586	8
GATCTAC	65	8.0046954E-4	11.695351	1
GTCTTAA	165	7.2759576E-12	11.518148	1
ACCGTGC	75	2.0805903E-4	11.395763	8
TTAATGT	85	5.3395575E-5	11.172732	4
GCCCTAA	60	0.0058635916	11.086218	1
GACCGTG	115	8.853567E-7	10.735139	7
GTATTAG	80	3.7609084E-4	10.690282	1
CTTACTC	90	9.5406904E-5	10.552418	3
GTATCAA	1940	0.0	10.286181	1
CCCTAGA	195	1.4551915E-11	10.233432	1
GTCTAGC	75	0.0026414662	10.135971	1
GAACCGT	75	0.0026553508	10.129567	6
GTCTTAC	85	6.575961E-4	10.061441	1
ATATACT	105	4.1182026E-5	9.949053	4
TATATAC	135	6.4632513E-7	9.848923	3
TGCTACT	145	1.6127706E-7	9.828682	2
>>END_MODULE