FastQCFastQC Report
Mon 15 Aug 2016
SRR2921171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345879
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27600.20507044095345867No Hit
TATCAACGCAGAGTACTTTTTTTTT18900.14042867152247715No Hit
CTTATACACATCTCCGAGCCCACGA17390.12920923797755965No Hit
GGTATCAACGCAGAGTACATGGGGG17080.1269059105610534No Hit
GGTATCAACGCAGAGTACTTTTTTT16450.1222249548436375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC2400.014.667361
TTGCGCG400.00530147814.2407218
GGTATCA9850.013.812151
CGTGTCA500.001499165313.30023510
GGGCGGT500.00150800913.2898576
TTATACA3150.012.3712772
TATACAC3150.012.3712773
AGTAGTC550.003069381212.0902225
ATGGTCG550.003077938712.0857315
TCGATAA550.003083082812.083034518
ATTTGCG550.003083082812.083034516
GTGTTAG1052.6672933E-711.7791921
GCCCTAT907.3373867E-611.6281791
GTCGTAG600.00581107611.0996241
TTACACT600.00585308711.0888884
TCTATAC600.00585308711.0888883
GTGTTAA700.001473013910.8731011
GAATAGG1053.404035E-610.87311
CCCTATA700.001484954610.8625852
ATAGAAC700.001484954610.8625853
Produced by FastQC (version 0.11.5)