##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921171_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1345879
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.355781611868526	31.0	30.0	33.0	25.0	34.0
2	29.598853240150117	31.0	30.0	34.0	26.0	34.0
3	29.742415922976733	31.0	30.0	34.0	26.0	34.0
4	33.42773087328059	35.0	35.0	37.0	28.0	37.0
5	32.960599727018554	35.0	33.0	37.0	26.0	37.0
6	32.92416703136018	36.0	35.0	37.0	26.0	37.0
7	32.76194368141564	36.0	33.0	37.0	25.0	37.0
8	32.70102884434633	36.0	33.0	37.0	25.0	37.0
9	34.05846365089284	38.0	34.0	39.0	25.0	39.0
10	33.91647391778904	38.0	34.0	39.0	25.0	39.0
11	33.96187398718607	38.0	34.0	39.0	25.0	39.0
12	33.82158648734396	38.0	34.0	39.0	23.0	39.0
13	33.79783843867094	38.0	34.0	39.0	23.0	39.0
14	34.7386897336239	39.0	34.0	41.0	22.0	41.0
15	34.72430954045646	39.0	34.0	41.0	22.0	41.0
16	34.70269689920119	39.0	34.0	41.0	22.0	41.0
17	34.68745704480121	39.0	34.0	41.0	22.0	41.0
18	34.62662913976665	39.0	34.0	41.0	22.0	41.0
19	34.654794376017456	39.0	34.0	41.0	21.0	41.0
20	34.58784184908153	39.0	34.0	41.0	19.0	41.0
21	34.516012955102205	39.0	34.0	41.0	18.0	41.0
22	34.43733872064279	39.0	34.0	41.0	18.0	41.0
23	34.305582448347884	39.0	34.0	41.0	18.0	41.0
24	34.2173768964372	39.0	34.0	41.0	16.0	41.0
25	34.07990985816704	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	48961.0
3	3.0
4	799.0
5	6576.0
6	7245.0
7	5452.0
8	4266.0
9	3589.0
10	3153.0
11	2982.0
12	2745.0
13	2617.0
14	2607.0
15	2520.0
16	2597.0
17	2845.0
18	3002.0
19	3647.0
20	4414.0
21	5894.0
22	7110.0
23	8722.0
24	10253.0
25	11899.0
26	13443.0
27	15283.0
28	17465.0
29	21095.0
30	26903.0
31	34015.0
32	42848.0
33	55411.0
34	73134.0
35	96249.0
36	129667.0
37	194953.0
38	471515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.4647983595352	18.794240522663866	9.408425604519758	31.332535513281172
2	15.207162010849414	21.005082923502037	35.14528423431824	28.642470831330307
3	18.198921014644647	24.242309367608737	27.851439563886547	29.70733005386007
4	13.781116533905266	16.31229347022068	32.5009571458839	37.40563284999015
5	14.784459747826306	37.5819549632476	32.33297098916982	15.300614299756274
6	34.2389127696806	34.384476205691016	16.39546591073744	14.981145113890943
7	29.56665628898596	32.0911076155582	19.447600266599892	18.894635828855947
8	27.73914355114715	34.63594124889565	18.692047112043056	18.932868087914144
9	27.285878597667534	15.663450907664325	18.813156080221596	38.237514414446544
10	17.12135228011517	28.727036314665185	30.780421658772173	23.37118974644748
11	35.6827434420681	22.715117181956185	21.603744353784666	19.998395022191048
12	23.672463139249082	24.76305395147844	28.46725849929711	23.09722440997536
13	28.562045075596586	21.205310039618734	25.280050645401936	24.952594239382744
14	22.890841641204066	20.768710365694997	25.4823025626157	30.85814543048524
15	24.673831534684407	28.105670036052256	22.679326697618144	24.541171731645196
16	24.704289630093616	27.180313665872358	24.0622308810865	24.053165822947527
17	23.32188584811451	27.23389426506253	25.614850325740647	23.829369561082313
18	23.48888514063267	26.964336002603634	26.403038762500824	23.143740094262867
19	24.58407947630052	26.152280039530098	26.224504201930436	23.03913628223895
20	24.907697668906394	25.803191888540244	25.724802708179002	23.564307734374363
21	24.84493465594331	25.433540186204738	25.8350344015819	23.88649075627005
22	25.013095920091484	25.143274938532766	25.630628923667693	24.21300021770806
23	24.70454925417495	25.15691118830457	25.75082198651385	24.387717571006633
24	24.22020243751291	25.706904007882315	25.86443136339926	24.208462191205516
25	24.178717208978288	25.395382833596823	26.009819070671703	24.416080886753186
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	554.0
1	554.0
2	589.0
3	624.0
4	624.0
5	624.0
6	807.0
7	990.0
8	990.0
9	990.0
10	1315.5
11	1641.0
12	1641.0
13	1641.0
14	2040.5
15	2440.0
16	2440.0
17	2440.0
18	4968.5
19	7497.0
20	7497.0
21	7497.0
22	12671.0
23	17845.0
24	17845.0
25	17845.0
26	27216.5
27	36588.0
28	36588.0
29	36588.0
30	48137.5
31	59687.0
32	59687.0
33	59687.0
34	73130.0
35	86573.0
36	86573.0
37	86573.0
38	103181.0
39	119789.0
40	119789.0
41	119789.0
42	136020.0
43	152251.0
44	152251.0
45	152251.0
46	167738.5
47	183226.0
48	183226.0
49	183226.0
50	189416.5
51	195607.0
52	195607.0
53	195607.0
54	184263.5
55	172920.0
56	172920.0
57	172920.0
58	152563.5
59	132207.0
60	132207.0
61	132207.0
62	110833.0
63	89459.0
64	89459.0
65	89459.0
66	69419.0
67	49379.0
68	49379.0
69	49379.0
70	36389.5
71	23400.0
72	23400.0
73	23400.0
74	16039.5
75	8679.0
76	8679.0
77	8679.0
78	5893.5
79	3108.0
80	3108.0
81	3108.0
82	2056.5
83	1005.0
84	1005.0
85	1005.0
86	644.0
87	283.0
88	283.0
89	283.0
90	179.5
91	76.0
92	76.0
93	76.0
94	58.5
95	41.0
96	41.0
97	41.0
98	25.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10268382224553618
2	0.0
3	0.040196778462254036
4	0.05453684915211546
5	0.06932272514839743
6	0.005498265445853602
7	0.003120637144943936
8	0.004903858370626186
9	5.201061908239894E-4
10	2.972035376137082E-4
11	0.004903858370626186
12	2.2290265321028114E-4
13	0.00378934510457478
14	0.057137380106235404
15	0.024667893621937782
16	0.003863645988978207
17	0.0017089203412788223
18	0.00505246013943304
19	0.005126761023836466
20	0.0022290265321028117
21	0.01159093796693462
22	0.002972035376137082
23	0.004012247757785061
24	0.0060183716366775915
25	0.17616739692052555
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1345879.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.75778239732569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.7787731319597	27.697966855420614
2	14.79529853613763	12.652283106232884
3	7.080930237742169	9.082946228280699
4	4.043789119120735	6.91613820864151
5	2.510557094211283	5.367292696517417
6	1.5978841622016122	4.099318998212977
7	1.1035156052033714	3.3028716083537244
8	0.7936328687155797	2.714718520312489
9	0.5616905183986196	2.161497686429632
>10	2.588867977861736	18.51303233460256
>50	0.10407039919705541	3.014504993793533
>100	0.037136569708011186	2.8249194190569855
>500	0.0024524051634171955	0.6445108510342021
>1k	0.0014013743790955405	1.0079984931107835
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2760	0.20507044095345867	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1890	0.14042867152247715	No Hit
CTTATACACATCTCCGAGCCCACGA	1739	0.12920923797755965	No Hit
GGTATCAACGCAGAGTACATGGGGG	1708	0.1269059105610534	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1645	0.1222249548436375	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	240	0.0	14.66736	1
TTGCGCG	40	0.005301478	14.24072	18
GGTATCA	985	0.0	13.81215	1
CGTGTCA	50	0.0014991653	13.300235	10
GGGCGGT	50	0.001508009	13.289857	6
TTATACA	315	0.0	12.371277	2
TATACAC	315	0.0	12.371277	3
AGTAGTC	55	0.0030693812	12.090222	5
ATGGTCG	55	0.0030779387	12.08573	15
TCGATAA	55	0.0030830828	12.0830345	18
ATTTGCG	55	0.0030830828	12.0830345	16
GTGTTAG	105	2.6672933E-7	11.779192	1
GCCCTAT	90	7.3373867E-6	11.628179	1
GTCGTAG	60	0.005811076	11.099624	1
TTACACT	60	0.005853087	11.088888	4
TCTATAC	60	0.005853087	11.088888	3
GTGTTAA	70	0.0014730139	10.873101	1
GAATAGG	105	3.404035E-6	10.8731	1
CCCTATA	70	0.0014849546	10.862585	2
ATAGAAC	70	0.0014849546	10.862585	3
>>END_MODULE