FastQCFastQC Report
Mon 15 Aug 2016
SRR2921170_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921170_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212327
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG400.00529168114.2452694
GTGTTAT1207.385097E-1012.6696231
GGTATCA10900.012.466211
GTACTAG853.9351653E-612.2969871
CCGTACA550.003068987312.0909952
TACACCG550.003076792212.0868965
TTCGCCG600.005882614311.08190915
ACCGTCC1158.866573E-710.7348388
GCGAATC909.530742E-510.55419915
GTATCAA23350.010.3364581
GCGTAGA1201.5197656E-610.2940681
CGTACAC951.6500553E-49.996683
GTATTAG2151.0913936E-119.7231981
TAGACAG3750.09.6234715
TGTACCG2002.6739144E-109.4968465
CTGATCG1002.7671104E-49.4962029
TACCGTA800.0045316469.4962027
CTGCTAG1856.672053E-99.24541
ATATAAC1554.281883E-79.1904963
CCGTATG1151.1157005E-49.08332359
Produced by FastQC (version 0.11.5)