##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921170_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2212327
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.504688050184264	31.0	26.0	31.0	7.0	34.0
2	26.062761969636497	31.0	26.0	31.0	7.0	34.0
3	25.939143264083473	31.0	26.0	31.0	7.0	34.0
4	29.71065579365076	35.0	30.0	37.0	7.0	37.0
5	29.37876769573395	35.0	28.0	37.0	2.0	37.0
6	29.662096064460634	35.0	30.0	37.0	2.0	37.0
7	29.685907643851927	35.0	30.0	37.0	2.0	37.0
8	29.373401400425887	35.0	29.0	37.0	2.0	37.0
9	30.900552223970507	37.0	30.0	39.0	2.0	39.0
10	30.248765666196725	35.0	28.0	39.0	2.0	39.0
11	30.67950578734518	37.0	30.0	39.0	2.0	39.0
12	30.397300218276953	37.0	29.0	39.0	2.0	39.0
13	30.382041172032885	37.0	30.0	39.0	2.0	39.0
14	31.419367028472735	37.0	30.0	40.0	2.0	41.0
15	31.056393562072877	37.0	29.0	40.0	2.0	41.0
16	30.985055102613675	37.0	29.0	40.0	2.0	41.0
17	31.277752339504964	37.0	30.0	40.0	2.0	41.0
18	31.33136059904345	38.0	30.0	40.0	2.0	41.0
19	31.04792148719425	38.0	30.0	40.0	2.0	41.0
20	30.89073134306095	37.0	29.0	40.0	2.0	41.0
21	30.84624108461362	37.0	30.0	40.0	2.0	41.0
22	30.100067485502823	37.0	27.0	40.0	2.0	41.0
23	30.15886213927688	37.0	27.0	40.0	2.0	41.0
24	29.758735485305742	37.0	27.0	40.0	2.0	41.0
25	29.28452484646257	37.0	26.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	214385.0
3	9.0
4	1594.0
5	9317.0
6	11204.0
7	9741.0
8	8331.0
9	7797.0
10	7448.0
11	6986.0
12	6799.0
13	6611.0
14	6506.0
15	6970.0
16	7146.0
17	8144.0
18	9149.0
19	11361.0
20	14089.0
21	17852.0
22	21731.0
23	25603.0
24	29564.0
25	33168.0
26	37255.0
27	41208.0
28	45559.0
29	53236.0
30	62086.0
31	73527.0
32	90541.0
33	113050.0
34	142222.0
35	178939.0
36	228312.0
37	290371.0
38	374516.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.55946155533098	17.82876754139056	11.963437490815286	25.648333412463174
2	16.138322274401407	20.05396204024631	38.30228511060138	25.505430574750903
3	18.9995732322593	23.27091252346318	29.915152786467846	27.814361457809667
4	13.75320343151991	15.557884182360409	35.23315827946626	35.45575410665342
5	14.102004245731663	36.57350648974912	34.13433545619917	15.190153808320042
6	33.86882212204534	34.393206373973975	16.965080375300197	14.772891128680488
7	29.491125490736742	31.262953528897246	20.715475525882194	18.53044545448381
8	26.351539796399447	34.62036556971043	20.32778052256661	18.700314111323515
9	26.737230385342997	15.786473554426959	19.204180482192946	38.272115578037095
10	15.914076579987151	27.9587876409934	33.12185924821302	23.00527653080643
11	35.57357262329913	22.73459608516794	22.06731160435785	19.62451968717508
12	23.314478108998657	24.75847818313572	29.341188245297833	22.585855462567793
13	28.693953643120558	21.219343062063828	25.252085987246748	24.834617307568863
14	22.423997750425638	20.90476651172211	25.92837890499617	30.74285683285608
15	24.52723148457044	28.085076182701062	22.93179313456126	24.455899198167238
16	24.3854488170178	27.327146599723097	24.614376649813213	23.67302793344589
17	23.205390475777044	27.361973288186608	25.730378537940613	23.702257698095735
18	23.16582957739538	27.073316180124067	26.336189338154682	23.424664904325873
19	24.298307438653186	26.53178858873956	26.594583206937877	22.57532076566938
20	24.803547936473493	25.883751734863175	26.21092319585532	23.101777132808017
21	25.145988371567306	25.713394399182572	25.621072239151466	23.51954499009865
22	24.30023777450298	25.160158757424806	26.761836740229093	23.777766727843122
23	24.33184852716662	25.585894145511606	26.027764503985377	24.054492823336396
24	24.075716601613532	25.87460070758278	26.058141858322507	23.991540832481178
25	23.98727779508422	25.5345496686396	26.595565937404352	23.88260659887182
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	912.0
1	912.0
2	978.0
3	1044.0
4	1044.0
5	1044.0
6	1282.0
7	1520.0
8	1520.0
9	1520.0
10	2226.5
11	2933.0
12	2933.0
13	2933.0
14	4844.0
15	6755.0
16	6755.0
17	6755.0
18	11942.0
19	17129.0
20	17129.0
21	17129.0
22	26754.0
23	36379.0
24	36379.0
25	36379.0
26	53124.5
27	69870.0
28	69870.0
29	69870.0
30	91098.0
31	112326.0
32	112326.0
33	112326.0
34	135894.0
35	159462.0
36	159462.0
37	159462.0
38	185322.5
39	211183.0
40	211183.0
41	211183.0
42	233730.0
43	256277.0
44	256277.0
45	256277.0
46	274913.5
47	293550.0
48	293550.0
49	293550.0
50	296432.0
51	299314.0
52	299314.0
53	299314.0
54	281326.5
55	263339.0
56	263339.0
57	263339.0
58	233696.0
59	204053.0
60	204053.0
61	204053.0
62	170749.5
63	137446.0
64	137446.0
65	137446.0
66	107680.5
67	77915.0
68	77915.0
69	77915.0
70	57571.5
71	37228.0
72	37228.0
73	37228.0
74	26246.0
75	15264.0
76	15264.0
77	15264.0
78	10489.5
79	5715.0
80	5715.0
81	5715.0
82	3836.0
83	1957.0
84	1957.0
85	1957.0
86	1274.0
87	591.0
88	591.0
89	591.0
90	361.0
91	131.0
92	131.0
93	131.0
94	76.5
95	22.0
96	22.0
97	22.0
98	17.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034895383910244736
2	0.05166505674794006
3	0.015865647347792618
4	0.011119513525803372
5	0.010079884212415253
6	8.136229409124419E-4
7	0.004158517253552481
8	0.002440868822737326
9	0.0064185809783092644
10	0.0035256994106205816
11	0.010260689310395796
12	0.009401865094988218
13	0.008362235781600098
14	0.015956049896782887
15	0.006554184801794672
16	0.03222850871503173
17	0.03937031008526316
18	0.022419832149587288
19	0.016001251171278028
20	0.015232829504860717
21	0.022917046169033783
22	0.006644587350784943
23	0.016543666465219653
24	0.013063168329094207
25	0.46073659092891783
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2212327.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.58058092649721
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.83337645555518	40.29868578708964
2	12.308159326324185	13.435729723333173
3	5.326433700421901	8.721595369064989
4	2.795090383487696	6.102306274912973
5	1.632632539141429	4.455501621292069
6	1.04194043490516	3.412182853675847
7	0.6830514672522872	2.6096942119728284
8	0.4893045907405931	2.1365223050098834
9	0.3490937939670952	1.7148367865303087
>10	1.474846581020392	13.378248601710657
>50	0.04863770087959575	1.787541832349496
>100	0.016323318930037738	1.4430851630207109
>500	8.536210572366681E-4	0.3203606318492757
>1k	2.5608631717095816E-4	0.1837088381881231
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8080509798054265E-4
2	0.0	0.0	0.0	0.0	1.8080509798054265E-4
3	0.0	0.0	0.0	0.0	1.8080509798054265E-4
4	0.0	0.0	0.0	0.0	1.8080509798054265E-4
5	0.0	0.0	0.0	0.0	1.8080509798054265E-4
6	0.0	0.0	0.0	0.0	2.2600637247567832E-4
7	0.0	0.0	0.0	0.0	2.2600637247567832E-4
8	0.0	0.0	0.0	0.0	2.71207646970814E-4
9	0.0	0.0	0.0	4.520127449513566E-5	3.1640892146594967E-4
10	0.0	0.0	0.0	4.520127449513566E-5	4.06811470456221E-4
11	0.0	0.0	0.0	4.520127449513566E-5	4.06811470456221E-4
12	0.0	0.0	0.0	4.520127449513566E-5	8.13622940912442E-4
13	0.0	0.0	0.0	4.520127449513566E-5	9.49226764397849E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	40	0.005291681	14.245269	4
GTGTTAT	120	7.385097E-10	12.669623	1
GGTATCA	1090	0.0	12.46621	1
GTACTAG	85	3.9351653E-6	12.296987	1
CCGTACA	55	0.0030689873	12.090995	2
TACACCG	55	0.0030767922	12.086896	5
TTCGCCG	60	0.0058826143	11.081909	15
ACCGTCC	115	8.866573E-7	10.734838	8
GCGAATC	90	9.530742E-5	10.554199	15
GTATCAA	2335	0.0	10.336458	1
GCGTAGA	120	1.5197656E-6	10.294068	1
CGTACAC	95	1.6500553E-4	9.99668	3
GTATTAG	215	1.0913936E-11	9.723198	1
TAGACAG	375	0.0	9.623471	5
TGTACCG	200	2.6739144E-10	9.496846	5
CTGATCG	100	2.7671104E-4	9.496202	9
TACCGTA	80	0.004531646	9.496202	7
CTGCTAG	185	6.672053E-9	9.2454	1
ATATAAC	155	4.281883E-7	9.190496	3
CCGTATG	115	1.1157005E-4	9.0833235	9
>>END_MODULE