FastQCFastQC Report
Mon 15 Aug 2016
SRR2921170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212327
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39470.17840943043230048No Hit
TATCAACGCAGAGTACTTTTTTTTT23560.10649420271053962No Hit
GGTATCAACGCAGAGTACTTTTTTT23050.1041889377112877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTT456.789731E-414.77035815
ACGGATT400.0052794114.25025513
ACGGTAT500.001499695213.30023811
GGTATCA12600.012.5331051
ATCGGAC855.2958723E-511.18248753
TATCGGA855.2958723E-511.18248752
CGTGCGC855.324017E-511.1766710
GACGGTT855.360928E-511.16909218
CGCCGAA600.005876209611.08353112
GTATCAA27900.010.6382831
CGGACTG1201.524415E-610.2916175
GACAGTC2107.2759576E-129.9451367
GCTCTAA1151.0101145E-59.9266921
TCGGACT1356.399805E-79.8569334
GTATAAA1752.5138434E-99.7848821
GACTAGA800.0044657469.5130811
GTACTAG1106.736035E-59.5130791
CTAAGAC2401.8189894E-129.5051153
TAAAACG900.00110866469.5048994
TTCGCCT1606.705159E-89.49372917
Produced by FastQC (version 0.11.5)