##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921170_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2212327
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.009114837001945	31.0	30.0	33.0	26.0	34.0
2	30.243063073406418	31.0	30.0	34.0	27.0	34.0
3	30.371061330445272	31.0	30.0	34.0	27.0	34.0
4	34.08741610078438	35.0	35.0	37.0	30.0	37.0
5	33.736911405954004	35.0	35.0	37.0	28.0	37.0
6	33.73045756798159	37.0	35.0	37.0	29.0	37.0
7	33.60206470381639	37.0	35.0	37.0	28.0	37.0
8	33.56324042512703	37.0	35.0	37.0	28.0	37.0
9	35.001705896099445	39.0	35.0	39.0	28.0	39.0
10	34.876118223029415	39.0	35.0	39.0	28.0	39.0
11	34.9330980456325	39.0	35.0	39.0	28.0	39.0
12	34.812138079045276	39.0	35.0	39.0	27.0	39.0
13	34.8049736770378	39.0	35.0	39.0	27.0	39.0
14	35.80598618558649	39.0	36.0	41.0	27.0	41.0
15	35.809593699303946	39.0	36.0	41.0	27.0	41.0
16	35.79228613129976	39.0	36.0	41.0	27.0	41.0
17	35.776561963941134	39.0	36.0	41.0	27.0	41.0
18	35.726330239607435	39.0	36.0	41.0	27.0	41.0
19	35.77002585964914	40.0	36.0	41.0	27.0	41.0
20	35.70941095055116	40.0	36.0	41.0	27.0	41.0
21	35.648978654602146	39.0	36.0	41.0	27.0	41.0
22	35.569791445839606	39.0	36.0	41.0	27.0	41.0
23	35.45846793896201	39.0	36.0	41.0	27.0	41.0
24	35.3935629769017	39.0	36.0	41.0	27.0	41.0
25	35.265495109900115	39.0	35.0	41.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	52598.0
3	10.0
4	785.0
5	6711.0
6	7939.0
7	6103.0
8	4824.0
9	4151.0
10	3674.0
11	3497.0
12	3272.0
13	3214.0
14	3062.0
15	3056.0
16	3200.0
17	3280.0
18	3916.0
19	4571.0
20	5656.0
21	7451.0
22	9622.0
23	11762.0
24	14279.0
25	16763.0
26	19361.0
27	22155.0
28	25980.0
29	31999.0
30	41025.0
31	53438.0
32	69150.0
33	90507.0
34	120943.0
35	160979.0
36	222200.0
37	337353.0
38	833841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.70642659703423	19.063509436984436	9.350019297935235	30.880044668046104
2	15.31979675698936	20.908663140665915	35.20026650671442	28.571273595630302
3	18.234052145742545	23.750881237454095	27.983534256226545	30.03153236057682
4	13.418917873845565	16.08703537123493	33.79124047383553	36.70280628108398
5	14.557896422300443	37.36596856791604	32.951581781223794	15.12455322855972
6	34.20865515833858	35.295691767623325	16.212476601907646	14.28317647213045
7	30.002870296499367	31.64626194732667	19.606746778826704	18.744120977347258
8	27.4759906810483	35.10599539115098	18.63388037424071	18.784133553560014
9	26.96252722247987	15.576366231739808	18.93220076968765	38.52890577609268
10	16.51681690816954	28.58682283405663	31.463929156946506	23.43243110082732
11	36.033359324677086	22.474827948604943	21.77138918082065	19.720423545897322
12	23.55858785794324	24.776581400489167	28.874393342394683	22.79043739917291
13	28.847158713886135	20.954044373568237	25.205757968601116	24.993038943944516
14	22.66671309866181	20.561760809798116	25.8438826675543	30.927643423985774
15	24.72332664500722	28.099876391052774	22.50012885351119	24.676668110428814
16	24.80938493304742	27.24186281139369	24.01469593800513	23.93405631755376
17	23.351990963336693	27.152976444195936	25.741336813238224	23.753695779229144
18	23.2445963849785	26.877924881648664	26.415503720388433	23.461975012984407
19	24.434075995347758	26.30464586779082	26.38641719105334	22.874860945808077
20	24.818064294496274	25.746094597526582	26.086370868590443	23.349470239386708
21	24.992450735397824	25.507925371676965	25.80374805165686	23.69587584126835
22	24.84023544535641	25.316930025430338	25.576657605280978	24.266176923932274
23	24.412749971409884	25.44461585958162	25.81034333904533	24.332290829963167
24	24.07251792629505	25.858163600636995	25.912000119335353	24.1573183537326
25	24.20762837536479	25.58025596964407	26.03650046525528	24.175615189735858
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	425.0
1	425.0
2	478.0
3	531.0
4	531.0
5	531.0
6	818.5
7	1106.0
8	1106.0
9	1106.0
10	1588.0
11	2070.0
12	2070.0
13	2070.0
14	3297.0
15	4524.0
16	4524.0
17	4524.0
18	9394.5
19	14265.0
20	14265.0
21	14265.0
22	23552.5
23	32840.0
24	32840.0
25	32840.0
26	49345.5
27	65851.0
28	65851.0
29	65851.0
30	85940.5
31	106030.0
32	106030.0
33	106030.0
34	128437.0
35	150844.0
36	150844.0
37	150844.0
38	176473.0
39	202102.0
40	202102.0
41	202102.0
42	224781.0
43	247460.0
44	247460.0
45	247460.0
46	269359.0
47	291258.0
48	291258.0
49	291258.0
50	299395.5
51	307533.0
52	307533.0
53	307533.0
54	290110.5
55	272688.0
56	272688.0
57	272688.0
58	243497.5
59	214307.0
60	214307.0
61	214307.0
62	180933.5
63	147560.0
64	147560.0
65	147560.0
66	116101.0
67	84642.0
68	84642.0
69	84642.0
70	62645.5
71	40649.0
72	40649.0
73	40649.0
74	28469.5
75	16290.0
76	16290.0
77	16290.0
78	11236.5
79	6183.0
80	6183.0
81	6183.0
82	4190.5
83	2198.0
84	2198.0
85	2198.0
86	1448.5
87	699.0
88	699.0
89	699.0
90	443.0
91	187.0
92	187.0
93	187.0
94	130.0
95	73.0
96	73.0
97	73.0
98	42.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10152206251607471
2	0.0
3	0.04285080822138861
4	0.053337503904260085
5	0.06621986713537376
6	0.003344894312640039
7	5.42415293941628E-4
8	0.0027572777442032756
9	4.520127449513566E-5
10	0.0
11	0.0023504662737470544
12	0.0
13	8.588242154075776E-4
14	0.05460313959012389
15	0.023188253816004596
16	6.78019117427035E-4
17	2.71207646970814E-4
18	0.0026216739207178684
19	0.00271207646970814
20	3.1640892146594967E-4
21	0.008633443428570912
22	4.0681147045622094E-4
23	0.001084830587883256
24	0.003344894312640039
25	0.17456732210021395
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2212327.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.63766809315113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.863363075578626	24.124817846227355
2	16.157475339800573	12.808892894845789
3	7.881906358624029	9.3726116455331
4	4.415473970848107	7.000763669217013
5	2.7389705194489777	5.428320218342216
6	1.8096865519908276	4.30390529402709
7	1.2611406566053893	3.499207233571218
8	0.9169853301805959	2.907772813118965
9	0.6669039850328944	2.3791066927860323
>10	3.112693925379129	20.69077661768561
>50	0.12980124882349256	3.4732768924081094
>100	0.04306773466283987	2.8835130627274315
>500	0.0014957699690609564	0.3660857439425496
>1k	0.0010355330555037008	0.7609493755676155
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3947	0.17840943043230048	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2356	0.10649420271053962	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2305	0.1041889377112877	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.35603823485407E-4
2	0.0	0.0	0.0	0.0	1.35603823485407E-4
3	0.0	0.0	0.0	0.0	1.35603823485407E-4
4	0.0	0.0	0.0	0.0	1.8080509798054265E-4
5	0.0	0.0	0.0	0.0	1.8080509798054265E-4
6	0.0	0.0	0.0	0.0	1.8080509798054265E-4
7	0.0	0.0	0.0	0.0	2.2600637247567832E-4
8	0.0	0.0	0.0	0.0	3.616101959610853E-4
9	0.0	0.0	0.0	4.520127449513566E-5	4.5201274495135663E-4
10	0.0	0.0	0.0	4.520127449513566E-5	5.42415293941628E-4
11	0.0	0.0	0.0	4.520127449513566E-5	5.876165684367636E-4
12	0.0	0.0	0.0	4.520127449513566E-5	0.001220434411368663
13	0.0	0.0	0.0	4.520127449513566E-5	0.0014464407838443412
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTT	45	6.789731E-4	14.770358	15
ACGGATT	40	0.00527941	14.250255	13
ACGGTAT	50	0.0014996952	13.300238	11
GGTATCA	1260	0.0	12.533105	1
ATCGGAC	85	5.2958723E-5	11.1824875	3
TATCGGA	85	5.2958723E-5	11.1824875	2
CGTGCGC	85	5.324017E-5	11.17667	10
GACGGTT	85	5.360928E-5	11.169092	18
CGCCGAA	60	0.0058762096	11.083531	12
GTATCAA	2790	0.0	10.638283	1
CGGACTG	120	1.524415E-6	10.291617	5
GACAGTC	210	7.2759576E-12	9.945136	7
GCTCTAA	115	1.0101145E-5	9.926692	1
TCGGACT	135	6.399805E-7	9.856933	4
GTATAAA	175	2.5138434E-9	9.784882	1
GACTAGA	80	0.004465746	9.513081	1
GTACTAG	110	6.736035E-5	9.513079	1
CTAAGAC	240	1.8189894E-12	9.505115	3
TAAAACG	90	0.0011086646	9.504899	4
TTCGCCT	160	6.705159E-8	9.493729	17
>>END_MODULE