FastQCFastQC Report
Mon 15 Aug 2016
SRR2921169_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921169_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306342
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG500.00150318613.2961755
GGTATCA11900.012.8537041
GCGTAGG658.022007E-411.6930021
TCTATAC1501.7826096E-1011.3964743
GTATCAA25300.010.5520111
CTAAGAC1701.5679689E-910.0557123
GTGCTAG2750.010.0187761
AACCGCT1304.2591364E-69.496657
GTATAAT2550.09.3142781
GTATTAT1951.6807462E-99.2569611
GCCTTAG1651.0650183E-79.2126681
GTGGTAT3450.09.0874961
GCACCGT1151.1151829E-49.0837526
AACCGTC850.00745858448.9380237
GTACTAC1606.7367E-78.9067791
ACCGTGC1606.774753E-78.9033028
CCTAGAC2054.1636667E-98.8021553
GTATTAG1951.6474587E-88.76975251
GTATATA3150.08.74655151
TTTATAC1856.608752E-88.727033
Produced by FastQC (version 0.11.5)