##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921169_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2306342
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.356388167929996	30.0	26.0	31.0	7.0	33.0
2	26.10947075498777	31.0	26.0	31.0	7.0	34.0
3	25.85958804028197	30.0	25.0	31.0	7.0	34.0
4	29.615804160874667	35.0	28.0	37.0	7.0	37.0
5	29.378952471055896	35.0	28.0	37.0	7.0	37.0
6	29.97772099714613	35.0	30.0	37.0	2.0	37.0
7	29.969661481254732	35.0	30.0	37.0	2.0	37.0
8	29.594381926010975	35.0	30.0	37.0	2.0	37.0
9	31.181477855409128	37.0	30.0	39.0	2.0	39.0
10	30.488888464937116	37.0	29.0	39.0	2.0	39.0
11	30.96895820307656	37.0	30.0	39.0	2.0	39.0
12	30.659968035963445	37.0	30.0	39.0	2.0	39.0
13	30.660356529950892	37.0	30.0	39.0	2.0	39.0
14	31.69893190168674	38.0	30.0	40.0	2.0	41.0
15	31.346198438913223	38.0	30.0	40.0	2.0	41.0
16	31.262628005733756	37.0	30.0	40.0	2.0	41.0
17	31.561849456845515	38.0	30.0	40.0	2.0	41.0
18	31.619304075458018	38.0	31.0	40.0	2.0	41.0
19	31.339088912225506	38.0	30.0	40.0	2.0	41.0
20	31.18755717929084	38.0	30.0	40.0	2.0	41.0
21	31.140177822716666	38.0	30.0	40.0	2.0	41.0
22	30.411603309483155	37.0	27.0	40.0	2.0	41.0
23	30.487470201730705	37.0	28.0	40.0	2.0	41.0
24	30.096565036755173	37.0	27.0	40.0	2.0	41.0
25	29.62453226798107	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	218162.0
3	17.0
4	1630.0
5	8702.0
6	10553.0
7	9601.0
8	8408.0
9	7701.0
10	7396.0
11	7096.0
12	6919.0
13	6683.0
14	6716.0
15	6783.0
16	7422.0
17	7941.0
18	9233.0
19	11295.0
20	14321.0
21	18161.0
22	21936.0
23	25558.0
24	29612.0
25	33338.0
26	37423.0
27	41546.0
28	46354.0
29	53933.0
30	63057.0
31	74911.0
32	92818.0
33	116137.0
34	146703.0
35	187912.0
36	244264.0
37	319726.0
38	396374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.60708154525053	17.96492972151451	11.785515076281412	25.642473656953552
2	16.393877148193056	19.809815910998704	38.47605454778137	25.32025239302687
3	19.52416615459071	23.379458475879815	29.558498733506422	27.537876636023057
4	13.730497376523134	15.869042972984692	34.82143012011621	35.57902953037596
5	14.372892821445685	36.744597492308465	34.01532020441393	14.867189481831918
6	34.1718238567211	34.191638913302405	17.13226268434615	14.504274545630347
7	29.639326235860807	31.49418519191403	20.23503783386746	18.631450738357703
8	26.816285825781556	33.88869618002862	20.363395915535705	18.93162207865412
9	27.391271543406827	15.45414494583632	18.996053175888207	38.158530334868644
10	15.960016598323604	27.962018109863727	32.8021551804504	23.275810111362265
11	35.953327944604794	22.400704582038188	22.05938471358792	19.586582759769094
12	23.696861053438585	24.51923660653113	29.082564510841568	22.701337829188716
13	28.737053667251335	21.18929560242048	25.31616478510768	24.75748594522051
14	22.9375084834479	20.612534210249002	25.748657501145942	30.701299805157156
15	24.729618044022544	27.911630565150542	22.771966826555754	24.586784564271163
16	24.442210244481526	27.08398302721276	24.60065241916001	23.873154309145708
17	23.50694507702438	27.103411420446317	25.69713200547937	23.692511497049935
18	23.423044429371163	26.925472249641334	26.299217789096236	23.35226553189127
19	24.719507012324694	26.162996435293202	26.45038465875088	22.66711189363123
20	25.01818115586617	25.60310048877712	25.965509360747525	23.41320899460918
21	25.435528282828724	25.36722117581035	25.548549438949276	23.648701102411653
22	24.449020492921072	24.950449026563483	26.787097971521252	23.81343250899419
23	24.70534664972484	25.238363697076664	25.989513992185366	24.066775661013125
24	24.270105041042022	25.59136810070549	26.050768174684773	24.08775868356771
25	24.05476274805276	25.355652472805733	26.51905155855398	24.070533220587524
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	702.0
1	702.0
2	787.5
3	873.0
4	873.0
5	873.0
6	1253.5
7	1634.0
8	1634.0
9	1634.0
10	2196.0
11	2758.0
12	2758.0
13	2758.0
14	4544.5
15	6331.0
16	6331.0
17	6331.0
18	11320.0
19	16309.0
20	16309.0
21	16309.0
22	25931.0
23	35553.0
24	35553.0
25	35553.0
26	52374.0
27	69195.0
28	69195.0
29	69195.0
30	90751.0
31	112307.0
32	112307.0
33	112307.0
34	136302.5
35	160298.0
36	160298.0
37	160298.0
38	188056.5
39	215815.0
40	215815.0
41	215815.0
42	242242.5
43	268670.0
44	268670.0
45	268670.0
46	288428.0
47	308186.0
48	308186.0
49	308186.0
50	311764.0
51	315342.0
52	315342.0
53	315342.0
54	297534.0
55	279726.0
56	279726.0
57	279726.0
58	248193.5
59	216661.0
60	216661.0
61	216661.0
62	182173.0
63	147685.0
64	147685.0
65	147685.0
66	115381.0
67	83077.0
68	83077.0
69	83077.0
70	61521.5
71	39966.0
72	39966.0
73	39966.0
74	27988.0
75	16010.0
76	16010.0
77	16010.0
78	11071.0
79	6132.0
80	6132.0
81	6132.0
82	4159.5
83	2187.0
84	2187.0
85	2187.0
86	1429.0
87	671.0
88	671.0
89	671.0
90	436.5
91	202.0
92	202.0
93	202.0
94	119.5
95	37.0
96	37.0
97	37.0
98	26.0
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03468696316504664
2	0.052333955675264124
3	0.01599936175987776
4	0.01049280635742661
5	0.010276012837645067
6	6.503805593446245E-4
7	0.0032519027967231225
8	0.0018210655661649484
9	0.007978001527960727
10	0.0036854898362862058
11	0.012747458963154639
12	0.00945219746247521
13	0.008108077639829653
14	0.016519666207353463
15	0.006937392633009328
16	0.033342843342401084
17	0.04197122542970644
18	0.02471446125509573
19	0.01743019899043594
20	0.01660638361526608
21	0.025104689590702504
22	0.005679990218276388
23	0.01786378602999902
24	0.012617382851285715
25	0.4731735362751925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2306342.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.73885298260283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.23955600242279	44.01984515482805
2	12.264089644969658	14.162289379527298
3	4.691404957711774	8.126290234055226
4	2.39336470492434	5.527605313255083
5	1.2588437719083463	3.634209773714061
6	0.7675466832879941	2.659035906218995
7	0.5002538922875381	2.0218860158535494
8	0.3430667245946309	1.584662333967399
9	0.2605071354863268	1.3537244877087617
>10	1.1991033110741574	12.053229054884708
>50	0.06084770481754634	2.411665503260566
>100	0.02048571627275314	1.902690412192639
>500	5.42354307873178E-4	0.2481483387037674
>1k	3.8739593419512606E-4	0.29471809182989295
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.33587039563083E-5
2	0.0	0.0	0.0	0.0	4.33587039563083E-5
3	0.0	0.0	0.0	0.0	4.33587039563083E-5
4	0.0	0.0	0.0	0.0	4.33587039563083E-5
5	0.0	0.0	0.0	0.0	4.33587039563083E-5
6	0.0	0.0	0.0	0.0	4.33587039563083E-5
7	0.0	0.0	0.0	0.0	4.33587039563083E-5
8	0.0	0.0	0.0	0.0	4.33587039563083E-5
9	0.0	0.0	0.0	0.0	4.33587039563083E-5
10	0.0	0.0	0.0	0.0	8.67174079126166E-5
11	0.0	0.0	0.0	4.33587039563083E-5	8.67174079126166E-5
12	0.0	0.0	0.0	4.33587039563083E-5	1.300761118689249E-4
13	0.0	0.0	0.0	4.33587039563083E-5	1.734348158252332E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGACCG	50	0.001503186	13.296175	5
GGTATCA	1190	0.0	12.853704	1
GCGTAGG	65	8.022007E-4	11.693002	1
TCTATAC	150	1.7826096E-10	11.396474	3
GTATCAA	2530	0.0	10.552011	1
CTAAGAC	170	1.5679689E-9	10.055712	3
GTGCTAG	275	0.0	10.018776	1
AACCGCT	130	4.2591364E-6	9.49665	7
GTATAAT	255	0.0	9.314278	1
GTATTAT	195	1.6807462E-9	9.256961	1
GCCTTAG	165	1.0650183E-7	9.212668	1
GTGGTAT	345	0.0	9.087496	1
GCACCGT	115	1.1151829E-4	9.083752	6
AACCGTC	85	0.0074585844	8.938023	7
GTACTAC	160	6.7367E-7	8.906779	1
ACCGTGC	160	6.774753E-7	8.903302	8
CCTAGAC	205	4.1636667E-9	8.802155	3
GTATTAG	195	1.6474587E-8	8.7697525	1
GTATATA	315	0.0	8.7465515	1
TTTATAC	185	6.608752E-8	8.72703	3
>>END_MODULE