FastQCFastQC Report
Mon 15 Aug 2016
SRR2921169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306342
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35390.15344645330137507No Hit
GGTATCAACGCAGAGTACATGGGGG23390.10141600855380511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14350.013.5247791
TATACAC3000.012.3547273
TCGTAAA550.003060436812.0955352
TTGCGCG907.5215703E-611.60321518
GGACCGT1406.89397E-1011.5273696
CCGCCTA752.07229E-411.40139314
CGTAAAC855.3045005E-511.1807483
GTATCAA29450.010.2729161
GGTATAC750.00261680410.1480051
CGCCTAG856.6144444E-410.05568915
GCGAATC1054.120634E-59.9492815
TTTGCGC1054.1373583E-59.94561317
CTTACAC2201.8189894E-129.9356183
CTAGCCA2350.09.7058414
CTAGACA2450.09.6975874
TCCTAGG3350.09.6454812
ATAGCAC2002.6375346E-109.5036353
TAGGTCG900.00111601529.498075
CTTATAC3150.09.3627421
TAATACT3050.09.3478384
Produced by FastQC (version 0.11.5)