##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921169_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2306342
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.810539807192516	31.0	30.0	33.0	26.0	34.0
2	30.042685776870908	31.0	30.0	34.0	26.0	34.0
3	30.177661422286896	31.0	30.0	34.0	26.0	34.0
4	33.87860733577241	35.0	35.0	37.0	30.0	37.0
5	33.49465170386699	35.0	35.0	37.0	28.0	37.0
6	33.47510126425309	37.0	35.0	37.0	28.0	37.0
7	33.334989346766434	37.0	35.0	37.0	28.0	37.0
8	33.28082955606757	37.0	35.0	37.0	27.0	37.0
9	34.69209293331171	39.0	35.0	39.0	27.0	39.0
10	34.560345776992314	38.0	35.0	39.0	27.0	39.0
11	34.60942479476157	39.0	35.0	39.0	27.0	39.0
12	34.48055232051448	38.0	35.0	39.0	27.0	39.0
13	34.47194821930139	38.0	35.0	39.0	27.0	39.0
14	35.44372994117958	39.0	35.0	41.0	27.0	41.0
15	35.44069526548968	39.0	35.0	41.0	27.0	41.0
16	35.42500418411493	39.0	35.0	41.0	27.0	41.0
17	35.40255174644523	39.0	36.0	41.0	27.0	41.0
18	35.355606410497664	39.0	35.0	41.0	26.0	41.0
19	35.38725392851537	39.0	35.0	41.0	26.0	41.0
20	35.324791813182955	39.0	35.0	41.0	26.0	41.0
21	35.25872225368137	39.0	35.0	41.0	26.0	41.0
22	35.17568903484392	39.0	35.0	41.0	25.0	41.0
23	35.06378629015124	39.0	35.0	41.0	25.0	41.0
24	34.98855200139442	39.0	35.0	41.0	25.0	41.0
25	34.857528935431084	39.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	63570.0
3	10.0
4	1014.0
5	8320.0
6	9454.0
7	7445.0
8	5667.0
9	5005.0
10	4526.0
11	4104.0
12	3887.0
13	3762.0
14	3567.0
15	3597.0
16	3790.0
17	4018.0
18	4422.0
19	5120.0
20	6574.0
21	8618.0
22	10899.0
23	13332.0
24	15926.0
25	18865.0
26	21593.0
27	24352.0
28	28413.0
29	34725.0
30	44487.0
31	57375.0
32	72910.0
33	94610.0
34	125634.0
35	166279.0
36	227010.0
37	344517.0
38	848945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.92639257927617	18.789740081960595	9.184239468688396	31.099627870074837
2	15.256497084994333	20.77371872861874	35.21086638495071	28.758917801436212
3	18.190761308500882	23.847852809699333	27.990290950510044	29.971094931289738
4	13.492987283879973	16.07694833943377	33.30859031152347	37.12147406516279
5	14.714216112256235	37.400653639290546	32.65207222223909	15.233058026214119
6	34.322505339707746	34.96131909642519	16.27927950584181	14.436896058025258
7	30.044937155621998	31.725918037819362	19.54826972005765	18.68087508650099
8	27.789373912772014	34.8205629391187	18.67300518053838	18.717057967570906
9	27.58766913146446	15.283032611815594	18.66219320465048	38.46710505206946
10	16.700472002851267	28.55105617466967	31.170051969742563	23.5784198527365
11	36.38614752175642	22.25699606864756	21.707465233741384	19.649391175854635
12	23.859124102149636	24.681682074904764	28.55326746857144	22.905926354374156
13	28.85300121578333	20.984879887197508	25.224859213677824	24.93725968334134
14	23.074540466535137	20.373084084256043	25.57857054855967	30.97380490064915
15	24.826229491133333	27.917733374216258	22.313664279757184	24.94237285489322
16	24.86654133056299	27.07910222812674	23.93623294477985	24.118123496530423
17	23.585918456011218	27.002156665675198	25.59134870951266	23.82057616880093
18	23.587681039711995	26.679561606725137	26.238514766527445	23.494242587035423
19	24.843983664082618	26.033839924832925	26.08370271544086	23.038473695643596
20	25.08515671610728	25.50513021519848	25.73402140884936	23.67569165984488
21	25.328897304605235	25.16300150849837	25.648157823877288	23.859943363019113
22	25.044258569772882	25.140298473811022	25.518951513072917	24.296491443343175
23	24.77384001956353	25.136884021901444	25.67978070806805	24.409495250466975
24	24.288827454156646	25.66985152181039	25.758954044777592	24.28236697925537
25	24.363685952753418	25.32817848670913	25.81120649295262	24.496929067584833
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	495.0
1	495.0
2	519.5
3	544.0
4	544.0
5	544.0
6	839.5
7	1135.0
8	1135.0
9	1135.0
10	1583.5
11	2032.0
12	2032.0
13	2032.0
14	3075.5
15	4119.0
16	4119.0
17	4119.0
18	8673.0
19	13227.0
20	13227.0
21	13227.0
22	22263.0
23	31299.0
24	31299.0
25	31299.0
26	47111.5
27	62924.0
28	62924.0
29	62924.0
30	83335.5
31	103747.0
32	103747.0
33	103747.0
34	126663.0
35	149579.0
36	149579.0
37	149579.0
38	178055.5
39	206532.0
40	206532.0
41	206532.0
42	233915.0
43	261298.0
44	261298.0
45	261298.0
46	284160.5
47	307023.0
48	307023.0
49	307023.0
50	314877.5
51	322732.0
52	322732.0
53	322732.0
54	307346.0
55	291960.0
56	291960.0
57	291960.0
58	260310.5
59	228661.0
60	228661.0
61	228661.0
62	193831.5
63	159002.0
64	159002.0
65	159002.0
66	124491.5
67	89981.0
68	89981.0
69	89981.0
70	66437.0
71	42893.0
72	42893.0
73	42893.0
74	29841.5
75	16790.0
76	16790.0
77	16790.0
78	11761.5
79	6733.0
80	6733.0
81	6733.0
82	4617.0
83	2501.0
84	2501.0
85	2501.0
86	1646.5
87	792.0
88	792.0
89	792.0
90	525.5
91	259.0
92	259.0
93	259.0
94	162.5
95	66.0
96	66.0
97	66.0
98	42.0
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10007188873115956
2	0.0
3	0.03746192021825037
4	0.057016695702545424
5	0.06074554424278793
6	0.001560913342427099
7	4.33587039563083E-4
8	7.804566712135495E-4
9	0.0
10	0.0
11	4.7694574351939134E-4
12	0.0
13	4.33587039563083E-4
14	0.05107655326053118
15	0.024757819959052043
16	4.33587039563083E-4
17	1.734348158252332E-4
18	4.7694574351939134E-4
19	5.636631514320079E-4
20	2.601522237378498E-4
21	0.0032519027967231225
22	3.9022833560677474E-4
23	4.33587039563083E-4
24	4.33587039563083E-4
25	0.16406933577067062
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2306342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.983780259267135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.51431865306876	29.920654943845175
2	15.641146133620914	14.071957613557782
3	6.699583446844913	9.041177688044852
4	3.566052876856935	6.4165815602183915
5	2.0615478405511323	4.636810752665941
6	1.3097919173307029	3.5351635076741115
7	0.8847269600651818	2.7858854212715083
8	0.578751563464424	2.0827546524472758
9	0.4352081251834024	1.7619576003268016
>10	2.130908948811623	16.94625057846178
>50	0.12454718527058117	3.8475245287253443
>100	0.051378985007939984	3.8241300722480305
>500	9.701732969068919E-4	0.2874266229760554
>1k	0.0010671906265973207	0.8417244575369149
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3539	0.15344645330137507	No Hit
GGTATCAACGCAGAGTACATGGGGG	2339	0.10141600855380511	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.67174079126166E-5
2	0.0	0.0	0.0	0.0	8.67174079126166E-5
3	0.0	0.0	0.0	0.0	8.67174079126166E-5
4	0.0	0.0	0.0	0.0	8.67174079126166E-5
5	0.0	0.0	0.0	0.0	1.300761118689249E-4
6	0.0	0.0	0.0	0.0	1.734348158252332E-4
7	0.0	0.0	0.0	0.0	1.734348158252332E-4
8	0.0	0.0	0.0	0.0	1.734348158252332E-4
9	0.0	0.0	0.0	0.0	1.734348158252332E-4
10	0.0	0.0	0.0	0.0	1.734348158252332E-4
11	0.0	0.0	0.0	0.0	1.734348158252332E-4
12	0.0	0.0	0.0	0.0	3.902283356067747E-4
13	0.0	0.0	0.0	0.0	3.902283356067747E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1435	0.0	13.524779	1
TATACAC	300	0.0	12.354727	3
TCGTAAA	55	0.0030604368	12.095535	2
TTGCGCG	90	7.5215703E-6	11.603215	18
GGACCGT	140	6.89397E-10	11.527369	6
CCGCCTA	75	2.07229E-4	11.401393	14
CGTAAAC	85	5.3045005E-5	11.180748	3
GTATCAA	2945	0.0	10.272916	1
GGTATAC	75	0.002616804	10.148005	1
CGCCTAG	85	6.6144444E-4	10.055689	15
GCGAATC	105	4.120634E-5	9.94928	15
TTTGCGC	105	4.1373583E-5	9.945613	17
CTTACAC	220	1.8189894E-12	9.935618	3
CTAGCCA	235	0.0	9.705841	4
CTAGACA	245	0.0	9.697587	4
TCCTAGG	335	0.0	9.645481	2
ATAGCAC	200	2.6375346E-10	9.503635	3
TAGGTCG	90	0.0011160152	9.49807	5
CTTATAC	315	0.0	9.362742	1
TAATACT	305	0.0	9.347838	4
>>END_MODULE