FastQCFastQC Report
Mon 15 Aug 2016
SRR2921168_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921168_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873812
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.013.3361431
TAACGCT550.003079851412.0854934
GTATCAA26900.09.9946441
ACAACGT1151.0317401E-59.9092938
GTCCTAG2600.09.8656021
GTCCTAC1701.6658305E-89.500211
CTTGGAC5100.09.4964073
ACTAGAC800.00453153869.4964073
GGTCTAG1752.671004E-89.2287751
TGGACTG5100.09.1235225
TCCTAGG2304.1836756E-119.0858912
TAGACTG2106.730261E-109.0437255
GTCTTGG6900.08.8117881
CCTAGAC2054.1727617E-98.8015473
TTGGACT4100.08.8009354
TAATACC1304.393905E-58.7653044
ATTAAGC1752.6422276E-78.6824293
TAGAGGT5600.08.647914
TCTAATA2002.54513E-88.5489982
TTACATG4000.08.5467663
Produced by FastQC (version 0.11.5)