##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921168_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2873812
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.410675785333208	30.0	26.0	31.0	2.0	34.0
2	25.9907902813406	30.0	26.0	31.0	2.0	34.0
3	25.852665379642094	30.0	25.0	31.0	2.0	34.0
4	29.681191393173943	35.0	30.0	37.0	2.0	37.0
5	29.3360786996505	35.0	28.0	37.0	2.0	37.0
6	29.68524176250917	35.0	30.0	37.0	2.0	37.0
7	29.504281421331665	35.0	30.0	37.0	2.0	37.0
8	29.17933810562417	35.0	29.0	37.0	2.0	37.0
9	30.679207616921357	37.0	30.0	39.0	2.0	39.0
10	30.004930385146974	35.0	28.0	39.0	2.0	39.0
11	30.518259719146556	37.0	30.0	39.0	2.0	39.0
12	30.22494025357261	37.0	29.0	39.0	2.0	39.0
13	30.226362754418172	37.0	29.0	39.0	2.0	39.0
14	31.25023592357468	37.0	30.0	40.0	2.0	41.0
15	30.91107977835711	37.0	29.0	40.0	2.0	41.0
16	30.823048619742696	37.0	28.0	40.0	2.0	41.0
17	31.10882305453523	37.0	30.0	40.0	2.0	41.0
18	31.156515805487626	38.0	30.0	40.0	2.0	41.0
19	30.866937712000645	37.0	29.0	40.0	2.0	41.0
20	30.72344502702334	37.0	29.0	40.0	2.0	41.0
21	30.671382470391244	37.0	29.0	40.0	2.0	41.0
22	29.937861975661594	37.0	27.0	40.0	2.0	41.0
23	29.99541619284769	37.0	27.0	40.0	2.0	41.0
24	29.609143534789332	37.0	27.0	40.0	2.0	41.0
25	29.131412910795834	37.0	25.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	289242.0
3	6.0
4	2444.0
5	13490.0
6	15601.0
7	13300.0
8	11262.0
9	10337.0
10	9541.0
11	9205.0
12	9041.0
13	8707.0
14	8637.0
15	8984.0
16	9429.0
17	10245.0
18	11940.0
19	14746.0
20	18424.0
21	23257.0
22	28074.0
23	32976.0
24	38349.0
25	42542.0
26	47413.0
27	52973.0
28	58552.0
29	67887.0
30	80142.0
31	94646.0
32	116446.0
33	144597.0
34	181143.0
35	228781.0
36	291178.0
37	371735.0
38	498540.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.16191576829493	17.435071829127782	11.666254755829698	25.736757646747588
2	16.131144824167002	19.347304901375733	38.361278094181	26.160272180276262
3	19.451011482388022	22.795844721000556	29.79281725173725	27.960326544874164
4	13.764418957679798	15.073043987869736	34.50986506229045	36.652671992160016
5	14.138561529231291	36.95804692263361	33.875669219654384	15.027722328480708
6	34.972550195891074	33.67767523803923	16.77989109159295	14.569883474476745
7	30.42942821201202	31.011220706370345	19.94623665268356	18.61311442893408
8	27.110601672933733	34.59269652169253	19.97105528169774	18.325646523676
9	27.341399137601343	15.641111033392457	18.652859657859285	38.36463017114691
10	16.130130086240467	27.998659572002595	32.6624233699376	23.208786971819347
11	36.35980507171302	22.38181263261419	21.668701954816257	19.589680340856532
12	23.767039457451773	24.183423227915565	29.091670897784656	22.957866416848006
13	29.019983818066347	20.88642196566994	25.048728500213148	25.044865716050563
14	22.888234446573584	20.405282467805915	25.524561192251284	31.181921893369214
15	24.814458443458847	27.694176150945758	22.784332626326073	24.707032779269316
16	24.77654780543804	26.87723165025685	24.391149768489672	23.955070775815436
17	23.739146145456534	26.87025016039493	25.396705489840727	23.993898204307808
18	23.73617400729033	26.87714857814899	25.948646089043937	23.438031325516747
19	24.925965047219144	26.15141796641846	26.04429394025347	22.87832304610892
20	25.165922697250505	25.639554996544074	25.766794300403085	23.427728005802333
21	25.495457178020704	25.458946143265266	25.331592591148805	23.714004087565225
22	24.772926814245245	24.720171295360263	26.517617246182795	23.9892846442117
23	24.787607136107884	25.10299022284589	25.826645102844054	24.282757538202176
24	24.433035040011024	25.421321627112697	25.84982971736493	24.295813615511346
25	24.331749448565464	25.164678682095065	26.33401007668165	24.169561792657817
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	800.0
1	800.0
2	957.0
3	1114.0
4	1114.0
5	1114.0
6	1421.5
7	1729.0
8	1729.0
9	1729.0
10	2264.0
11	2799.0
12	2799.0
13	2799.0
14	4451.5
15	6104.0
16	6104.0
17	6104.0
18	11376.5
19	16649.0
20	16649.0
21	16649.0
22	27332.0
23	38015.0
24	38015.0
25	38015.0
26	56737.0
27	75459.0
28	75459.0
29	75459.0
30	101730.0
31	128001.0
32	128001.0
33	128001.0
34	160713.0
35	193425.0
36	193425.0
37	193425.0
38	230372.0
39	267319.0
40	267319.0
41	267319.0
42	303301.5
43	339284.0
44	339284.0
45	339284.0
46	365110.5
47	390937.0
48	390937.0
49	390937.0
50	397578.0
51	404219.0
52	404219.0
53	404219.0
54	381236.0
55	358253.0
56	358253.0
57	358253.0
58	317338.5
59	276424.0
60	276424.0
61	276424.0
62	231136.0
63	185848.0
64	185848.0
65	185848.0
66	145359.5
67	104871.0
68	104871.0
69	104871.0
70	77420.0
71	49969.0
72	49969.0
73	49969.0
74	35322.0
75	20675.0
76	20675.0
77	20675.0
78	14356.0
79	8037.0
80	8037.0
81	8037.0
82	5370.0
83	2703.0
84	2703.0
85	2703.0
86	1778.5
87	854.0
88	854.0
89	854.0
90	562.5
91	271.0
92	271.0
93	271.0
94	157.0
95	43.0
96	43.0
97	43.0
98	26.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035562521139169856
2	0.055605585890795915
3	0.01583262927428795
4	0.011761381746613906
5	0.010995848023461522
6	7.30736735736367E-4
7	0.0037232776535138694
8	0.0020182252701290136
9	0.0069246004957874766
10	0.0035840897038497996
11	0.012004960658526027
12	0.00908201371558056
13	0.00824688601759614
14	0.015937020236536002
15	0.006855006520955441
16	0.03263957419622439
17	0.04227833971046122
18	0.024044718304468072
19	0.01764207261992086
20	0.016911335884184493
21	0.02463626709054037
22	0.006228660747467127
23	0.016737350947104403
24	0.012318133545270185
25	0.47132519454995664
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2873812.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.73732056869276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.45449365052416	37.486013646394824
2	12.397053114188509	12.827806221517635
3	5.540023259137618	8.59877877948051
4	2.9569495620100814	6.119385895806849
5	1.8441125433705086	4.770472091055366
6	1.1620662496165557	3.6073316447082164
7	0.8235509201829175	2.982582257350186
8	0.5613022723410498	2.3232220480035664
9	0.41213915915352883	1.9190678216433845
>10	1.7693939031909818	14.952981496344794
>50	0.056526529078220096	1.9628689786001277
>100	0.021139769729044734	1.8664783052069183
>500	9.714969264942817E-4	0.37908807094799335
>1k	2.7757055037220185E-4	0.2039227429394983
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.435789119121223E-4
2	0.0	3.479698741601747E-5	0.0	0.0	2.435789119121223E-4
3	0.0	3.479698741601747E-5	0.0	0.0	2.435789119121223E-4
4	0.0	3.479698741601747E-5	0.0	0.0	2.7837589932813977E-4
5	0.0	3.479698741601747E-5	0.0	0.0	2.7837589932813977E-4
6	0.0	3.479698741601747E-5	6.959397483203494E-5	0.0	4.175638489922096E-4
7	0.0	3.479698741601747E-5	6.959397483203494E-5	0.0	4.175638489922096E-4
8	0.0	3.479698741601747E-5	6.959397483203494E-5	0.0	4.175638489922096E-4
9	0.0	3.479698741601747E-5	6.959397483203494E-5	3.479698741601747E-5	4.5236083640822714E-4
10	0.0	3.479698741601747E-5	6.959397483203494E-5	3.479698741601747E-5	5.91548786072297E-4
11	0.0	3.479698741601747E-5	6.959397483203494E-5	3.479698741601747E-5	5.91548786072297E-4
12	0.0	3.479698741601747E-5	6.959397483203494E-5	3.479698741601747E-5	8.351276979844192E-4
13	0.0	3.479698741601747E-5	6.959397483203494E-5	3.479698741601747E-5	9.743156476484892E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1325	0.0	13.336143	1
TAACGCT	55	0.0030798514	12.085493	4
GTATCAA	2690	0.0	9.994644	1
ACAACGT	115	1.0317401E-5	9.909293	8
GTCCTAG	260	0.0	9.865602	1
GTCCTAC	170	1.6658305E-8	9.50021	1
CTTGGAC	510	0.0	9.496407	3
ACTAGAC	80	0.0045315386	9.496407	3
GGTCTAG	175	2.671004E-8	9.228775	1
TGGACTG	510	0.0	9.123522	5
TCCTAGG	230	4.1836756E-11	9.085891	2
TAGACTG	210	6.730261E-10	9.043725	5
GTCTTGG	690	0.0	8.811788	1
CCTAGAC	205	4.1727617E-9	8.801547	3
TTGGACT	410	0.0	8.800935	4
TAATACC	130	4.393905E-5	8.765304	4
ATTAAGC	175	2.6422276E-7	8.682429	3
TAGAGGT	560	0.0	8.64791	4
TCTAATA	200	2.54513E-8	8.548998	2
TTACATG	400	0.0	8.546766	3
>>END_MODULE