FastQCFastQC Report
Mon 15 Aug 2016
SRR2921168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873812
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36890.12836608657768844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.013.60251
GCGCATA700.001471548110.875181
GTCGACT750.00265285110.1314398
CTCCTAA3150.09.9689161
CGTAGGA1151.0211204E-59.9179242
TAGGACG1151.0213318E-59.917754
GTATCAA32100.09.5750711
CGTGCGC1606.6405846E-89.50004410
TACACCG1502.6562157E-79.4985555
TCGCAAT800.00454130779.49392717
CGTTTTA800.00454130779.49392717
GGTCCGC1002.7753945E-49.4935976
CCTAGAC2357.2759576E-129.302453
CTATACT2252.7284841E-119.2933014
GTAGGAC2054.2018655E-109.2728573
TATATAA3400.09.2251272
GTATTAG1252.7021459E-59.1351531
CCCCTAG2201.6189006E-109.0832471
CTATTAC1054.4204373E-49.0626511
AGTACTG4000.09.0236275
Produced by FastQC (version 0.11.5)