##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921168_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2873812
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.797175319749517	31.0	30.0	33.0	26.0	34.0
2	30.038080431148593	31.0	30.0	34.0	26.0	34.0
3	30.17932314291958	31.0	30.0	34.0	26.0	34.0
4	33.883717863242275	35.0	35.0	37.0	30.0	37.0
5	33.49518792461024	35.0	35.0	37.0	28.0	37.0
6	33.475434718763786	37.0	35.0	37.0	28.0	37.0
7	33.33590575862304	37.0	35.0	37.0	28.0	37.0
8	33.28301712150969	37.0	35.0	37.0	27.0	37.0
9	34.69565650084278	39.0	35.0	39.0	27.0	39.0
10	34.56255732803677	38.0	35.0	39.0	27.0	39.0
11	34.6184586883206	39.0	35.0	39.0	27.0	39.0
12	34.481108715531846	38.0	35.0	39.0	27.0	39.0
13	34.47407485249557	38.0	35.0	39.0	27.0	39.0
14	35.451465509922016	39.0	35.0	41.0	27.0	41.0
15	35.45514703119063	39.0	35.0	41.0	27.0	41.0
16	35.43046587598632	39.0	35.0	41.0	27.0	41.0
17	35.40479996603814	39.0	35.0	41.0	27.0	41.0
18	35.35474693542932	39.0	35.0	41.0	26.0	41.0
19	35.38764226748305	39.0	35.0	41.0	26.0	41.0
20	35.32964230088816	39.0	35.0	41.0	26.0	41.0
21	35.25706065671658	39.0	35.0	41.0	26.0	41.0
22	35.17913628309716	39.0	35.0	41.0	25.0	41.0
23	35.06345926595059	39.0	35.0	41.0	25.0	41.0
24	34.99344877117919	39.0	35.0	41.0	25.0	41.0
25	34.85963173652278	39.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77846.0
3	12.0
4	1185.0
5	10491.0
6	11912.0
7	9300.0
8	7164.0
9	6037.0
10	5644.0
11	5130.0
12	4860.0
13	4634.0
14	4615.0
15	4668.0
16	4532.0
17	4989.0
18	5688.0
19	6521.0
20	8189.0
21	10877.0
22	13623.0
23	16691.0
24	20057.0
25	23589.0
26	27151.0
27	30865.0
28	35605.0
29	43495.0
30	55045.0
31	71328.0
32	91757.0
33	118770.0
34	156380.0
35	207525.0
36	283281.0
37	429627.0
38	1054729.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94462105014557	18.532277739809295	9.103240139863356	31.41986107018178
2	15.243516277334773	20.46939744144711	35.13169963797214	29.155386643245972
3	18.489500839974575	23.359682473598063	27.827873788685654	30.32294289774171
4	13.6148678642242	15.675527942525594	33.05223980628326	37.65736438696695
5	14.76157016822435	37.261028733306915	32.75374843575796	15.223652662710768
6	35.016092645196586	34.478453185174516	16.038776854569047	14.466677315059856
7	30.35201580645307	31.351019551716686	19.391195446527774	18.905769195302465
8	28.131551887030458	34.94417466015008	18.360299196678838	18.563974256140614
9	27.401071260427358	15.437758432965843	18.438895250940302	38.7222750556665
10	16.600430578072114	28.44386774217233	31.306999660033313	23.648702019722244
11	36.771299668197216	22.120649547011777	21.368482041413582	19.73956874337743
12	23.851455836359513	24.223052864975163	28.73952784663715	23.185963452028176
13	29.125863248493772	20.687863681379444	24.98519350059052	25.20107956953626
14	23.00081711207456	20.12748619491413	25.341754427010166	31.529942266001136
15	24.906687586838668	27.723184079879044	22.399677844201335	24.97045048908095
16	25.092223315265066	26.79836769690691	23.848891910899106	24.260517076928913
17	23.76842851018579	26.793265242493586	25.354999359734542	24.083306887586087
18	23.772163554005246	26.73121311149103	25.990110982627577	23.50651235187614
19	25.024663214454613	25.995984985278625	25.73385024878963	23.24550155147713
20	25.177822109340287	25.523949308911302	25.634882606612365	23.66334597513605
21	25.337049540653528	25.225653801810243	25.46240019710886	23.97489646042737
22	25.29040968618042	24.837313856116587	25.292497525041018	24.579778932661977
23	24.775074006784926	25.0119096377765	25.603615936539466	24.609400418899106
24	24.42775346124856	25.45229556310145	25.569741035343608	24.55020994030638
25	24.545854738345618	25.217605280594313	25.67625548478933	24.560284496270743
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	483.0
1	483.0
2	522.0
3	561.0
4	561.0
5	561.0
6	835.5
7	1110.0
8	1110.0
9	1110.0
10	1538.0
11	1966.0
12	1966.0
13	1966.0
14	3118.5
15	4271.0
16	4271.0
17	4271.0
18	8834.0
19	13397.0
20	13397.0
21	13397.0
22	23162.5
23	32928.0
24	32928.0
25	32928.0
26	51143.5
27	69359.0
28	69359.0
29	69359.0
30	94300.5
31	119242.0
32	119242.0
33	119242.0
34	150090.0
35	180938.0
36	180938.0
37	180938.0
38	217305.5
39	253673.0
40	253673.0
41	253673.0
42	291256.5
43	328840.0
44	328840.0
45	328840.0
46	359022.5
47	389205.0
48	389205.0
49	389205.0
50	401081.5
51	412958.0
52	412958.0
53	412958.0
54	393564.5
55	374171.0
56	374171.0
57	374171.0
58	332543.0
59	290915.0
60	290915.0
61	290915.0
62	245171.5
63	199428.0
64	199428.0
65	199428.0
66	156335.0
67	113242.0
68	113242.0
69	113242.0
70	83033.5
71	52825.0
72	52825.0
73	52825.0
74	37279.0
75	21733.0
76	21733.0
77	21733.0
78	14982.0
79	8231.0
80	8231.0
81	8231.0
82	5586.5
83	2942.0
84	2942.0
85	2942.0
86	1951.5
87	961.0
88	961.0
89	961.0
90	639.0
91	317.0
92	317.0
93	317.0
94	208.0
95	99.0
96	99.0
97	99.0
98	58.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10473893212221258
2	0.0
3	0.03848546808211532
4	0.05487484915505955
5	0.0631217351726557
6	0.004836781250826428
7	0.0017050523833848558
8	0.004071247527674044
9	3.479698741601747E-5
10	3.479698741601747E-5
11	0.004071247527674044
12	0.0
13	0.002574977068785293
14	0.05223027811144223
15	0.02324438759389967
16	0.002818555980697415
17	1.3918794966406988E-4
18	0.004140841502506079
19	0.0034449017541857297
20	4.523608364082271E-4
21	0.009499577564572769
22	9.395186602324716E-4
23	0.003096931880025555
24	0.004801984263410411
25	0.16497251733933882
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2873812.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.06241013751427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.81912016640085	22.17040765672399
2	15.578675384345642	11.54766512987632
3	7.849624566207619	8.72778015294883
4	4.5422317367894935	6.733842222741039
5	2.9837501630798613	5.529248614597045
6	2.0474257408642456	4.552951952040881
7	1.4257205686116965	3.6988448318763725
8	1.0395080944801807	3.0821340271112314
9	0.7862712438809131	2.6226996588043114
>10	3.727701569257405	23.0364452011958
>50	0.14398411405196188	3.5949415324176455
>100	0.05248775946801485	3.349908014829576
>500	0.0023641165959992255	0.5828473013572808
>1k	0.0011347759660386585	0.7702837034796055
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3689	0.12836608657768844	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.479698741601747E-4
2	0.0	0.0	0.0	0.0	3.479698741601747E-4
3	0.0	0.0	0.0	0.0	3.8276686157619216E-4
4	0.0	0.0	0.0	0.0	3.8276686157619216E-4
5	0.0	0.0	0.0	0.0	4.175638489922096E-4
6	0.0	0.0	6.959397483203494E-5	0.0	4.871578238242446E-4
7	0.0	0.0	6.959397483203494E-5	0.0	4.871578238242446E-4
8	0.0	0.0	6.959397483203494E-5	0.0	5.219548112402621E-4
9	0.0	0.0	6.959397483203494E-5	0.0	5.219548112402621E-4
10	0.0	0.0	6.959397483203494E-5	0.0	5.567517986562795E-4
11	0.0	0.0	6.959397483203494E-5	0.0	5.567517986562795E-4
12	0.0	0.0	6.959397483203494E-5	0.0	6.959397483203494E-4
13	0.0	0.0	6.959397483203494E-5	0.0	8.699246854004368E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1595	0.0	13.6025	1
GCGCATA	70	0.0014715481	10.87518	1
GTCGACT	75	0.002652851	10.131439	8
CTCCTAA	315	0.0	9.968916	1
CGTAGGA	115	1.0211204E-5	9.917924	2
TAGGACG	115	1.0213318E-5	9.91775	4
GTATCAA	3210	0.0	9.575071	1
CGTGCGC	160	6.6405846E-8	9.500044	10
TACACCG	150	2.6562157E-7	9.498555	5
TCGCAAT	80	0.0045413077	9.493927	17
CGTTTTA	80	0.0045413077	9.493927	17
GGTCCGC	100	2.7753945E-4	9.493597	6
CCTAGAC	235	7.2759576E-12	9.30245	3
CTATACT	225	2.7284841E-11	9.293301	4
GTAGGAC	205	4.2018655E-10	9.272857	3
TATATAA	340	0.0	9.225127	2
GTATTAG	125	2.7021459E-5	9.135153	1
CCCCTAG	220	1.6189006E-10	9.083247	1
CTATTAC	105	4.4204373E-4	9.062651	1
AGTACTG	400	0.0	9.023627	5
>>END_MODULE