FastQCFastQC Report
Mon 15 Aug 2016
SRR2921167_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921167_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472393
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50860.34542408174991324No Hit
TATCAACGCAGAGTACTTTTTTTTT28640.19451328551548397No Hit
GGTATCAACGCAGAGTACTTTTTTT25180.17101412462569435No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA14950.10153539170588287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC1500.013.2957683
TCGTTCG1052.7233364E-711.76055914
CGTTCGC907.4692052E-611.60978215
GGTATCA16700.011.2637151
ATTACAC1053.478437E-610.8536893
CCCTATA909.524412E-510.5543472
TAGGACC909.5474024E-510.5518384
TATACTG2250.010.5518385
TAGGACA1659.331416E-1010.3599864
GTGTAAG1303.8381404E-710.23098851
AACCGAG750.002654428610.1301097
GAACCGC750.002654428610.1301096
GTATAGG1701.5570549E-910.0590391
TTCGCGC856.6015014E-410.05733117
TCGCGCA856.6097535E-410.05596418
GACCGTG1054.1181815E-59.9492157
CATCTAG1252.5679492E-69.8802121
GTCTTAG1356.436894E-79.8520631
GTATCAA31950.09.7529491
GTGCTAG1002.7540198E-49.5002041
Produced by FastQC (version 0.11.5)