##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921167_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1472393
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.57058407639808	31.0	27.0	31.0	2.0	34.0
2	26.157523840442057	31.0	26.0	31.0	2.0	34.0
3	26.016307466824415	31.0	26.0	31.0	2.0	34.0
4	29.829059225356275	35.0	30.0	37.0	2.0	37.0
5	29.52676493300362	35.0	30.0	37.0	2.0	37.0
6	29.859600663681505	35.0	30.0	37.0	2.0	37.0
7	29.726433771418364	35.0	30.0	37.0	2.0	37.0
8	29.35757980376163	35.0	29.0	37.0	2.0	37.0
9	30.84688123347503	37.0	30.0	39.0	2.0	39.0
10	30.20211383781368	37.0	28.0	39.0	2.0	39.0
11	30.682014244838165	37.0	30.0	39.0	2.0	39.0
12	30.42978335267826	37.0	30.0	39.0	2.0	39.0
13	30.417957026418897	37.0	30.0	39.0	2.0	39.0
14	31.45926053709845	38.0	30.0	40.0	2.0	41.0
15	31.12057378702561	38.0	30.0	40.0	2.0	41.0
16	31.043975351689394	38.0	29.0	40.0	2.0	41.0
17	31.348921110056892	38.0	30.0	40.0	2.0	41.0
18	31.40048478904749	38.0	30.0	40.0	2.0	41.0
19	31.113892826168012	38.0	30.0	40.0	2.0	41.0
20	30.98655861580434	38.0	30.0	40.0	2.0	41.0
21	30.9406238687633	38.0	30.0	40.0	2.0	41.0
22	30.20630565344986	37.0	27.0	40.0	2.0	41.0
23	30.284752100831774	37.0	27.0	40.0	2.0	41.0
24	29.914240287749262	37.0	27.0	40.0	2.0	41.0
25	29.43763655491435	37.0	26.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	147684.0
3	7.0
4	1125.0
5	6891.0
6	8446.0
7	7186.0
8	6073.0
9	5296.0
10	5166.0
11	4668.0
12	4502.0
13	4371.0
14	4368.0
15	4495.0
16	4578.0
17	5049.0
18	5683.0
19	7398.0
20	9112.0
21	11080.0
22	13318.0
23	15455.0
24	17651.0
25	19887.0
26	22073.0
27	25051.0
28	27516.0
29	32139.0
30	37772.0
31	44960.0
32	55945.0
33	70612.0
34	90143.0
35	115784.0
36	152100.0
37	198035.0
38	280774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.10151465228217	17.892530681984166	12.275986697962914	24.729967967770754
2	16.217402619817904	19.863158445504947	38.07766767303444	25.84177126164271
3	19.013475576663527	23.779070912683505	29.547302405416588	27.66015110523638
4	13.486164440040021	15.69793860718611	33.934971951228626	36.88092500154524
5	13.703050646684932	38.06808225077057	32.861932325280954	15.366934777263532
6	34.2338450880714	34.35473738186683	16.36066653762433	15.05075099243744
7	29.998553334089493	32.59711848601319	19.048514926128348	18.35581325376897
8	26.71385133230687	34.71597851356758	19.424044619189047	19.146125534936505
9	27.30151312831366	16.688967163254578	18.376175190889988	37.63334451754177
10	16.502653929615683	28.803914843328158	31.15091911202877	23.54251211502739
11	35.21205016009553	23.825637834981382	20.99520053034615	19.96711147457694
12	23.92381058468577	25.900475317840787	27.857986059697964	22.317728037775474
13	28.527832794038137	22.279955334947143	24.530935439437286	24.66127643157743
14	22.956395218512977	21.76815575290488	25.01151355338824	30.263935475193897
15	24.499356111237898	28.494047424988317	22.685939860246254	24.32065660352753
16	23.990670834400518	28.040827667919206	24.093799827846595	23.87470166983368
17	23.421110919361393	27.85145798484332	25.00193637019593	23.725494725599358
18	23.410320004510645	27.016119445163383	26.119832372443486	23.453728177882482
19	24.37851059913093	26.410777713397216	26.675424838110818	22.535286849361036
20	25.1335612506971	25.688523955437244	26.214074316095072	22.963840477770585
21	25.303874609308924	25.41073086828013	25.839990054827517	23.445404467583426
22	24.52063456701743	24.8913442879935	27.035048980819948	23.55297216416912
23	24.68838517290802	25.203746849888258	26.091144365120876	24.016723612082842
24	24.3198560002717	25.55309140926149	26.228463719879773	23.898588870587037
25	23.870224194684177	25.265640950057044	26.81943238949937	24.044702465759407
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2226.0
1	2226.0
2	2300.0
3	2374.0
4	2374.0
5	2374.0
6	2995.5
7	3617.0
8	3617.0
9	3617.0
10	4327.5
11	5038.0
12	5038.0
13	5038.0
14	6253.0
15	7468.0
16	7468.0
17	7468.0
18	11342.5
19	15217.0
20	15217.0
21	15217.0
22	21074.5
23	26932.0
24	26932.0
25	26932.0
26	36896.0
27	46860.0
28	46860.0
29	46860.0
30	58725.0
31	70590.0
32	70590.0
33	70590.0
34	82931.5
35	95273.0
36	95273.0
37	95273.0
38	110456.0
39	125639.0
40	125639.0
41	125639.0
42	142165.0
43	158691.0
44	158691.0
45	158691.0
46	174704.5
47	190718.0
48	190718.0
49	190718.0
50	193884.0
51	197050.0
52	197050.0
53	197050.0
54	186616.5
55	176183.0
56	176183.0
57	176183.0
58	158840.5
59	141498.0
60	141498.0
61	141498.0
62	120673.5
63	99849.0
64	99849.0
65	99849.0
66	79464.0
67	59079.0
68	59079.0
69	59079.0
70	43866.0
71	28653.0
72	28653.0
73	28653.0
74	20234.0
75	11815.0
76	11815.0
77	11815.0
78	8373.0
79	4931.0
80	4931.0
81	4931.0
82	3411.5
83	1892.0
84	1892.0
85	1892.0
86	1244.0
87	596.0
88	596.0
89	596.0
90	385.5
91	175.0
92	175.0
93	175.0
94	96.5
95	18.0
96	18.0
97	18.0
98	14.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029747492687074713
2	0.05188832057745452
3	0.011817497094865298
4	0.00923666439598667
5	0.007810414746606375
6	5.433331997639217E-4
7	0.0028524992987605893
8	0.0014941662993507847
9	0.005365415347668727
10	0.0032599991985835302
11	0.009983747545662062
12	0.007810414746606375
13	0.006859581647019511
14	0.014602079743655395
15	0.006044581847373629
16	0.030562492486720598
17	0.03850874053326795
18	0.02173332799055687
19	0.016571662592799612
20	0.015077496293448829
21	0.02186916129049785
22	0.0055691652975801975
23	0.015145412943419319
24	0.011409997195042357
25	0.4673344684469432
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1472393.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.62543073494588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.69896071753115	40.804629046479405
2	11.79355724955247	12.884562893080945
3	5.155664810571155	8.448912330073576
4	2.7275316608573696	5.959703672701446
5	1.633617951461282	4.461854212745623
6	1.0330450490055874	3.385831846232009
7	0.6890255672464162	2.6346822878758136
8	0.4581255163007879	2.0020242926880005
9	0.33880104113598053	1.6656437524950947
>10	1.4015289285891162	12.428335657648912
>50	0.047904457322765485	1.7821191892734698
>100	0.019794888811160052	1.8341981657121642
>500	0.0014138830401422993	0.528842056924555
>1k	8.997437528178268E-4	0.8215588035148988
>5k	1.2853482183111813E-4	0.357101792554207
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5086	0.34542408174991324	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2864	0.19451328551548397	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2518	0.17101412462569435	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1495	0.10153539170588287	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATAC	150	0.0	13.295768	3
TCGTTCG	105	2.7233364E-7	11.760559	14
CGTTCGC	90	7.4692052E-6	11.609782	15
GGTATCA	1670	0.0	11.263715	1
ATTACAC	105	3.478437E-6	10.853689	3
CCCTATA	90	9.524412E-5	10.554347	2
TAGGACC	90	9.5474024E-5	10.551838	4
TATACTG	225	0.0	10.551838	5
TAGGACA	165	9.331416E-10	10.359986	4
GTGTAAG	130	3.8381404E-7	10.2309885	1
AACCGAG	75	0.0026544286	10.130109	7
GAACCGC	75	0.0026544286	10.130109	6
GTATAGG	170	1.5570549E-9	10.059039	1
TTCGCGC	85	6.6015014E-4	10.057331	17
TCGCGCA	85	6.6097535E-4	10.055964	18
GACCGTG	105	4.1181815E-5	9.949215	7
CATCTAG	125	2.5679492E-6	9.880212	1
GTCTTAG	135	6.436894E-7	9.852063	1
GTATCAA	3195	0.0	9.752949	1
GTGCTAG	100	2.7540198E-4	9.500204	1
>>END_MODULE