FastQCFastQC Report
Mon 15 Aug 2016
SRR2921167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472393
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98580.6695223354090926No Hit
TATCAACGCAGAGTACTTTTTTTTT62630.4253619787651802No Hit
GGTATCAACGCAGAGTACTTTTTTT62020.4212190631169803No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46390.3150653392131041No Hit
GGTATCAACGCAGAGTACATGGGGG18670.12680038549490522No Hit
CCCATGTACTCTGCGTTGATACCAC17750.12055205369762015No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15450.10493122420440737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC400.00527986514.24961810
TACCGCT655.488351E-513.1445597
AGCGTAA604.0927212E-412.66632710
GGTATCA20600.012.1007051
TCGTTCG951.0399726E-611.99967914
ACCGTGC951.04211E-611.997648
AAGGTAT658.0270687E-411.6915975
GTTCGCG1001.9432355E-611.39311216
CGTACAC600.00585431711.0886893
CGGAAAT600.00587657911.08303610
TTCGCGC1053.4903824E-610.85058417
TCGCGCA1053.4903824E-610.85058418
CTCCTAG1502.0791049E-910.7828671
TATACAC2300.010.744323
CAGCGTA803.771351E-410.6872139
CGCCCAA803.771351E-410.68721310
GTCTTAG1252.193101E-710.6560111
TGTACCG1451.4437319E-810.4821215
TCTTATA1106.008606E-610.3686442
CGTTCGC1106.0723014E-610.35948715
Produced by FastQC (version 0.11.5)