##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921167_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1472393
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.235494871274177	31.0	30.0	33.0	25.0	34.0
2	29.509287941466713	31.0	30.0	34.0	25.0	34.0
3	29.628163133076562	31.0	30.0	34.0	25.0	34.0
4	33.277893877517755	35.0	35.0	37.0	28.0	37.0
5	32.84052016003879	35.0	33.0	37.0	26.0	37.0
6	32.77047771892423	35.0	33.0	37.0	26.0	37.0
7	32.61966472266575	35.0	33.0	37.0	25.0	37.0
8	32.53229946080972	36.0	33.0	37.0	25.0	37.0
9	33.86683107023736	38.0	34.0	39.0	23.0	39.0
10	33.72847670424947	38.0	34.0	39.0	23.0	39.0
11	33.74907786168502	38.0	34.0	39.0	23.0	39.0
12	33.62686185006313	38.0	34.0	39.0	22.0	39.0
13	33.57978610330258	38.0	34.0	39.0	21.0	39.0
14	34.484432485077015	39.0	34.0	41.0	18.0	41.0
15	34.45847066645929	39.0	34.0	41.0	18.0	41.0
16	34.43569345955869	39.0	34.0	41.0	18.0	41.0
17	34.428798561253686	39.0	34.0	41.0	18.0	41.0
18	34.38446325131945	39.0	34.0	41.0	17.0	41.0
19	34.41378015244571	39.0	34.0	41.0	18.0	41.0
20	34.35843894938376	39.0	34.0	41.0	18.0	41.0
21	34.282639893017695	39.0	34.0	41.0	18.0	41.0
22	34.19774475972108	39.0	34.0	41.0	16.0	41.0
23	34.06531340477712	39.0	34.0	41.0	10.0	41.0
24	33.971722902784784	39.0	34.0	41.0	2.0	41.0
25	33.82558800537628	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56410.0
3	0.0
4	841.0
5	7471.0
6	8779.0
7	6680.0
8	5331.0
9	4547.0
10	3913.0
11	3518.0
12	3349.0
13	3135.0
14	3034.0
15	2926.0
16	3038.0
17	3117.0
18	3436.0
19	4225.0
20	5504.0
21	6940.0
22	8301.0
23	9885.0
24	11421.0
25	13087.0
26	14840.0
27	16738.0
28	19152.0
29	23112.0
30	29083.0
31	37204.0
32	47124.0
33	60888.0
34	79114.0
35	104414.0
36	142734.0
37	215172.0
38	503930.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.76467848899857	19.060128012944062	9.577108593455227	30.59808490460214
2	15.128433780926695	21.047098159255036	34.45853111227777	29.3659369475405
3	18.2809637951745	24.021977812926334	27.515680967056383	30.181377424842783
4	13.264128710812475	16.390432583254167	32.21915521977088	38.12628348616247
5	14.451473208763815	38.01139584100736	31.796149825949733	15.740981124279093
6	34.41047400211779	34.93584497107573	15.508733655597084	15.1449473712094
7	29.977247721376276	32.82318423233133	18.440484114155314	18.759083932137084
8	28.30326572224415	34.334198423819174	17.754821900260342	19.60771395367634
9	27.415932713570847	15.867173959108715	18.39136588625855	38.325527441061894
10	17.41404462941934	28.87899418089748	29.658677852093735	24.048283337589446
11	35.774114847095134	23.072388011041316	20.659409028668303	20.494088113195243
12	24.398649001998788	25.57557662933741	27.46820991406506	22.55756445459874
13	29.019237352140532	21.350513195285885	24.880802312503057	24.749447140070526
14	23.26332849721215	21.07694869067965	25.203303965506112	30.456418846602084
15	24.875820279064712	27.957012621768136	22.63712076953385	24.530046329633304
16	24.561579861063944	27.283995729224113	23.85582871254581	24.298595697166128
17	23.45662494311969	27.262002594421315	25.364747111838575	23.916625350620418
18	23.50844375797662	26.089762007130478	26.850627364119784	23.551166870773113
19	24.51728719068888	25.60632858431411	27.09956639389691	22.7768178311001
20	25.017386721434836	24.906478455407136	26.954851574449123	23.1212832487089
21	25.01720285924087	24.716483973320454	26.758274863990422	23.50803830344825
22	25.093623547086157	24.489909575342335	26.535093379045982	23.881373498525523
23	24.497919956531337	24.481755047289152	26.711833324843358	24.308491671336153
24	24.242438650317165	25.113142610291543	26.71062185474411	23.933796884647176
25	23.967900206542414	24.85876743977207	26.897863551386607	24.275468802298906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1424.0
1	1424.0
2	1452.5
3	1481.0
4	1481.0
5	1481.0
6	2102.0
7	2723.0
8	2723.0
9	2723.0
10	3385.0
11	4047.0
12	4047.0
13	4047.0
14	4719.0
15	5391.0
16	5391.0
17	5391.0
18	8753.5
19	12116.0
20	12116.0
21	12116.0
22	18688.0
23	25260.0
24	25260.0
25	25260.0
26	35615.0
27	45970.0
28	45970.0
29	45970.0
30	56480.0
31	66990.0
32	66990.0
33	66990.0
34	78307.5
35	89625.0
36	89625.0
37	89625.0
38	103701.0
39	117777.0
40	117777.0
41	117777.0
42	134275.5
43	150774.0
44	150774.0
45	150774.0
46	169150.0
47	187526.0
48	187526.0
49	187526.0
50	194268.0
51	201010.0
52	201010.0
53	201010.0
54	192599.0
55	184188.0
56	184188.0
57	184188.0
58	166775.0
59	149362.0
60	149362.0
61	149362.0
62	129390.5
63	109419.0
64	109419.0
65	109419.0
66	87344.5
67	65270.0
68	65270.0
69	65270.0
70	48150.5
71	31031.0
72	31031.0
73	31031.0
74	21888.0
75	12745.0
76	12745.0
77	12745.0
78	9055.5
79	5366.0
80	5366.0
81	5366.0
82	3676.0
83	1986.0
84	1986.0
85	1986.0
86	1332.0
87	678.0
88	678.0
89	678.0
90	443.0
91	208.0
92	208.0
93	208.0
94	113.0
95	18.0
96	18.0
97	18.0
98	13.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09834330915726984
2	0.0
3	0.03701457423391717
4	0.05535206972594952
5	0.06649040032110992
6	0.0067916649970490225
7	8.829164496163729E-4
8	0.0067916649970490225
9	6.791664997049021E-5
10	6.791664997049021E-5
11	0.007131248246901473
12	0.0
13	0.0033279158485540208
14	0.056234986175565896
15	0.022616244440173242
16	0.004142915648199903
17	2.0374994991147064E-4
18	0.008285831296399806
19	0.006044581847373629
20	3.395832498524511E-4
21	0.017250829092504517
22	4.7541654979343153E-4
23	0.004618332197993335
24	0.00923666439598667
25	0.16822954197690426
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1472393.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.48238401128291
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.40203077833766	25.66665357063843
2	14.82633002298568	12.004103709370382
3	7.266213986607344	8.8246099474198
4	4.286319058394557	6.940816564672361
5	2.6635554809732302	5.391353790805783
6	1.7709221379013862	4.301469002436361
7	1.280995981871087	3.630043987851102
8	0.8723041565835692	2.825036147316344
9	0.6331415074902988	2.306796987573435
>10	2.844664660193907	18.873630804676996
>50	0.1084907052790998	2.9633714318513804
>100	0.04046070279012026	2.9135424730476234
>500	0.0025393447733802615	0.7197281767448381
>1k	0.0015236068640281568	1.108992701969622
>5k	5.078689546760523E-4	1.529850703625502
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9858	0.6695223354090926	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6263	0.4253619787651802	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6202	0.4212190631169803	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4639	0.3150653392131041	No Hit
GGTATCAACGCAGAGTACATGGGGG	1867	0.12680038549490522	No Hit
CCCATGTACTCTGCGTTGATACCAC	1775	0.12055205369762015	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1545	0.10493122420440737	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACCGC	40	0.005279865	14.249618	10
TACCGCT	65	5.488351E-5	13.144559	7
AGCGTAA	60	4.0927212E-4	12.666327	10
GGTATCA	2060	0.0	12.100705	1
TCGTTCG	95	1.0399726E-6	11.999679	14
ACCGTGC	95	1.04211E-6	11.99764	8
AAGGTAT	65	8.0270687E-4	11.691597	5
GTTCGCG	100	1.9432355E-6	11.393112	16
CGTACAC	60	0.005854317	11.088689	3
CGGAAAT	60	0.005876579	11.083036	10
TTCGCGC	105	3.4903824E-6	10.850584	17
TCGCGCA	105	3.4903824E-6	10.850584	18
CTCCTAG	150	2.0791049E-9	10.782867	1
TATACAC	230	0.0	10.74432	3
CAGCGTA	80	3.771351E-4	10.687213	9
CGCCCAA	80	3.771351E-4	10.687213	10
GTCTTAG	125	2.193101E-7	10.656011	1
TGTACCG	145	1.4437319E-8	10.482121	5
TCTTATA	110	6.008606E-6	10.368644	2
CGTTCGC	110	6.0723014E-6	10.359487	15
>>END_MODULE