FastQCFastQC Report
Mon 15 Aug 2016
SRR2921166_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921166_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293618
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27720.12085709128547126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTG551.962625E-413.8166614
CGTGTAA604.103942E-412.66278510
TAAACCG550.003080787612.0848325
CTAGCCG752.0843436E-411.394274
GTACTAG855.307528E-511.1801161
GTATAGG1803.6379788E-1211.0869471
GTATTAC1053.4544282E-610.8606841
GGTATCA13050.010.1220741
GTATCAA25700.010.05775451
AGCGTGC1054.128278E-59.9475978
GCTAGCC2201.8189894E-129.9272593
AAGACCG1451.6297417E-79.8226475
ACCGTGG2151.0913936E-119.7162578
AGCCTCG2106.730261E-119.5006112
TGCTAGC2401.8189894E-129.5006112
CCTATAC1106.872925E-59.495643
ACCGTGT1502.6678026E-79.4954328
TATACCG800.0045355999.4952265
ACACCGG900.00111907239.4952256
TACACCG900.00111907239.4952255
Produced by FastQC (version 0.11.5)